Comparative study of the anti-inflammatory, antioxidant and urease inhibitory activities of Eryngium kotschyi Boiss. and E. campestre L. var. virens (Link) Weins extracts

Abstract

Despite widespread traditional usage of Eryngium species in Anatolia (Turkey), only a limited number of scientific studies exists on E. kotschyi, an endemic species. Previously, extracts from E. campestre and E. kotschyi were reported to have significant anti-inflammatory effects in vivo. This study aimed to investigate the in vitro antiinflammatory, antioxidant, urease inhibitory activities of ethanol extracts of E. kotschyi and E. campestre var. virens roots as well as distilled water and ethanol extracts of E. kotschyi aerial parts. The NO and cytokine inhibitory effects were evaluated by Griess and ELISA assays. The antioxidant activities were tested on DPPH • , ABTS •+ and CUPRAC assays. The EtOH extract of E. kotschyi roots (EKr EtOH) and aerial parts (EKae EtOH) inhibited 50.08% and 41.52% of NO production at 100 µg/ml, respectively. The EtOH extract of the roots of E. campestre var. virens (ECr EtOH) and EKr EtOH provided 36.22% and 65.23% IL-6 inhibition and 44.24% and 56.84% IL-1α inhibition at 100 µg/ml. EKae EtOH exerted highest antioxidant activity on ABTS •+ (2.4±0.0005 µM trolox/mg extract) and CUPRAC (0.97±0.07 mM trolox/mg extract). This extract was also found the richest among all in terms of phenolics content (6.1±0.001 mg/GAE/g extract). EKr EtOH and ECr EtOH exhibited strongest DPPH • (IC50 = 1.132±0.057 mg/ml) radical scavenging and ferric reducing/antioxidant power (0.36±0.005 mM Fe2+/mg extract) activity respectively. The extracts exerted low urease inhibitory activity. Consequently, the results of this study might contribute to the elucidation of the mechanism of in vivo anti-inflammatory activity of the extracts.

Characterization of antioxidant properties of strawberry tree (Arbutus unedo L.) and trace elements determination

“…There are more than 20 methods given in the literature for the measurement of antioxidant capacity. In the case of determining the antioxidant capacities of plants and the total phenolic content of the plant extracts, the results in the literature clearly show that antioxidant activity is highly dependent on the chosen method of determination and a complete correlation may not be observed between the observed antioxidant activity (or capacity) [30,31,32].…”