Comparative study of three Marantodes pumilum varieties by microscopy, spectroscopy and chromatography

Aladdin, Nor Ashila; Jamal, Jamia Azdina; Talip, Noraini; Hamsani, Nur Ain M; Rahman, Mohd Ruzi A; Sabandar, Carla Wulandari; Muhammad, Kartiniwati; Husain, Khairana; Jalil, Juriyati

doi:10.1016/j.bjp.2015.10.002

Cited by 12 publications

(10 citation statements)

References 24 publications

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“…They are valuable for detecting the influence of climate on the petiole anatomical characteristics of designated organisms by comparing their characteristics with those of other areas (Allario et al, 2011). Taxonomists use various aspects linked with plant taxonomy to facilitate and develop the systematic identification, classification, and placement of plants (Aladdin et al, 2016; Araújo, Azevedo, Silva, & Meira, 2010). The petiole anatomy of plants was first carried out in Pakistan by Jehanzeb et al (2020), Kamel and Loutfy (2001), Khan, Sharif, Ahmad, Zafar, and Tareen (2009) exploring the epidermal cells, parenchyma, sclerenchyma, vascular bundles, subsidiary vascular bundles, trichomes shapes, and their dimensions.…”

Section: Introductionmentioning

confidence: 99%

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confidence: 99%

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Microscopic characterization of petiole anatomy of Asteraceous taxa of Western Himalaya‐Pakistan

Akhtar

Ahmad

Mahmood

et al. 2021

Microscopy Res & Technique

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Petiole anatomy of 15 species of family Asteraceae was examined which aimed to investigate petiolar anatomical structures for species level identification. Shandon Microtome was used for petiole histological preparations. Both qualitative and quantitative features were studied under microscope which showed significant variation in petiole, collenchyma, parenchyma shape/size, vascular bundles arrangement/size, and vessel elements quantity. Artemisia japonica Thunb., Cirsium vulgare (Savi) Ten., Myriactis nepalensis Less., Seriphidium brevifolium Ling & Y.R.Ling, Taraxacum officinale (L.) Weber ex F.H.Wigg., and Xanthium strumarium L. showed winged petioles. Maximum length and width of upper and lower epidermis was found in Tagetes erecta L. which is 23.05 ± 0.89 μm, 24.9 ± 1.257 μm length and 21.75 ± 1.38067 μm, 22.75 ± 0.467 μm width, respectively. Petioles of Parthenium hysterophorus L. was longest one with 9.85 ± 10.45 μm while A. japonica Thunb. showed highest number of vessel elements. Maximum size of vascular bundles was found in T. erecta L. with 5.05 ± 14.25 μm. Artemisia annua L., C. vulgare (Savi) Ten, Cyanthillium cinereum (L.) H.Rob., Helianthus annus L., M. nepalensis Less., P. hysterophorus L., Senecio chrysanthemoides DC. have trichomes while Tussilago farfara L. has highest number of vascular bundles. All species have angular collenchyma type except M. nepalensis Less., P. hysterophorus L., S. brevifolium Ling & Y.R.Ling, Tagetes minuta L., T. officinale L., S. chrysanthemoides DC., and T. farfara L. Cluster analysis implemented that distinct plant species in cluster. Petiolar anatomical structures and taxonomic key will helpful for distinguishing Asteraceous taxa at genus and species level. This taxonomic significant investigation will also provide baseline to taxonomists for other Asteraceae studies and phylogenetic research.

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Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

Microscopic characterization of petiole anatomy of Asteraceous taxa of Western Himalaya‐Pakistan

Akhtar

Ahmad

Mahmood

et al. 2021

Microscopy Res & Technique

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“…Microscopic examination enables primary species identification, as well as the detection of adulteration, contamination, and substitution of botanical drugs ( Upton et al, 2020 ) ( Table 3 ). Aladdin et al (2016) reported the first effective distinction of three M. pumilum varieties and plant parts utilizing microscopic approach. The microscopical characteristics, such as stomata, trichomes, stem and leaf margin, petiole, midrib, vascular system, anticlinal walls, secretory canals, and cell inclusion, can all be used to differentiate and identify each variety of M. pumilum and its plant part ( Table 2 ).…”

