Comparative Study of Puerariae lobatae and Puerariae thomsonii by HPLC-Diode Array Detection-Flow Injection-Chemiluminescence Coupled with HPLC-Electrospray Ionization-MS

Zhang, Cuiling; Ding, Xuehua; Hu, Ziyi; Wang, Xintang; Chen, Linlin; Qi, Jin; Yu, Boyang

doi:10.1248/cpb.59.541

Cited by 14 publications

(5 citation statements)

References 26 publications

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“…2 shows the chromatogram of the PR sample analyzed by SM-MCB, with significant area enhancement of 11 peaks (P1–P11) compared with the control containing denatured mitochondria (Δ P > 20%, shown in Table 1 ), suggesting specific binding with mitochondria. The chemical structure corresponding to each peak was assigned by analyzing UV, MS and MS n information obtained from LC/MS ( Table 2 ) and comparing it with previously reported data [28] , [29] , [30] , [31] , [32] and standards. Active compounds were identified as nine isoflavones ( P1 – P6 , P8 – P11 ) and one phenylpropanoid ( P7 ).…”

Section: Resultsmentioning

confidence: 99%

Screening potential mitochondria-targeting compounds from traditional Chinese medicines using a mitochondria-based centrifugal ultrafiltration/liquid chromatography/mass spectrometry method

Yang

Zhou

et al. 2018

Journal of Pharmaceutical Analysis

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Mitochondria regulate numerous crucial cell processes, including energy production, apoptotic cell death, oxidative stress, calcium homeostasis and lipid metabolism. Here, we applied an efficient mitochondria-based centrifugal ultrafiltration/liquid chromatography/mass spectrometry (LC/MS) method, also known as screening method for mitochondria-targeted bioactive constituents (SM-MBC). This method allowed searching natural mitochondria-targeting compounds from traditional Chinese medicines (TCMs), including Puerariae Radix (PR) and Chuanxiong Radix (CR). A total of 23 active compounds were successfully discovered from the two TCMs extracts. Among these 23 hit compounds, 17 were identified by LC/MS, 12 of which were novel mitochondria-targeting compounds. Among these, 6 active compounds were analyzed in vitro for pharmacological tests and found able to affect mitochondrial functions. We also investigated the effects of the hit compounds on HepG2 cell proliferation and on loss of cardiomyocyte viability induced by hypoxia/reoxygenation injury. The results obtained are useful for in-depth understanding of mechanisms underlying TCMs therapeutic effects at mitochondria level and for developing novel potential drugs using TCMs as lead compounds. Finally, we showed that SM-MBC was an efficient protocol for the rapid screening of mitochondria-targeting constituents from complex samples such as PR and CR extracts.

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Section: Resultsmentioning

confidence: 99%

Screening potential mitochondria-targeting compounds from traditional Chinese medicines using a mitochondria-based centrifugal ultrafiltration/liquid chromatography/mass spectrometry method

Yang

Zhou

et al. 2018

Journal of Pharmaceutical Analysis

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“…The glucoside conjugates of isoflavonoids, including O ‐glucosides and C ‐glucosides, and these are considered the major active components of Gegen []. To date, a large number of isoflavonoids in Gegen have been isolated and characterized, such as puerarin, 3′‐hydroxypuerarin, 6′′‐ O ‐xylosylpuerarin, mirificin, 3′‐methoxypuerarin, daidzin, daidzein, genistin, and genistein []. Puerarin, the first major isoflavonoid in Gegen, has been prescribed in China for the treatment of patients with cardiovascular diseases, cerebrovascular diseases, and hyperlipidaemia [] in the form of a puerarin injection.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of six isoflavonoids in rat plasma after administration of total flavonoid from Gegen by ultra‐HPLC‐MS/MS

