Comparative study of some characteristics of the semen of RR (rose comb) or rr (single comb) cockerels

Petitjean, M.; Servouse, Madeleine; Malineau, G.; Marchand, M. J.

doi:10.1051/rnd:19810807

Cited by 9 publications

(8 citation statements)

References 7 publications

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“…Buckland et al (1969) reported a positive correlation between fumarase activity and fowl spermatozoal fertilizing ability, reporting that spermatozoa from RR males were characterized with reduced fumarase activity. However, Petitjean and Servouse (1981) observed no difference in fumarase activity when spermatozoa from RR and rr males were compared. Likewise, although Crawford and Smith (1964d) observed no difference in metabolic rate among spermatozoa from RR, Rr, and rr males, as evidenced by methylene blue reduction time, Kirby and Froman (1991a) reported that the metabolic capacity of RR males was only 63% of that of rr males as evidenced by the reduction of 2-(p-iodophenyl)-3-(p-nitrophenyl)-5phenyl tetrazolium chloride (INT) to formazan.…”

Section: Introductionmentioning

confidence: 60%

“…They reported no differences in sperm morphology, viability, or testicular attributes that could account for subfertility. Although Buckland et al (1969) reported that spermatozoa from homozygotes were deficient in fumarase activity, Petitjean and Servouse (1981) were unable to identify any genotypic effect on spermatozoal fumarase activity.…”

Section: Discussionmentioning

confidence: 94%

“…Although some authors, notably Petitjean and Cochez (1966) as well as Petitjean and Servouse (1981), have implicated poor spermatozoal motility as a contributing factor, the mechanism responsible for spermatozoal dysfunction is unknown. However, heritable subfertility of RR males cannot be attributed to spermatozoal degeneration as reported for subfertile Delaware roosters (Froman and Bernier, 1987;Kirby et al, 1989).…”

Section: Introductionmentioning

confidence: 97%

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Analysis of Subfertility Associated with Homozygosity of the Rose Comb Allele in the Male Domestic Fowl

1994

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Sperm metabolism and duration of fertility were compared between roosters homozygous (RR) and heterozygous (Rr) for the rose comb allele. In the absence or presence of Ca+2, a motility agonist, the metabolic rate of spermatozoa from RR males was 76 to 77% of that of spermatozoa from Rr males. In the presence of caffeine, the metabolic rate of spermatozoa from RR males was increased significantly (P < .05) to 86% of that of spermatozoa from Rr males. Although intramagnal insemination of ejaculated spermatozoa reduced the disparity in duration of fertility between RR and Rr males, the duration of fertility of RR males was still less than that of Rr males. No difference in duration of fertility was observed following intramagnal insemination of testicular spermatozoa. Subfertility associated with RR males seems to stem from an aberrant activation or regulation of spermatozoal metabolism and not a reduction in absolute metabolic capacity per se.

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Section: Introductionmentioning

confidence: 60%

Section: Discussionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 97%

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Analysis of Subfertility Associated with Homozygosity of the Rose Comb Allele in the Male Domestic Fowl

1994

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“…subfertile due to sperm death or degeneration as the result of a single dominant gene (Froman & Bernier, 1987;Kirby et ai, 1989Kirby et ai, , 1990a whereas spermatozoa from RR roosters are characterized by poor motility (Buckland et ai, 1969;Etches et ai, 1974;Petitjean & Servouse, 1981). The possi¬ bility that the decreased life span of Delaware spermatozoa could be due to increased metabolism merited further consideration.…”

Section: Discussionmentioning

confidence: 99%

Comparative metabolism of spermatozoa from subfertile Delaware and Wyandotte roosters

Kirby¹,

Froman²

1991

Reproduction

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Sperm metabolism was determined via reduction of 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyl tetrazolium chloride (INT) to formazan. When the reaction mixture contained cyanide, which blocks cytochrome oxidase and thus maximizes intermediate electron transfer to INT, and calcium which stimulates fowl sperm motility, the metabolic capacity of spermatozoa from subfertile Delaware and Wyandotte roosters was 90 and 63% of that of spermatozoa from fertile Leghorn roosters. When the assay was performed at 40 degrees C without calcium or cyanide, no difference in metabolism was observed between Delaware and Leghorn spermatozoa (P greater than 0.05). However, the metabolism of Wyandotte spermatozoa was 66% of that observed with Delaware or Leghorn spermatozoa. These results provide further evidence that heritable subfertility in Delaware and Wyandotte roosters is attributable to distinct sperm defects.

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“…Most notably, fumarase activity was reported to be less in sperm from R/R males than in that from controls, and the activity of this enzyme was correlated to fertility [13]. However, this finding was later contradicted [14]. Kirby et al [4] and McLean and Froman [8] used a test of overall metabolic activity to show that metabolism of sperm from R/R males was less than for controls.…”

Section: Corvallis Oregon 97331mentioning

confidence: 93%

Reduced Glucose Transport in Sperm from Roosters (Gallus domesticus) with Heritable Subfertility1

McLean

Jones

Froman

1997

Biology of Reproduction

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Roosters homozygous for the rose comb allele (R/R) are subfertile. In previous research, these subfertile roosters were characterized by an in vitro sperm penetration assay as having limited sperm motility. The objectives in the present study were to characterize sperm motility by computer-assisted sperm motion analysis and to account for a mechanism underlying poor sperm motility. Percentages of motile sperm differed between subfertile males and fertile controls (r/r) by 29% (p < 0.001). The concentration of intracellular ATP in sperm form subfertile roosters was less than in that from fertile controls (p < 0.001). The genotypic difference is sperm motility, as measured with the sperm penetration assay, was maintained when ATP production was dependent on anaerobic glycolysis (p < 0.001). In this case, sperm were incubated with exogenous glucose and cyanide. Consequently, we could not attribute the genotypic difference in sperm mobility to mitochondrial respiration. In contrast, glucose transport, as measured by the uptake of [1,2-3H]-2-deoxy-D-glucose, was reduced in sperm from subfertile roosters (p < 0.001). Neither hexokinase nor glyceraldehyde-3-phosphate dehydrogenase activity differed between genotypes (p > 0.05). Likewise, lactate dehydrogenase activity did not differ between genotypes (p > 0.05). As evidenced by creatine kinase activity and dynein ATPase activity, neither the potential for energy transfer nor utilization within the axoneme differed between genotypes (p > 0.05). Therefore, we attribute the subfertility of roosters homozygous for the rose comb allele to decreased spermatozoal glucose transport.

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Comparative study of some characteristics of the semen of RR (rose comb) or rr (single comb) cockerels

Abstract: Introduction.

Cited by 9 publications

References 7 publications

Analysis of Subfertility Associated with Homozygosity of the Rose Comb Allele in the Male Domestic Fowl

Analysis of Subfertility Associated with Homozygosity of the Rose Comb Allele in the Male Domestic Fowl

Comparative metabolism of spermatozoa from subfertile Delaware and Wyandotte roosters

Reduced Glucose Transport in Sperm from Roosters (Gallus domesticus) with Heritable Subfertility1

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