Diffuse optical spectroscopy monitoring of oxygen state and hemoglobin concentration during SKBR-3 tumor model growth

Abstract

Tumor oxygenation and hemoglobin content are the key indicators of the tumor status which can be efficiently employed for prognosis of tumor development and choice of treatment strategy. We report on monitoring of these parameters in SKBR-3 (human breast adenocarcinoma) tumors established as subcutaneous tumor xenografts in athymic nude mice by diffuse optical spectroscopy (DOS). A simple continuous wave fiber probe DOS system is employed. Optical properties extraction approach is based on diffusion approximation. Statistically significant difference between measured values of normal tissue and tumor are demonstrated. Hemoglobin content in tumor increases from 7.0 ± 4.2 μM to 30.1 ± 16.1 μM with tumor growth from 150 ± 80 mm 3 to 1300 ± 650 mm 3 which is determined by gradual increase of deoxyhemoglobin content while measured oxyhemoglobin content does not demonstrate any statistically significant variations. Oxygenation in tumor falls quickly from 52.8 ± 24.7% to 20.2 ± 4.8% preceding acceleration of tumor growth. Statistical analysis indicated dependence of oxy-, deoxy-and total hemoglobin on tumor volume ( p < 0.01). DOS measurements of oxygen saturation are in agreement with independent measurements of oxygen partial pressure by polarography (Pearson's correlation coefficient equals 0.8).

The Multifaceted Role of Connexins in Tumor Microenvironment Initiation and Maintaining

“…The oxygenation levels in tumors are lower compared to normal tissues. For example, it was demonstrated with polarography method, that oxygenation levels in well-oxygenated tissues, such as muscle, lies in the range of 20 and 70 mmHg, while in breast tumor it ranges between 3 to 30 mmHg, which indicates hypoxic characteristics [159].…”

Probing depth in diffuse reflectance spectroscopy of biotissues: a Monte Carlo study

“…DRS is based concentration of oxy-, deoxyhemoglobin, methehemoglobin, bilirubin, melanin, water and lipid content, bound water index, etc [1][2][3][4]. The most promising area of DRS application is oncology [5] where it serves as a real-time and cost-effective diagnostics modality for differentiation of normal from pathological tissues [6][7][8][9], for determination of tumor oxygen status [10] and for monitoring the treatment process to predict outcomes [11].…”

“…Trans-illumination diffuse optical spectroscopy [10] based on the registration of photons passing through the tissue provides estimation of biotissue optical properties averaged over the entire tissue volume between source and detector, while in DRS the measurement volume is determined by the maximum reached depth of photons that return back to the surface. However, in contrast to trans-illumination techniques, DRS is more convenient in accessing tissues of different localizations, and, as a result, has a larger variety of biomedical applications including endoscopic ones.…”

Combined Monte Carlo and k-Wave Simulations for Reconstruction of Blood Oxygen Saturation in Optoacoustics: A Pilot Study

“…Optical imaging techniques [5,6] benefit from both non-invasiveness and high sensitivity to blood oxygenation owing to significant difference in absorption spectra of oxy-and deoxyhemoglobin in optical spectral range. Traditionally, blood oxygenation level is estimated with diffuse optical spectroscopy (DOS) [2,7,8] based on the registration of the spectra of the probing radiation passed through the biological tissue and the subsequent reconstruction of the medium absorption spectra from the measurement data [9,10]. This approach, however, provides with the estimation of blood oxygenation value averaged over a particular measurement volume within biological tissue.…”