Zhao¹,

Zhao²,

Liu³

et al. 2012

J of Separation Science

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A simple, specific, and sensitive ultra-performance liquid chromatography-tandem mass spectrometry method was developed for the simultaneous determination of 3'-hydroxypuerarin, 6''-O-xylosylpuerarin, mirificin, puerarin, 3'-methoxypuerarin and daidzin in rat plasma. After the addition of methanol containing 0.1% formic acid and 10% ascorbic acid, the analytes and rutoside were obtained by protein precipitation, then separated on a Thermo Syncronis C18 column (2.1 mm × 10 cm, 1.7 μm) by gradient elution and monitored using an electrospray ionization interface operating in positive ion and selective reaction monitoring acquisition mode. The calibration curves of these analytes showed good linearity (r > 0.99) within the test ranges. The lower limit of quantification was 0.0200 μg/mL for 3'-hydroxypuerarin, 0.0101 μg/mL for 6''-O-xylosylpuerarin, 0.0100 μg/mL for mirificin and puerarin, 0.0098 μg/mL for 3'-methoxypuerarin, and 0.0090 μg/mL for daidzin. The intraday and interday precision and accuracy were all within 15%. The extraction recoveries were from 74.0 to 95.8%. The validated method was successfully applied to pharmacokinetic studies of the six isoflavonoids in rat plasma after intravenous administration of total flavonoids from Gegen.

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“…Those 12 compounds were puerarin, puerarin-3 -methoxy-4 -O-glucoside, puerarin-4 -O-glucoside, 3 -methoxypuerarin, daidzein, daidzein-4 ,7-O-glucoside, daidzin, genistein, genistin, mirificin, sophoraside A, and ononin [18]. In addition, a study indicated that daidzein, mirificin, puerarin, 6 -O-xylosylpuerarin, 3 -hydroxypuerarin, genistin, and 8-C-glycoside-xyloside can be used as chemical markers for discrimination between P. lobata and P. thomsonii [19]. Furthermore, Zhang and his team reported seven isoflavones from roots of this plant using high-performance liquid chromatography (HPLC) analysis coupled with electrospray ionization ( ESI)-ion trap mass spectrometry analysis.…”

Section: Flavonoid Phytochemicalsmentioning

confidence: 99%

“…Moreover, in the classic medical book A Treatise on Fevers (Shang Han Lun), dated in 200 AD, the usage of Gegen Tang (a decoction containing kudzu root as the major component for curing neck stiffness, lack of perspiration, and aversion to air drafts) was mentioned [24]. It was employed to treat an alcohol-related problem, for example, as an anti-intoxication agent in 600 AD and an anti-dipsotropic agent in 1200 AD, as recommended by Li Shi-Zhen, the notorious physician, herbologist and acupuncturist in Chinese history [19,23]. Almost every part of kudzu has been used for many purposes.…”

Section: Traditional Uses (Past To Present)mentioning

confidence: 99%

A Promising View of Kudzu Plant, Pueraria montana var. lobata (Willd.) Sanjappa & Pradeep: Flavonoid Phytochemical Compounds, Taxonomic Data, Traditional Uses and Potential Biological Activities for Future Cosmetic Application

Intharuksa

Sasaki

2020

Cosmetics

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Pueraria montana var. lobata is widely known as kudzu especially in Japan, China, Korea, and other Asian countries. This plant is an ingredient for traditional food and an herbal ingredient for traditional medicines, particular in Japan and China. There are a few reports on its cosmetic uses. Interestingly, many phytochemical compounds from this plant have been continuously reported, particularly flavonoid compounds, which are well-known as potential bioactive ingredients for cosmetics. This work aims to illustrate promising views of kudzu plant, focusing on the diversity of flavonoid phytochemical compounds, taxonomic data, traditional uses, and potential biological activities for future cosmetic applications, i.e., antioxidant, antiglycation, skin regeneration, and melanogenesis inhibitory activities.

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Comparative Study of Puerariae lobatae and Puerariae thomsonii by HPLC-Diode Array Detection-Flow Injection-Chemiluminescence Coupled with HPLC-Electrospray Ionization-MS

Cited by 14 publications

References 26 publications

Screening potential mitochondria-targeting compounds from traditional Chinese medicines using a mitochondria-based centrifugal ultrafiltration/liquid chromatography/mass spectrometry method

Screening potential mitochondria-targeting compounds from traditional Chinese medicines using a mitochondria-based centrifugal ultrafiltration/liquid chromatography/mass spectrometry method

Simultaneous determination of six isoflavonoids in rat plasma after administration of total flavonoid from Gegen by ultra‐HPLC‐MS/MS

A Promising View of Kudzu Plant, Pueraria montana var. lobata (Willd.) Sanjappa & Pradeep: Flavonoid Phytochemical Compounds, Taxonomic Data, Traditional Uses and Potential Biological Activities for Future Cosmetic Application

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