Comparative study of human red blood cell analysis with three different field-flow fractionation systems

Parsons, Robert G.; Yue, Vincent; Tong, Xiaomi; Cardot, Philippe; Bernard, Agnes; Andreux, J.P.; Caldwell, Karin D.

doi:10.1016/s0378-4347(96)00186-7

Cited by 21 publications

(8 citation statements)

References 11 publications

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“…On the other hand, the empirical formula for HD lift forces derived by Williams et al (1992) predicts that PS beads should have been levitated to much higher positions than we observed. Furthermore, our findings that the HD lift force is independent of fluid flow rate and increases with decreasing fluid viscosity are contrary to the experimental results in several other reports (Caldwell et al, 1979;Parsons et al, 1996;Tong and Caldwell, 1995;Williams et al, 1992). The physical nature of the HD lift effect and the reasons for the discrepancies between our experimental data and these earlier findings are not understood.…”

Section: Hydrodynamic Lift Forcescontrasting

confidence: 99%

“…Field-flow-fractionation has been used for separation and characterization of a number of types of biological cells including Escherichia coli, human and animal erythrocytes, cultured human HeLa, and Chinese hamster ovary CHO-K1 cells (Andreux et al, 1993;Berg and Turner, 1991;Caldwell et al, 1984;Cardot et al, 1992;Parsons et al, 1996;Tong et al, 1997). FFF studies of human erythrocytes have revealed that cell membrane deformability is an important factor contributing to the hydrodynamic lifting force acting on the cells as they travel in the flow stream at positions close to the channel walls (Tong and Caldwell, 1995).…”

Section: Introductionmentioning

confidence: 99%

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Separation of Polystyrene Microbeads Using Dielectrophoretic/Gravitational Field-Flow-Fractionation

Wang

Vykoukal

Becker

et al. 1998

Biophysical Journal

161

164

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The characterization of a dielectrophoretic/gravitational field-flow-fractionation (DEP/G-FFF) system using model polystyrene (PS) microbeads is presented. Separations of PS beads of different surface functionalization (COOH and none) and different sizes (6, 10, and 15 microm in diameter) are demonstrated. To investigate the factors influencing separation performance, particle elution times were determined as a function of particle suspension conductivity, fluid flow rate, and applied field frequency and voltage. Experimental data were analyzed using a previously reported theoretical model and good agreement between theory and experiment was found. It was shown that separation of PS beads was based on the differences in their effective dielectric properties. Particles possessing different dielectric properties were positioned at different heights in a fluid-flow profile in a thin chamber by the balance of DEP and gravitational forces, transported at different velocities under the influence of the fluid flow, and thereby separated. To explore hydrodynamic (HD) lift effects, velocities of PS beads were determined as a function of fluid flow rate in the separation chamber when no DEP field was applied. In this case, particle equilibrium height positions were governed solely by the balance of HD lift and gravitational forces. It was concluded that under the experimental conditions reported here, the DEP force was the dominant factor in controlling particle equilibrium height and that HD lift force played little role in DEP/G-FFF operation. Finally, the influence of various experimental parameters on separation performance was discussed for the optimization of DEP/G-FFF.

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Section: Hydrodynamic Lift Forcescontrasting

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Separation of Polystyrene Microbeads Using Dielectrophoretic/Gravitational Field-Flow-Fractionation

Wang

Vykoukal

Becker

et al. 1998

Biophysical Journal

161

164

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“…In our literature search, we have found an association between elevated RDW and altered hemorheological features (for example, increased total blood viscosity [17], red blood cell agglutination and fragmentation [12,18]). In addition, elevated RDW was found to be associated with increased retention ratios in field flow-fractionation systems [19]. Moreover, high RDW was associated with platelet anisocytosis [20].…”

Section: Discussionmentioning

confidence: 99%

Usefulness of Red Cell Distribution Width in Predicting All-Cause Long-Term Mortality after Non-ST-Elevation Myocardial Infarction

Azab¹,

Torbey²,

Hatoum³

et al. 2011

Cardiology

100

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Background: Red blood cell distribution width (RDW) is a strong predictor of adverse outcomes in patients with heart failure, stable coronary artery disease, stroke and acute myocardial infarction. The aim of our study was to explore the predictive value of RDW on all-cause mortality in patients with non-ST-segment elevation myocardial infarction (NSTEMI). Method: This observational study includes 619 NSTEMI patients, discharged from Staten Island University Hospital between September 2004 and December 2006. Patients were divided into equal RDW tertiles and survival was evaluated in each tertile. Result: Patients in the highest RDW tertile (RDW >14) had higher in-patient (7 vs. 1%) and 4-year (30 vs. 7%) mortality rates compared to those in the lowest tertile (RDW <13) (Wilcoxon χ² = 34.64, p < 0.0001). After controlling for Global Registry of Acute Coronary Events risk profile scores and other confounding variables, the RDW adjusted hazard ratio for 4-year all-cause mortality increased by 1.10 for each one unit increase in RDW (confidence interval 1.004–1.213, p = 0.042). Conclusion: RDW is an independent predictor of all-cause long-term mortality in NSTEMI patients. Further studies are needed to clarify the mechanisms of this association between RDW and adverse outcomes in patients with coronary artery disease.

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“…Unlike gravitational and sedimentation FFF (Yue et al, 1994;Parsons et al, 1996), in which separation performance depends sensitively on cell density, DEP-FFF used cell density through sedimentation forces to act in balance with DEP levitation forces, and cell density is not the only factor that determines separation performance. Take granulocytes and monocytes as an example.…”

Section: Cell Separation Mechanicsmentioning

confidence: 99%

Differential Analysis of Human Leukocytes by Dielectrophoretic Field-Flow-Fractionation

Yang

Huang²,

Wang³

et al. 2000

Biophysical Journal

204

162

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The differential analysis of human leukocytes has many important biological and medical applications. In this work, dielectrophoretic field-flow-fractionation (DEP-FFF), a cell-separation technique that exploits the differences in the density and dielectric properties of cells, was used to separate the mixtures of the major human leukocyte subpopulations (T- and B-lymphocytes, monocytes, and granulocytes). The separation was conducted in a thin chamber equipped with an array of microfabricated interdigitated electrodes on the bottom surface, and the separation performance was characterized by on-line flow cytometry. To investigate optimal separation conditions for different leukocyte mixtures, elution fractograms at various DEP field frequencies were obtained for each leukocyte subtype. With appropriately chosen conditions, high separation performance was achieved in separating T- (or B-) lymphocytes from monocytes, T- (or B-) lymphocytes from granulocytes, and monocytes from granulocytes. DEP-FFF does not involve cell-labeling or cell-modification step, and provides a new approach to hematological analysis.

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Comparative study of human red blood cell analysis with three different field-flow fractionation systems

Cited by 21 publications

References 11 publications

Separation of Polystyrene Microbeads Using Dielectrophoretic/Gravitational Field-Flow-Fractionation

Separation of Polystyrene Microbeads Using Dielectrophoretic/Gravitational Field-Flow-Fractionation

Usefulness of Red Cell Distribution Width in Predicting All-Cause Long-Term Mortality after Non-ST-Elevation Myocardial Infarction

Differential Analysis of Human Leukocytes by Dielectrophoretic Field-Flow-Fractionation

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