Comparative study of the antifungal activity of some essential oils and their major phenolic components against Aspergillus niger using three different methods

Bouddine, Latifa; Louaste, Bouchra; Achahbar, Sanaa; Chami, Najat; Chami, Fouzia; Remmal, Adnane

doi:10.5897/ajb11.3293

Cited by 29 publications

(13 citation statements)

References 28 publications

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“…It was also observed that the thyme EOs used belongs to the thymol chemotype. Thymol is generally recognized as a safe food and many publications have reported thymol had better activity than carvacrol (Bouddine et al 2012). Solomakosa et al (2008) showed that the combined addition of thyme EOs at 0.6 % and nisin at 500 or 1000 IU/g showed a synergistic activity against L. monocytogenes.…”

Section: Discussionmentioning

confidence: 99%

Enhanced bactericidal effect of enterocin A in combination with thyme essential oils against L. monocytogenes and E. coli O157:H7

Ghrairi

Hani

2013

J Food Sci Technol

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The combined effects of enterocin A with Thymus vulgaris essential oils (EOs) against Listeria monocytogenes and Escherichia coli O157:H7 were investigated in vitro by enumeration of surviving populations of testing pathogens and minimal inhibitory concentration (MIC) determination. Enterocin A was purified to homogeneity by RP-HPLC from the culture fluid of Enterococcus strain and thyme EOs were extracted from local Thymus vulgaris plants. The major constituent of thyme EOs oils determined by GC-MS was thymol (78.4 %). Combination of enterocin A with thyme EOs showed an enhanced bactericidal effect against Listeria monocytogenes. Checkerboard assay and isobologram construction displayed a synergistic interaction between these compounds against Listeria (FIC index <0.5). Moreover, the MIC value of enterocin A has fallen fivefold (from 4.57 to 0.9 μg/ml), while the MIC of thyme EOs decreased threefold (from 3.6 to 1.2 μg/ml). Treatments with enterocin A alone did not affect the growth of the enteric pathogen E. coli O157:H7. However, the addition of thyme EOs and enterocin A yielded a synergistic antimicrobial effect against E. coli (MIC thyme EOs decrease from 2.2 to 0.71 μg/ml). This is the first report on the combined effect of enterocin A and thyme EOs against food pathogen bacteria. This combination could be useful in food bio-preservation.

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Section: Discussionmentioning

confidence: 99%

Enhanced bactericidal effect of enterocin A in combination with thyme essential oils against L. monocytogenes and E. coli O157:H7

Ghrairi

Hani

2013

J Food Sci Technol

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“…Furthermore, thymol, carvacrol, and eugenol have also been demonstrated to yield antifungal activity against Aspergillus sp. [ 121 ]. Phenolic compounds are also well known for their antiparasitic activity.…”

Section: Targeting Polymicrobial Diseases: Current and Promising Amentioning

confidence: 99%

Current State and Promising Opportunities on Pharmaceutical Approaches in the Treatment of Polymicrobial Diseases

et al. 2021

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In recent years, the emergence of newly identified acute and chronic infectious disorders caused by diverse combinations of pathogens, termed polymicrobial diseases, has had catastrophic consequences for humans. Antimicrobial agents have been clinically proven to be effective in the pharmacological treatment of polymicrobial diseases. Unfortunately, an increasing trend in the emergence of multi-drug-resistant pathogens and limited options for delivery of antimicrobial drugs might seriously impact humans’ efforts to combat polymicrobial diseases in the coming decades. New antimicrobial agents with novel mechanism(s) of action and new pharmaceutical formulations or delivery systems to target infected sites are urgently required. In this review, we discuss the prospective use of novel antimicrobial compounds isolated from natural products to treat polymicrobial infections, mainly via mechanisms related to inhibition of biofilm formation. Drug-delivery systems developed to deliver antimicrobial compounds to both intracellular and extracellular pathogens are discussed. We further discuss the effectiveness of several biofilm-targeted delivery strategies to eliminate polymicrobial biofilms. At the end, we review the applications and promising opportunities for various drug-delivery systems, when compared to conventional antimicrobial therapy, as a pharmacological means to treat polymicrobial diseases.

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“…Syzygium aromaticum (clove) is an aromatic medicinal plant that belongs to the Myrtaceae family [19]. Reportedly, clove oil possesses antifungal, antibacterial, antiviral, and insecticidal properties due to the presence of phytoactive compounds such as eugenol, eugenyl acetate, and β-caryophyllene [19][20][21]. The antifungal activity of S. aromaticum has been demonstrated by Khan et al (2009), who reported that the ethanolic extract of clove was highly effective against C. albicans with a Minimum inhibitory concentration (MIC) value at 156 μg/mL [22].…”

Section: Introductionmentioning

confidence: 99%

In vitro anticandidal potency of Syzygium aromaticum (clove) extracts against vaginal candidiasis

Yassin

Mostafa

Al-Askar

2020

BMC Complement Med Ther

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Background: Candida vaginitis is a global health hazard that increases morbidity among women of childbearing age. Recent studies have revealed a high incidence of drug-resistant Candida strains. Additionally, treating Candida vulvovaginitis during pregnancy is challenging as antifungal therapy is associated with fetal abnormalities. Hence, it is important to develop novel therapeutic strategies to treat vulvovaginal candidiasis. Methods: In this study, we used the disc diffusion method to evaluate the anticandidal activity of different Syzygium aromaticum extracts (methanol, ethyl acetate, n-hexane, and diethyl ether) against C. albicans, C. glabrata, and C. tropicalis. Furthermore, gas chromatography-mass spectrometry (GC-MS) analysis of different S. aromaticum extracts was performed to determine active components exhibiting anticandidal activity. Cytotoxicity of different clove extracts against the HUH7 cell line was evaluated. Results: The ethyl acetate extract exhibited the highest antifungal activity against C. albicans, C. glabrata, and C. tropicalis with inhibition zone diameters of 20.9, 14.9, and 30.7 mm, respectively. The minimum inhibitory concentration of the S. aromaticum ethyl acetate extract was 250 μg/disc against C. tropicalis, and 500 μg/disc against C. albicans and C. glabrata, while the minimum fungicidal concentration was 0.5 mg/disc against C. tropicalis and 1 mg/disc against the C. albicans and C. glabrata. GC-MS analysis of the ethyl acetate extract revealed the main bioactive compound as eugenol (58.88%), followed by eugenyl acetate (23.86%), transcaryophyllene (14.44%), and α-humulene (1.88%). The cytotoxicity assay indicated that the diethyl ether extract demonstrated the lowest toxicological effect against the HUH7 cell line, with a relative IC 50 of 62.43 μg/ml; the methanolic extract demonstrated a higher toxicity (IC 50 , 24.17 μg/ml). Conclusion: As the S. aromaticum extract exhibited high antifungal activity at low concentrations, it can be a potential source of natural antifungal drugs.

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Comparative study of the antifungal activity of some essential oils and their major phenolic components against Aspergillus niger using three different methods

Cited by 29 publications

References 28 publications

Enhanced bactericidal effect of enterocin A in combination with thyme essential oils against L. monocytogenes and E. coli O157:H7

Enhanced bactericidal effect of enterocin A in combination with thyme essential oils against L. monocytogenes and E. coli O157:H7

Current State and Promising Opportunities on Pharmaceutical Approaches in the Treatment of Polymicrobial Diseases

In vitro anticandidal potency of Syzygium aromaticum (clove) extracts against vaginal candidiasis

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