Evaluation of an optimized system for random amplified polymorphic DNA (RAPD)-analysis for genotypic mapping ofCandida albicans strains

Value of morphotyping for the characterization of Candida albicans clinical isolates

“…In accordance with previous reports (Bostock et al 1993, Schonian et al 1993, Holmberg & Feroze 1996, Clemons et al 1997, Pujol et al 1997, RAPD analysis confirmed to be highly discriminatory and suitable for largescale epidemiological studies of C. albicans as well as for inquiries in epidemic outbreaks. In laboratories not equipped for molecular methods, isolates should be sent to reference laboratories for epidemiological studies.…”

Mycotic keratitis: an overview of diagnosis and therapy

“…Polymerase chain reaction has emerged as a new promising tool, which has been shown to be useful for the culture-independent diagnosis of various microbial infections, including mycoses. [105][106][107][108] This method has been used for the diagnosis of keratitis in rabbit model of Fusarium keratitis using specific primers. 109 As described above, there are more than 56 fungal genera known to cause mycotic keratitis, thus, there is a need to design fungal specific primers, which anneal only with fungal DNA but not with other microbes including bacteria, viruses and Acanthamoeba DNA that are other most common causal agents of keratitis.…”

The Ins and Outs of DNA Fingerprinting the Infectious Fungi

“…The RAPD method of DNA fingerprinting has become quite popular for all infectious fungi and has been successfully applied to C. albicans (see, e.g., references 22,36,37,77,83,86,131,140,141,181,223,288,306,309,369, and 393), C. dubliniensis (79), C. parapsilosis (199), C. lusitaniae (163,181), C. tropicalis (181,187), C. glabrata (195,181,337,387), C. krusei (437), C. famata (437), C. rugosa, (437), A. fumigatus (5,11,178,187,200,202,228,296), A. flavus (51), Cryptococcus neoformans (32,33,41,68,71,72,117,197,282,311,…”