Comparative study of the application of microcurrent and AsGa 904 nm laser radiation in the process of repair after calvaria bone excision in rats

Mendonça, Josué Sampaio; Neves, Lia Mara Grosso; Esquisatto, Marcelo Augusto Marretto; Mendonça, Fernanda Aparecida Sampaio; Santos, Gláucia Maria Tech dos

doi:10.1088/1054-660x/23/3/035605

Cited by 9 publications

(20 citation statements)

References 49 publications

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“…A 5.0 mm saw (SIN 300.0112—50W/100 Hz) under constant irrigation of sterile physiological solution was used to create a critical defect of 25 mm 2 in calvaria preserving the dura mater. After the surgical procedure, analgesia with sodium dipyrone (500 mg/mL) and tramadol (0.1 mg/kg) was used in the water for 72 hr …”

Section: Methodsmentioning

confidence: 99%

“…The microcurrent application was performed using a low‐intensity transcutaneous electrical stimulator (Physiotonus Microcurrent, BIOSET ® , Indústria de Tecnologia Eletrônica Ltd., Rio Claro, SP, Brazil). Two metal electrodes were placed around the bone defect (10 μA/05 min) . The applications started after surgery and were performed on alternate days during the experimental period.…”

Section: Methodsmentioning

confidence: 99%

“…Osteogenesis induced by electrical stimulation was initially described by Yasuda (1953) . Since then, it has been demonstrated that the exogenous application of electric currents at physiological levels play a role in cellular and molecular signaling pathways, stimulating the synthesis, and release of cytokines, growth factors, proteoglycans, proliferation, and differentiation of osteogenic cells . In addition, the application of low‐intensity electric current (microcurrent) activates calcium transport through its voltage‐dependent channels .…”

Section: Introductionmentioning

confidence: 99%

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Electrical stimulation: Complementary therapy to improve the performance of grafts in bone defects?

et al. 2018

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The limitations of bone reconstruction techniques have stimulated the tissue engineering for the repair of large bone defects using osteoconductive materials and osteoinductive agents. This study evaluated the effects of low intensity electric current on the inorganic bovine graft in calvaria defects. Bone defects were performed with piezoelectric system in the calvaria of Wistar rats divided into four groups (n = 24): (C) without grafting and without electrical stimulation; (E) with grafting; (MC) without grafting and submitted to electrical stimulation; (MC + E) with grafting and submitted to electrical stimulation. Inflammatory, angiogenic and osteogenic events during bone repair at the 10th, 30th, 60th, and 90th days were considered. Several inflammatory markers demonstrated the efficacy of grafting in reducing inflammation, particularly when subjected to electrical stimulation. Angiogenesis and collagen organization were more evident by electrical stimulation application on the grafts. Moreover, the osteogenic cell differentiation process indicated that the application of microcurrent on grafting modulated the homeostasis of bone remodeling. It is concluded that microcurrent favored the performance of grafts in calvarial rat model. Low-intensity electrical current might improve the osteoconductive property of grafting in bone defects. Therefore, electrical current becomes an option as complementary therapy in clinical trials involving bone surgeries and injuries.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Electrical stimulation: Complementary therapy to improve the performance of grafts in bone defects?

et al. 2018

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“…For this purpose, the animals were immobilised without sedation and treated daily at the same time for 21 days. Daily treatment was chosen according to the literature and experimental designs previously conducted by our and other research groups [15,17,24,51,52].…”

Section: Methodsmentioning

confidence: 99%

“…The density energy used in this experimental model has been used by our research group with favourable results for tissue repair [15,51,52], and the literature reports that different authors used similar energy density [13,24,25,53]. Non-contact laser irradiation was performed punctually, in the continuous mode, at a distance of ±2 mm and an angle of 90°in relation to the wound surface.…”

Section: Methodsmentioning

confidence: 99%

InGaP 670-nm laser therapy combined with a hydroalcoholic extract of Solidago chilensis Meyen in burn injuries

Catarino¹,

Godoy²,

Scharlack³

et al. 2015

Lasers Med Sci

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Therapies that accelerate the healing of burn injuries, improving the quality of life of the patient and reducing the cost of treatment are important. This study evaluated the effects of InGaP 670-nm laser therapy combined with a hydroalcoholic extract of Solidago chilensis leaves on burn wound healing in rats. Seventy-two rats were divided randomly into four groups: control untreated (C), treated with InGaP 670-nm laser with power density of 0.41 W/cm(2) and energy density of 4.93 J/cm(2) (L), treated with S. chilensis extract (S) and treated with S. chilensis extract and laser (LS). Second-degree burns were produced on the back of the animals with metal plate. Wound samples were collected on days 7, 14 and 21 of treatment for structural analysis, morphometry and Western blotting to quantify the expression of transforming growth factor beta 1 (TGF-β1) and vascular endothelial growth factor (VEGF). The results showed that InGaP laser irradiation at 670 nm alone and combined with extract of S. chilensis promoted significant tissue repair responses in this experimental model, increasing the number of fibroblasts, collagen fibres and newly formed blood vessels throughout the experimental period and decreasing the number of granulocytes in burn wounds of second degree in all treated groups. Exclusive treatment of burn wounds with the hydroalcoholic extract of S. chilensis provided similar quantitative results to those seen in the untreated group throughout the experimental period. Therefore, it was observed in the L and LS groups different responses in the expression of TGF-β1 and VEGF. The application of 670-nm laser alone or combined with the extract of S. chilensis promoted favourable responses in tissue repair of second-degree burns in this experimental model.

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Electrical stimulation therapy and rotary jet‐spinning scaffold to treat bone defects

Santos

Aro

2022

The Anatomical Record

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The combination of electrical stimulation (ES) and bone tissue engineering (BTE) has been successful in treatments of bone regeneration. This study evaluated the effects of ES combined with PCL + β-TCP 5% scaffolds obtained by rotary jet spinning (RJS) in the regeneration of bone defects in the calvaria of Wistar rats. We used 120 animals with induced bone defects divided into 4 groups (n = 30): (C) without treatment; (S) with PCL+ β-TCP 5% scaffold; (ES) treated with ES (10 μA/5 min); (ES + S) with PCL + β-TCP 5% scaffold. The ES occurred twice a week during the entire experimental period. Cell viability (in vitro: Days 3 and 7) and histomorphometric, histochemical, and immunohistochemical (in vivo; Days 30, 60, and 90) analysis were performed.In vitro, ES + S increased cell viability after Day 7 of incubation. In vivo, it was observed modulation of inflammatory cells in ES therapy, which also promoted blood vessels proliferation, and increase of collagen. Moreover, ES therapy played a role in osteogenesis by decreasing ligand kappa B nuclear factor-TNFSF11 (RANKL), increasing alkaline phosphatase (ALP), and decreasing the tartarate-resistant acid phosphatase. The combination of ES with RJS scaffolds may be a promising strategy for bone defects regeneration, since the therapy controlled inflammation, favored blood vessels proliferation, and osteogenesis, which are important processes in bone remodeling.

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Comparative study of the application of microcurrent and AsGa 904 nm laser radiation in the process of repair after calvaria bone excision in rats

Cited by 9 publications

References 49 publications

Electrical stimulation: Complementary therapy to improve the performance of grafts in bone defects?

Electrical stimulation: Complementary therapy to improve the performance of grafts in bone defects?

InGaP 670-nm laser therapy combined with a hydroalcoholic extract of Solidago chilensis Meyen in burn injuries

Electrical stimulation therapy and rotary jet‐spinning scaffold to treat bone defects

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