Comparative Study of the Pharmacological Properties and Biological Effects of Polygonum aviculare L. herba Extract-Entrapped Liposomes versus Quercetin-Entrapped Liposomes on Doxorubicin-Induced Toxicity on HUVECs

Abstract

This study aimed to evaluate the comparative biological effects of Polygonum aviculare L. herba (PAH) extract and quercetin-entrapped liposomes on doxorubicin (Doxo)-induced toxicity in HUVECs. HUVECs were treated with two formulations of liposomes loaded with PAH extract (L5 and L6) and two formulations of liposomes loaded with quercetin (L3 prepared with phosphatidylcholine and L4 prepared with phosphatidylserine). The results obtained with atomic force microscopy, zeta potential and entrapment liposome efficiency confirmed the interactions of the liposomes with PAH or free quercetin and a controlled release of flavonoids entrapped in all the liposomes. Doxo decreased the cell viability and induced oxidative stress, inflammation, DNA lesions and apoptosis in parallel with the activation of Nrf2 and NF-kB. Free quercetin, L3 and L4 inhibited the oxidative stress and inflammation and reduced apoptosis, particularly L3. Additionally, these compounds diminished the Nrf2 and NF-kB expressions and DNA lesions, principally L4. PAH extract, L5 and L6 exerted antioxidant and anti-inflammatory activities, reduced γH2AX formation and inhibited extrinsic apoptosis and transcription factors activation but to a lesser extent. The loading of quercetin in liposomes increased the cell viability and exerted better endothelial protection compared to free quercetin, especially L3. The liposomes with PAH extract had moderate efficiency, mainly due to the antioxidant and anti-inflammatory effects and the inhibition of extrinsic apoptosis.

Comparative Antioxidant and Antimicrobial Activities of Several Conifer Needles and Bark Extracts

“…Quercetin showed apoptotic effects, and quercetin, rutin, and chlorogenic acid decreased the symptoms of colitis. Catechin was studied for its apoptotic effects in breast, pancreatic, and colorectal cancers, and chlorogenic acid reduced neurodegeneration and provided neuroprotection against Parkinson's disease [40,41]. The antioxidant potential of stilbenes (i.e., resveratrol) was assessed by Reinisalo et al (2015), who revealed their therapeutic potential of reducing oxidative stress, thus decreasing the incidence of age-related diseases [42].…”

The genus Polygonum: An updated comprehensive review of its ethnomedicinal, phytochemical, pharmacological activities, toxicology, and phytopharmaceutical formulation

“…Over the past few decades, a number of liposomal medicinal items have been licensed and effectively utilized in clinics due to their benefits [ 206 ]. The purpose of the study by Mureşan et al [ 207 ] is to assess how the liposome made of P. aviculare extract and quercetin affects the doxorubicin-induced toxicity in human umbilical vein endothelial cells (HUVECs). Less so than quercetin and the liposomes containing quercetin, the P .…”

An integrative review of nonobvious puzzles of cellular and molecular cardiooncology

“…An integral element of cellular senescence and DOX-related toxicity is DNA damage, the spectrum of which is broad and includes DNA strand breaks, induction of DDR (γ-H2AX), and oxidative modifications [ 233 , 234 ]. A comparative analysis of DOX and daunorubicin concerning their ability to destroy EC DNA showed that both these analogs are genotoxic, albeit the effects caused by the latter are more pronounced [ 235 ].…”

“…A comparative analysis of DOX and daunorubicin concerning their ability to destroy EC DNA showed that both these analogs are genotoxic, albeit the effects caused by the latter are more pronounced [ 235 ]. DOX exerts its deleterious activity also towards cellular lipids, as evidenced according to increased concentration of malondialdehyde (MDA) in treated HUVECs [ 234 ].…”