Comparative study of hematological parameters of Colossoma macropomum anesthetized with benzocaine and eugenol by using different anticoagulants

Sousa, Andria Gama; Pacheco, Aline; Siqueira-Pinto, Gilson A.; Reis, Gleika Tamires Jordão; Fugimura, Michelle Midori Sena; Jensen, Luciano; Marcusso, Paulo Fernandes; Ramos‐Espinoza, Fernando Carlos; Claudiano, Gustavo da Silva

doi:10.1007/s10499-021-00668-8

Cited by 2 publications

(4 citation statements)

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“…One aliquot (1.0 ml −1 /sample) destined for blood counts and reactive oxygen species (ROS) quantification was stored in a tube containing ethylene-diamino-tetraacetic acid (EDTA k3). The other portion (2.0 ml −1 /sample) was placed in a dry tube and centrifuged at 1.200 g, for 5 min, at 4ºC, to obtain serum, which was then stored at -80ºC (Sousa et al, 2021). After the blood collection (n = 10/group), the fish were euthanized by an anaesthetic overdose and six fish per treatment underwent aseptic necropsies in order to collect their intestines (Marinho-Neto et al, 2019).…”

Section: Fish Probiotic Supplementation and Experimental Designsmentioning

confidence: 99%

“…Total red blood cell (RBC, 10 6 cells/µL) counts were performed in a Neubauer chamber using Natt and Herrik's diluent (1:200) (Sousa et al, 2021). Aliquots were used for haematocrit (Htc %) (Goldenfarb et al, 1971) and haemoglobin concentration (Drabkin, 1935) determinations.…”

Section: Blood and Serum Biochemical Assessmentsmentioning

confidence: 99%

“…Additionally, blood smears were stained by rapid panoptic staining for total thrombocyte, total leukocyte and differential leukocyte (neutrophils, monocytes and lymphocytes) counts by light microscopy, through an indirect method (Sousa et al, 2021). In this method, 2,000 erythrocytes were counted in blood extensions for total leukocyte and thrombocyte determinations as follows: Total leukocytes (μL) = No.…”

Section: Blood and Serum Biochemical Assessmentsmentioning

confidence: 99%

“…de thrombocytes in extension × No. erythrocytes count in the Neubauer chamber (μL) / 2,000 erythrocytes in extension (Sousa et al, 2021).…”

Section: Blood and Serum Biochemical Assessmentsmentioning

confidence: 99%

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Effect of multi‐species probiotic administration in Colossoma macropomum juvenile rearing: supplementation and bioremediation

et al. 2021

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Probiotics are defined as live microbial supplements that benefit the host (Fuller, 1989). In aquaculture, the definition is more comprehensive due to the fact that probiotics are applied by different means, such as feeding, injection or immersion in the culture water. Research on the use of probiotics in animal husbandry has increased with the increasing demands for environmentally sound agriculture/aquaculture practices and safer food (Liu & Han, 2004), especially when substituting antibiotics and growth promoters (Akhter et al., 2015).Fish gastrointestinal tract probiotics play an important role in improving zootechnical performance, ingredient digestibility, disease resistance and immune system responses (Mello et al., 2013;Silva et al., 2015). Furthermore, direct probiotic addition to fish farming water can result in several effects, with two main advantages, namely the ability to control water quality by bioremediation and pathogen biocontrol through antagonistic effects (Jahangiri & Esteban, 2018;Liu & Han, 2004).Studies on aquaculture-applied probiotics are of great interest in the aquaculture industry and market, and several options are

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Section: Fish Probiotic Supplementation and Experimental Designsmentioning

confidence: 99%

Section: Blood and Serum Biochemical Assessmentsmentioning

confidence: 99%

Section: Blood and Serum Biochemical Assessmentsmentioning

confidence: 99%

“…de thrombocytes in extension × No. erythrocytes count in the Neubauer chamber (μL) / 2,000 erythrocytes in extension (Sousa et al, 2021).…”

Section: Blood and Serum Biochemical Assessmentsmentioning

confidence: 99%

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