Comparative study of the effects of osteoprotegerin and testosterone on bone quality in male orchidectomised rats

Abstract

ORX resulted in femoral and vertebral bone loss and in microarchitectural deterioration. Treatment with OPG-Fc and testosterone recovered lumbar (L) and femoral (F) bone mineral densitometry bone mineral density (BMD) to SHAM levels. Femoral BMD was significantly higher after treatment with OPG-Fc than after testosterone treatment due to the presence of osteopetrotic changes in the metaphyseal region of long bones. Serum levels of ALP and CTX increased, while OPG levels were unchanged in ORX rats. Treatment with OPG-Fc decreased the levels of BGP, ALP, and CTX. Treatment with testosterone maintained biochemical markers of bone turnover at levels similar to or higher than those of ORX rats.

Physiological testosterone replacement effects on male aged rats with orchiectomy-induced osteoporosis in advanced stage: a tomographic and biomechanical pilot study

“…The available evidence regarding the testosterone role in bone health has been described in different methods, and despite the fact that the results demonstrate that the hormone absence is related to a decrease in the bone quality, the information reporting the effect of testosterone therapy on the improvement of bone parameters are still scarce [13][14][15][16][17][18][19]. In our study, we evaluated the effect of physiological testosterone replacement on male aged rats with advanced stage of osteoporosis induced by physical castration.…”

“…Therefore, it seems plausible to correlate low bioavailable testosterone levels in aging men with loss in mineral bone density (BMD), bone volume and increased fracture risk, similar to women in menopause. The clinical or in vivo literature regarding low bioavailable testosterone and hormonal replacement in correlation with bone loss and increased risk of fracture in men is still limited [13][14][15][16][17][18][19]. Although there are some clinical trials showing an improvement of BMD by testosterone replacement treatment, Lee et al [15] described that most studies evaluating testosterone replacement in hypogonadal men have been uncontrolled, performed in a short term and involving a small sample size with a variety of causes for their hypogonadism.…”

Differential Modulation of Cancellous and Cortical Distal Femur by Fructose and Natural Mineral-Rich Water Consumption in Ovariectomized Female Sprague Dawley Rats

“…For analysis of cortical bone, the VOI began at a distance of 7 mm from the growth plate, and it extended 2 mm in the proximal direction, excluding cancellous bone (Figure S1). The selection of the corresponding VOIs, and consequent bone microstructural properties, and vBMD analyses were carried out with commercial software provided with the micro-CT equipment (SkyScan CT-Analyser; version 1.10.0.2) [49]. Bone measurements were obtained by staff who were blinded to the treatment group of the rats.…”

“…Structure model index (SMI), which indicates the relative prevalence of rods and plates in the 3D structure, and trabecular bone pattern factor (Tb.Pf, mm −1 ), which shows the connectedness of a cancellous bone structure described by the relation of convex to concave surfaces of the trabeculae, which were also calculated using the direct 3D model. Degree of anisotropy (DA) represents trabecular anisotropy defined as the ratio between the maximal and minimal radius of the mean intercept length [49].…”

“…Parameters obtained by 3D analysis included cortical thickness (Ct.Th, µm), cortical volume (Ct.V, mm 3 ), and medullary volume (MV, mm 3 ). Parameters obtained by 2D analysis included cross-sectional thickness (Cs.Th; µm; an alternative measure of cortical thickness); mean total cross-sectional bone area (B.Ar, mm 2 ); cortical endosteal perimeter (Ct.En.Pm, mm); cortical periosteal perimeter (Ct.Pe.Pm, mm); mean polar moment of inertia (polar, MMI, mm 4 ), which denotes the resistance to rotation of a cross-section about a chosen axis; and mean eccentricity (Ecc), a parameter that shows the difference between the elongation of the cortical region (generally elliptical in shape) with respect to a circular shape [49].…”