Section: Discussionmentioning

confidence: 99%

Authentication of Marantodes pumilum (Blume) Kuntze: A Systematic Review

et al. 2022

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Botanical drug products consist of complex phytochemical constituents that vary based on various factors that substantially produce different pharmacological activities and possible side effects. Marantodes pumilum (Blume) Kuntze (Primulaceae) is one of the most popular Malay traditional botanical drugs and widely recognized for its medicinal use. Many studies have been conducted focusing on the identification of bioactive substances, pharmacological and toxicological activities in its specific varieties but less comprehensive study on M. pumilum authentication. Lack of quality control (QC) measurement assessment may cause different quality issues on M. pumilum containing products like adulteration by pharmaceutical substances, substitution, contamination, misidentification with toxic plant species, which may be detrimental to consumers’ health and safety. This systematic literature review aims to provide an overview of the current scenario on the quality control of botanical drug products as determined by pharmacopoeia requirements specifically for M. pumilum authentication or identification. A systematic search for peer-reviewed publications to document literature search for M. pumilum authentication was performed using four electronic databases: Web of Science, PubMed, Scopus and ScienceDirect for related studies from January 2010 to December 2021. The research studies published in English and related articles for identification or authentication of M. pumilum were the main inclusion criteria in this review. A total 122 articles were identified, whereby 33 articles met the inclusion criteria. Macroscopy, microscopy, chemical fingerprinting techniques using chromatography, spectroscopy and hyphenated techniques, and genetic-based fingerprinting using DNA barcoding method have been used to identify M. pumilum and to distinguish between different varieties and plant parts. The study concluded that a combination of approaches is necessary for authenticating botanical drug substances and products containing M. pumilum to assure the quality, safety, and efficacy of marketed botanical drug products, particularly those with therapeutic claims.

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“…pumila is 4–15 cm long and slightly winged. Nonetheless, to differentiate them based on characteristic anatomical features and chemical profiling, a pharmacognostical study of these varieties was performed using microscopic, high-performance thin layer chromatography (HPTLC), high performance liquid chromatography (HPLC), and attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR) techniques [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

Xanthine oxidase inhibitory activity of a new isocoumarin obtained from Marantodes pumilum var. pumila leaves

Aladdin

Husain

Jalil

et al. 2020

BMC Complement Med Ther

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Background In traditional Malay medicine, Marantodes pumilum (Blume) Kuntze (family Primulaceae) is commonly used by women to treat parturition, flatulence, dysentery, dysmenorrhea, gonorrhea, and bone diseases. Preliminary screening of some Primulaceae species showed that they possess xanthine oxidase inhibitory activity. Thus, this study aimed to investigate the xanthine oxidase inhibitory activity of three varieties of M. pumilum and their phytochemical compounds. Method Dichloromethane, methanol, and water extracts of the leaves and roots of M. pumilum var. alata, M. pumilum var. pumila, and M. pumilum var. lanceolata were tested using an in vitro xanthine oxidase inhibitory assay. Bioassay-guided fractionation and isolation were carried out on the most active extract using chromatographic techniques. The structures of the isolated compounds were determined using spectroscopic techniques. Results The most active dichloromethane extract of M. pumilum var. pumila leaves (IC50 = 161.6 μg/mL) yielded one new compound, 3,7-dihydroxy-5-methoxy-4,8-dimethyl-isocoumarin (1), and five known compounds, viz. ardisiaquinone A (2), maesanin (3), stigmasterol (4), tetracosane (5), and margaric acid (6). The new compound was found to be the most active xanthine oxidase inhibitor with an IC50 value of 0.66 ± 0.01 μg/mL, which was not significantly different (p > 0.05) from that of the positive control, allopurinol (IC50 = 0.24 ± 0.00 μg/mL). Conclusion This study suggests that the new compound 3,7-dihydroxy-5-methoxy-4,8-dimethyl-isocoumarin (1), which was isolated from the dichloromethane extract of M. pumilum var. pumila leaves, could be a potential xanthine oxidase inhibitor.

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Comparative study of three Marantodes pumilum varieties by microscopy, spectroscopy and chromatography

Cited by 12 publications

References 24 publications

Microscopic characterization of petiole anatomy of Asteraceous taxa of Western Himalaya‐Pakistan

Microscopic characterization of petiole anatomy of Asteraceous taxa of Western Himalaya‐Pakistan

Authentication of Marantodes pumilum (Blume) Kuntze: A Systematic Review

Xanthine oxidase inhibitory activity of a new isocoumarin obtained from Marantodes pumilum var. pumila leaves

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