Comparative study of the mucin-type sugar chains of human chorionic gonadotropin present in the urine of patients with trophoblastic diseases and healthy pregnant women.

Amano, Junko; Nishimura, R; Mochizuki, Matsuto; Kobata, Akira

doi:10.1016/s0021-9258(19)57280-5

Cited by 76 publications

(1 citation statement)

References 24 publications

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“…N-[3H]Acetylglucosaminitol and N-[t4C]acetylgalactosaminitol were prepared by NaBH4 reduction of the appropriate radiolabeled reducing sugars and were purified after reduction by passage over a column of mixed bed ion exchange resin in water. The disaccharide standard [3H]GaI-131,4GIcNAc was prepared enzymatically from GIcNAc (Sigma Chemical Co.) using bovine galactosyltransferase (UDP-galactose/D-glucose-4-galactosyl-transferase, Sigma Chemical Co.) and UDP-[6-3H]galactose (Amano et al, 1988). The reduced form of this disaccharide was prepared by reduction with NaBH4.…”

Section: Reagentsmentioning

confidence: 99%

Immunocytochemical localization of mutant low density lipoprotein receptors that fail to reach the Golgi complex.

Pathak

Merkle

Cummings

et al. 1988

The Journal of Cell Biology

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Abstract. In the low density lipoprotein (LDL) receptor system, blocks in intracellular movement of a cell surface receptor result from naturally occurring mutations. These mutations occur in patients with familial hypercholesterolemia. One class of mutant LDL receptor genes (class 2 mutations) produces a receptor that is synthesized and glycosylated in the endoplasmic reticulum (ER) but does not reach the cell surface. These receptors contain serine/threonine-linked (O-linked) carbohydrate chains with core N-acetylgalactosamine residues and asparagine-linked (N-linked) carbohydrate chains of the high mannose type that are only partially trimmed. To determine the site of blockage in transport, we used electron microscope immunohistochemistry to compare the intracellular location of LDL receptors in normal human fibroblasts with their location in class 2 mutant fibroblasts. In normal cells, LDL receptors were located in coated pits, coated vesicles, endosomes, multivesicular bodies, and portions of the Golgi complex, In contrast, the mutant receptors in class 2 cells were almost entirely confined to rough ER and irregular extensions of the rough ER. Metabolic labeling studies with [3H]glucosamine confirmed that these mutant receptors contain core O-linked sugars, suggesting that the enzymes that attach these residues are located in the rough ER or the transitional zone of the ER. These studies establish that naturally occurring mutations in cell surface receptors can cause the receptors to remain trapped in the ER, thereby preventing their normal function and producing a genetic disease.

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Section: Reagentsmentioning

confidence: 99%

Immunocytochemical localization of mutant low density lipoprotein receptors that fail to reach the Golgi complex.

Pathak

Merkle

Cummings

et al. 1988

The Journal of Cell Biology

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Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update covering the period 2001–2002

Harvey¹

2008

Mass Spectrometry Reviews

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This review is the second update of the original review on the application of MALDI mass spectrometry to the analysis of carbohydrates and glycoconjugates that was published in 1999. It covers fundamental aspects of the technique as applied to carbohydrates, fragmentation of carbohydrates, studies of specific carbohydrate types such as those from plant cell walls and those attached to proteins and lipids, studies of glycosyl-transferases and glycosidases, and studies where MALDI has been used to monitor products of chemical synthesis. Use of the technique shows a steady annual increase at the expense of older techniques such as FAB. There is an increasing emphasis on its use for examination of biological systems rather than on studies of fundamental aspects and method development and this is reflected by much of the work on applications appearing in tabular form.

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Hyperglycosylated hCG cannot be measured using a sialic acid–specific lectin immunoassay

Cole

2003

Prenatal Diagnosis

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Comparative study of the mucin-type sugar chains of human chorionic gonadotropin present in the urine of patients with trophoblastic diseases and healthy pregnant women.

Cited by 76 publications

References 24 publications

Immunocytochemical localization of mutant low density lipoprotein receptors that fail to reach the Golgi complex.

Immunocytochemical localization of mutant low density lipoprotein receptors that fail to reach the Golgi complex.

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update covering the period 2001–2002

Hyperglycosylated hCG cannot be measured using a sialic acid–specific lectin immunoassay

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