Comparative study of serology and histology based detection of Helicobacter pylori infections: a large population-based study of 7,241 subjects from China

Gong, Yuehua; Sun, Liping; Jin, Shengfeng; Yuan, Yihao

doi:10.1007/s10096-010-0944-9

Cited by 30 publications

(22 citation statements)

References 9 publications

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“…Histological assay of H. pylori density was reported previously [25]. Briefly, H. pylori density was measured by H&E stain and was scored as follows: 0 point for no H. pylori infection, 1 point for H. pylori amounting to 5-10 bacteria in any gastric pit, 2 points for 10-100 and 3 points for more than 100.…”

Section: Endoscopic and Histopathological Examinationsmentioning

confidence: 99%

Serum anti-Helicobacter pyloriimmunoglobulin G titer correlates with grade of histological gastritis, mucosal bacterial density, and levels of serum biomarkers

Sun

Dong

et al. 2013

Scandinavian Journal of Gastroenterology

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OBJECTIVE. Clinical implications of serum anti-Helicobacter pylori immunoglobulin G (IgG) titer were unclear. This study investigated the associations of serum anti-H. pylori IgG titer with grade of histological gastritis, mucosal bacterial density and levels of serum biomarkers, including pepsinogen (PG) I, PGII, PGI/II ratio and gastrin-17. MATERIAL AND METHODS. Study participants were from a screening program in northern China. Serum anti-H. pylori IgG measurements were available for 5922 patients with superficial gastritis. Serum anti-H. pylori IgG titer and serum biomarkers were measured using ELISA, and gastric biopsies were evaluated using standardized criteria. RESULTS. In patients with mild, moderate or severe superficial gastritis, the mean serum anti-H. pylori IgG titers were 17.3, 33.4 and 54.4 EIU (p for trend < 0.0001), respectively. As mucosal H. pylori density score increased from 0 to 3, the mean serum anti-H. pylori IgG titers also increased from 24.7 to 44.8 EIU (p for trend < 0.0001). Serum anti-H. pylori IgG titer was associated positively with serum PGI, PGII and gastrin-17 concentrations and negatively with PGI/II ratio, and the association was the strongest for PGII. The mean PGII concentration of the patients in the highest quartile of IgG titer was twice the mean concentration of the patients in the lowest quartile (17.2 vs. 8.6 EIU, p < 0.0001). CONCLUSIONS. Our results suggest that serum anti-H. pylori IgG titer was associated positively with grade of histological gastritis, mucosal bacterial density and concentrations of serum PGI, PGII and gastrin-17, and negatively with PGI/II ratio.

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Section: Endoscopic and Histopathological Examinationsmentioning

confidence: 99%

Serum anti-Helicobacter pyloriimmunoglobulin G titer correlates with grade of histological gastritis, mucosal bacterial density, and levels of serum biomarkers

Sun

Dong

et al. 2013

Scandinavian Journal of Gastroenterology

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“…One possible explanation is the type of antigen used in the test kits was not similar. Serological kits developed from H. pylori strains from one region may not perform well in patients in other region due to difference in strain specific antigenecity [36][37][38][39][40]. Moreover, host immune response, duration of exposure, nutritional status, cross antigenicity with other prevalent antigenically related bacteria e.g.…”

Section: Discussionmentioning

confidence: 99%

Validation of diagnostic tests and epidemiology of Helicobacter pylori infection in Bangladesh

