Comparative study of the genepath group 4 reagent system and other chef systems for karyotype analysis of <i>candida</i> spp

Holmberg, K.; Feroze, Farhana

doi:10.1002/jcla.1860090307

Cited by 11 publications

(6 citation statements)

References 35 publications

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“…The U.S. and European isolates were identified as described previously (2,11,12). Singaporean isolates were identified as C. albicans by an API 20C test (API, bioMerieux, Marcy l'Etoile, France) and a positive germ tube test (3,4).…”

Section: Methodsmentioning

confidence: 99%

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Comparative analysis of genetic variability among Candida albicans isolates from different geographic locales by three genotypic methods

et al. 1997

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The objective of the present study was to conduct a comparative genotypic analysis of Candida albicans isolates from the United States, Europe, and Southeast Asia to determine whether differences between isolates might be associated with geographic locations. The genotypes of 86 unrelated isolates of C. albicans (from the United States and Europe) and 26 isolates from Singapore were examined by three DNA typing methods. Computer-assisted methods were used to analyze the gel patterns for all isolates. A dendrogram based on the overall similarity of the patterns obtained by restriction endonuclease analysis (REA) with EcoRI clustered the U.S. and European isolates into two major groups (groups A and B). The Singaporean isolates demonstrated unique REA profiles, with nine isolates having both or neither of the REA-characteristic 3.7-and 4.2-kb bands present in groups A and B. By REA profiles, the Singaporean isolates were related to each other with similarity values (S AB s) of >0.80, but only one isolate mixed with the U.S. and European isolates at this S AB (an arbitrary threshold for genetic similarity). Randomly amplified polymorphic DNA (RAPD) analysis generated DNA profiles that clustered the C. albicans isolates into approximately the same number of distinct typing groups as REA. However, isolates identical to each other by REA were generally different from each other by RAPD analysis. In a composite dendrogram prepared from the results obtained by RAPD analysis, the isolates from the United States and Europe clustered in major groups with S AB s of >0.85, while Singaporean isolates connected to these clusters at S AB s of >0.75. Pulsed-field gel electrophoresis was less discriminatory, discerning about one-third as many distinct subtypes as REA or RAPD analysis; the Singaporean isolates were distributed randomly with the U.S. and European isolates. These results suggest that a high degree of genetic diversity exists between C. albicans isolates from Southeast Asia and those from the United States and Europe.

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Section: Methodsmentioning

confidence: 99%

“…PFGE method. Yeast chromosomal DNA for PFGE was isolated as described previously (3). Chromosomal separations were done by contour-clamped homogeneous electric field (CHEF) electrophoresis with a CHEF-MAPPER system (Bio-Rad, Hercules, Calif.).…”

Section: Methodsmentioning

confidence: 99%

Comparative analysis of genetic variability among Candida albicans isolates from different geographic locales by three genotypic methods

et al. 1997

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“…Before DNA-testing the identity of the strains was confirmed by the analytical profile index procedureAPI2OC (API, bioMerieux, Marcy-l'Etoile, France), germ tube test and chlamydospore formation, and from the basic pattern of the karyotype (number and approximate size of chromosomes) generated in pulsed-field gel electrophoresis of genomic DNA according a method described previously (32).…”

Section: Organismsmentioning

confidence: 99%

“…Yeast chromosomal DNAfor PFGE was prepared and separated by contour-clamped homogenous electric (CHEF) electrophoresis using the CHEF-Mapper system (Bio-Rad, Hercules, CA) as described previously (32).…”

Section: Pfgementioning

confidence: 99%

Evaluation of an optimized system for random amplified polymorphic DNA (RAPD)-analysis for genotypic mapping ofCandida albicans strains

Holmberg¹,

Feroze²

1996

J. Clin. Lab. Anal.

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“…Organismos com elevada variabilidade genética podem expressar fenótipos altamente variáveis e, portanto, ocultar diferenças inter ou intra-específicas. Essas evidências surgem principalmente com diferentes enzimas não-metabólicas que exibem altas variações estruturais em virtude da intensidade da pressão seletiva ambiental (59,60,61,62,63). Ao contrário, enzimas metabólicas apresentam baixa vulnerabilidade à seleção ambiental e usualmente são empregadas como marcadores isoenzimáticos (64).…”

Section: Introductionunclassified

Ferramentas Moleculares Para Caracterização De Candida Albicans (Robin) Berkhout (1923) Em Estudos Epidemiológicos

Boriollo¹,

Höfling

Mendes

et al. 2005

Estud Biol.

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Essa revisão busca apresentar uma descrição dos principais recursos moleculares empregados em estudos epidemioló gicos envolvendo candidoses. São abordados os recursos mais comumente empregados em Epidemiologia Molecular e baseados em polimorfismos proté icos (Eletroforese de Isoenzimas) e de á cidos nuclé icos (Eletrocariotipagem, Polimorfismo dos Fragmentos de Restrição, e Polimorfismos do DNA Amplificado ao Acaso). São apresentadas as características de cada mé todo, bem como suas vantagens e desvantagens.

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Comparative study of the genepath group 4 reagent system and other chef systems for karyotype analysis of candida spp

Cited by 11 publications

References 35 publications

Comparative analysis of genetic variability among Candida albicans isolates from different geographic locales by three genotypic methods

Comparative analysis of genetic variability among Candida albicans isolates from different geographic locales by three genotypic methods

Evaluation of an optimized system for random amplified polymorphic DNA (RAPD)-analysis for genotypic mapping ofCandida albicans strains

Ferramentas Moleculares Para Caracterização De Candida Albicans (Robin) Berkhout (1923) Em Estudos Epidemiológicos

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