Comparative study of <i>Microsporum canis</i> isolates by <scp>DNA</scp> fingerprinting

Shafiee, Shabnam; Khosravi, Alireza; Tamai, Iradj Ashrafi

doi:10.1111/myc.12189

Cited by 11 publications

(8 citation statements)

References 18 publications

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“…Microsporium canis is a pathogenic dermatophyte that infects the upper dead layers of the skin of domesticated cats and occasionally dogs, horses and humans [24,25]. It has been identified as a causal agent of ringworm infections in pets, tinea capitis and tinea corporis in humans particularly in children.…”

Section: Discussionmentioning

confidence: 99%

Diversity of Fungi in the Public Hand-pump Borehole Water in Onueke, Ezza Local Government Area, Ebonyi State, Nigeria

Samuel¹,

John²,

Frederick³

2019

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The diversity of fungi in fifteen public hand-pump borehole water in Onueke, Ezza Local Government Area of Ebonyi State, Nigeria was studied during the dry and wet seasons using standard analytical procedures. The total moulds and yeasts counts were 2-8cfu/ml and 1-5cfu/ml respectively during the dry season and 3-12cfu/ml and 1-4cfu/ml respectively during the wet season. More moulds were isolated from the samples during the wet than the dry season while more yeasts were isolated during the dry than the wet season. The fungi were characterized and identified on the basis of their colonial, microscopic, biochemical and molecular characteristics as Candida albicans, Microsporum canis, Aspergillus fumigatus, Geotrichum candidum, Basidiobolus ranarum, Microsporum audouinii, Fonsecaea pedrosoi and Trichosporon cutaneum. More of the boreholes contained Candida albicans than the other isolates during both seasons. Candida albicans occurred most frequently in the water samples during both seasons while Fonsecaea pedrosoi had the lowest frequency of occurrence in the water samples during both seasons. All the isolates were resistant to Griseofulvin while they exhibited varying degree of resistance and sensitivity to the other antifungal agents used. This study indicated that the borehole water samples examined did not comply with the standard established by regulatory bodies for potable water and therefore must be adequately treated before drinking and use for domestic purposes as these fungi have been reported to cause diseases of humans and animals. Boiling, chlorination, sand filtration and exposure to ultraviolet radiation are recommended.

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Section: Discussionmentioning

confidence: 99%

Diversity of Fungi in the Public Hand-pump Borehole Water in Onueke, Ezza Local Government Area, Ebonyi State, Nigeria

Samuel¹,

John²,

Frederick³

2019

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“…These methods can also be used in many cases for species identification of dermatophytes and clarification of the relationships among varieties of a species . The application of PCR‐fingerprinting to dermatophytes should be particularly useful because members of this group of fungal pathogens are closely related and often extremely difficult to identify on the basis of morphological differences . In addition, one of the diagnostic elements in the case of dermatophytosis is to determine the source of infection in order to eliminate it .…”

Section: Discussionmentioning

confidence: 99%

Tinea corporis by Microsporum canis in mycological laboratory staff: Unexpected results of epidemiological investigation

Gnat

Łagowski

Nowakiewicz

et al. 2018

Mycoses

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Microsporum canis is a zoophilic dermatophyte, which is very contagious, especially to cats and dogs. Asymptomatic animal carriers of M. canis are regarded a critical factor in the epidemiology of the disease. The aim of this study was to investigate the epidemiological origin of M. canis isolates using morphological traits in combination with molecular analysis. Identification of dermatophyte strains was carried out by correlating the clinical manifestation of the infection with a micro- and macroscopic examination. To confirm the species affiliation fully, molecular differentiation methods were used. A positive result of the culture examination was obtained from the samples with arthrospores in the direct analysis, that is, from a symptomatic cat and humans, and from a cat without any signs of infection. The microsatellite-primed PCR fingerprinting (MSP-PCR) electro-profiles were identical for all analysed strains. The melting profile-PCR (MP-PCR) electrophoregram indicated variability of the genomes of the strains. The search for the source of the infection indicated one cat that did not have any signs of dermatophytosis. PCR-fingerprinting techniques are useful tools for epidemiological investigation of the origin of dermatophyte infection. These methods can also be used in many cases for species identification of dermatophytes and clarification of the relationships among varieties of a species.

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“…Microsporium canis is a pathogenic dermatophyte that infects the upper dead layers of the skin of domesticated cats and occasionally dogs and humans [17]. It has been identified as a casual agent of ringworm infections in pets, tinea capitis and tinea corporis in humans particularly in children [18].…”

Section: Discussionmentioning

confidence: 99%

Mycology of Hand-dug Shallow Water Wells in Awka Metropolis, Anambra State, Nigeria

Samuel¹,

Rosemary²,

Frederick³

2016

azb

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Samples collected from hand-dug shallow water wells in Awka metropolis during the dry and wet seasons were subjected to mycological analysis to determine their safety for use. The fungal counts during the dry and wet seasons were 3.5x10 3 cfu/ml-8.2x10 3 cfu/ml and 5.1x10 3 cfu/ml-10.6x10 3 cfu/ml respectively. The fungi isolated during the dry and wet seasons respectively were Candida albicans (42.0% and 44.8%), Cryptococcus neoformans (9.6% and 10.2%), Rhodotorula Harrison (11.5% and 12.6%), Microsporium canis (0.0% and 0.2%), Penicillium chrysogenum (28.0% and 29.3%), Alternaria alternata (0.0% and 0.3%), Mucor mucedo (8.9% and 9.2%) and Sepedonium chrysospermum (0.0% and 0.2%). Candida albicans, Cryptococcus neoformans, Rhodotorula harrison, Penicillium chrysogenum and Mucor mucedo were isolated from 46.7%, 6.7%, 20.0%, 26.7% and 6.7% of the samples respectively during the dry season while 60.0%, 20.0%, 26.7%, 6.7%, 40.0%, 13.3%, 20.0% and 6.7% of the samples respectively had Candida albicans, Cryptococcus neoformans, Rhodotorula harrison, Microsporium canis, Penicillium chrysogenum, Alternaria alternata, Mucor mucedo and Sepedonium chrysospermum. These fungi are human pathogens which are known to cause diseases of animals and humans which if untreated may lead to fatal consequences, therefore adequate treatment of the water will safeguard the health of the users.

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Comparative study of Microsporum canis isolates by DNA fingerprinting

Cited by 11 publications

References 18 publications

Diversity of Fungi in the Public Hand-pump Borehole Water in Onueke, Ezza Local Government Area, Ebonyi State, Nigeria

Diversity of Fungi in the Public Hand-pump Borehole Water in Onueke, Ezza Local Government Area, Ebonyi State, Nigeria

Tinea corporis by Microsporum canis in mycological laboratory staff: Unexpected results of epidemiological investigation

Mycology of Hand-dug Shallow Water Wells in Awka Metropolis, Anambra State, Nigeria

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