Aftab

Yamaoka

Ahmed

et al. 2018

J Infect Dev Ctries

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Introduction: Helicobacter pylori infection is associated with gastritis, peptic ulcer, and gastric cancer. We conducted a cross-sectional study to compare five diagnostic tests for H. pylori infection and studied the epidemiology of the infection in Bangladesh. Methodology: Bangladeshi patients with dyspeptic symptoms referred for endoscopic examination were enrolled in this study. Each patient underwent upper endoscopic examination and four gastric biopsy specimens were taken. We used 5 tests for the diagnosis of H. pylori; culture, histology confirmed by immunohistochemistry, rapid urease test (RUT), urinary and serological test. Demographic and environmental variables were collected. Results: A total of 133 patients participated in the study, 61 males and mean age 37.3 ± 12.3 years. We used the culture and/or histology results as the gold standard to estimate the sensitivity, specificity, positive and negative predictive values for the studied diagnostic tests. RUT, culture and histology had high sensitivity and specificity with moderate positive and negative likelihood ratio, whereas urine test and serology showed a good sensitivity and specificity but poor likelihood ratio. The overall prevalence of H. pylori among study subjects was 47% with no difference between gender and age groups. Conclusions: The invasive tests showed better performance than noninvasive tests among Bangladeshi population. The overall prevalence of H. pylori was less than the previously reported in the region with no difference among all age groups.

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“…The test of H pylori serology was performed to check the status of H pylori infection using enzyme-linked immunosorbent assay (ELISA, H pylori-IgG ELISA kit, BIOHIT Plc, Helsinki, Finland), as described previously (Gong et al, 2010). Briefly, approximately 5 ml fasting venous blood was obtained from each individual and the serum sample was collected after 10 min centrifugation at a speed of 3500×g.…”

Section: H Pylori Serology Examinationmentioning

confidence: 99%

The DNA Repair Gene ERCC6 rs1917799 Polymorphism is Associated with Gastric Cancer Risk in Chinese

Liu

He²,

Sun³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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Objective: Excision repair cross-complementing group 6 (ERCC6) is a major component of the nucleotide excision repair pathway that plays an important role in maintaining genomic stability and integrity. Several recent studies suggested a link of ERCC6 polymorphisms with susceptibility to various cancers. However, the relation of ERCC6 polymorphism with gastric cancer (GC) risk remains elusive. In this sex-and age-matched case-control study including 402 GC cases and 804 cancer-free controls, we aimed to investigate the association between a potentially functional polymorphism (rs1917799 T>G) in the ERCC6 regulatory region and GC risk. Methods: The genotypes of rs1917799 were determined by Sequenom MassARRAY platform and the status of Helicobacter pylori infection was detected by enzyme-linked immunosorbent assay. Odd ratios (ORs) and 95% confidential interval (CI) were calculated by logistic regression analysis. Results: Compared with the common TT genotype, the ERCC6 rs1917799 GG genotype was associated with increased GC risk (adjusted OR=1.46, 95%CI: 1.03-2.08, P=0.035). When compared with (GT+TT) genotypes, the GG genotype also demonstrated a statistical association with increased GC risk (adjusted OR=1.38, 95%CI: 1.01-1.89, P=0.044). This was also observed for the male subpopulation (GG vs. TT: adjusted OR=1.71, 95%CI: 1.12-2.62, P=0.013; G allele vs. T allele: adjusted OR=1.32, 95%CI: 1.07-1.62, P=0.009). Genetic effects on increased GC risk tended to be enhanced by H. pylori infection, smoking and drinking, but their interaction effects on GC risk did not reach statistical significance. Conclusions: ERCC6 rs1917799 GG genotype might be associated with increased GC risk in Chinese, especially in males.

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Comparative study of serology and histology based detection of Helicobacter pylori infections: a large population-based study of 7,241 subjects from China

Cited by 30 publications

References 9 publications

Serum anti-Helicobacter pyloriimmunoglobulin G titer correlates with grade of histological gastritis, mucosal bacterial density, and levels of serum biomarkers

Serum anti-Helicobacter pyloriimmunoglobulin G titer correlates with grade of histological gastritis, mucosal bacterial density, and levels of serum biomarkers

Validation of diagnostic tests and epidemiology of Helicobacter pylori infection in Bangladesh

The DNA Repair Gene ERCC6 rs1917799 Polymorphism is Associated with Gastric Cancer Risk in Chinese

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