Comparative study of four HDL-cholesterol assay methods

P, Chicaud; Lambert, Daniel; Méjean, L.; Drouin, P; Debry, G

doi:10.1016/0009-8981(81)90168-6

Cited by 10 publications

(5 citation statements)

References 13 publications

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“…Serum apolipoprotein B containing lipoproteins were selectively precipitated by concanavalin A (3). After centrifugation, HDLPresented at ECCO-4, Madrid, November 1-4, 1987. cholesterol (HDL-C) was measured in the supernatant by an enzymatic method.…”

Section: Methodsmentioning

confidence: 99%

Proton Nuclear Magnetic Resonance Spectroscopy of Plasma Lipoproteins in Malignancy

Rossignol

Farnier

et al. 1988

Acta Oncologica

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Section: Methodsmentioning

confidence: 99%

Proton Nuclear Magnetic Resonance Spectroscopy of Plasma Lipoproteins in Malignancy

Rossignol

Farnier

et al. 1988

Acta Oncologica

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“…However, the density separa tion determines lipoproteins of different atherogenicity. Some differences between HDL chol and LpA chol have been pointed out [5]; this can be explained by inclusion in the frac tion of traces of Lp(a) (1.080 < d < 1.120) and other lipoproteins, considering the heter ogeneity of lipoprotein classes [1],…”

Section: Discussionmentioning

confidence: 99%

“…Lipoproteins containing Apo A are not precipitated. The method has been recently described [5]. Four parameters related to cholcstcrolemia can be calculated: LpA chol.…”

Section: Precipitation and Assay Methodsmentioning

confidence: 99%

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Application of the Concanavalin A Precipitation Technique in an Evaluation of Fenofibrate Action on Cholesterolemia in Ha and IIb Hyperlipemic Subjects

P¹,

Demange²,

Drouin³

et al. 1983

Pharmacology

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Total plasma cholesterol and lipoprotein fractions were studied before and after 4 months fenofibrate (Lipanthyl®) treatment of 34 hypercholesterolemic adults (16 IIa and 18 IIb), using a concanavalin A precipitation technique. The total plasma cholesterol levels decreased significantly for both IIa and IIb (24 and 18%, respectively, p < 0.001). Fenofibrate reduced the apoprotein B bound cholesterol in IIa and IIb (29 and 27%, respectively, p < 0.001). Apoprotein A bound cholesterol increased significantly in lib (29%, p < 0.001), but there was no significant increase in Ila (11 %). Triglycerides were significantly reduced in both groups. In the light of concanavalin A specificity for apoprotein B and the utility of this technique, it is suggested that the concanavalin A precipitation technique may be applied to hyperlipidemic subjects undergoing treatment with lipid-lowering agents.

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“…Serum total cholesterol was assayed by an enzy matic method and high density lipoprotein (HDL) cholesterol was assayed by the same method after selective precipitation of Apo B-containing lipopro teins by concanavalin A [9]. Scrum very low density lipoprotein (VLDL) + low density lipoprotein (LDL) cholesterol concentration was obtained by difference.…”

Section: Methodsmentioning

confidence: 99%

Serum Lipoproteins in Venous Blood Serum from Birth to the End of the First Week: Feeding Influences

Tenenbaum

Gambert²,

Meunier³

et al. 1988

Neonatology

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Serum lipoproteins were explored on the first day of life (D1) and on the sixth day (D6) in blood drawn by peripheral venipuncture from 43 normal term newborns, 22 breast-fed and 21 formula-fed, and were compared to those of a control group of 28 young adults. With the exception of apolipoprotein E (Apo E), values of lipoprotein components obtained at D1 were similar, although generally slightly lower than those previously reported for cord blood serum. Total Apo E concentration at D1 (71 ± 25 mg/l) was much higher than that obtained for the adult group (30 ± 7 mg/l). Apo E distribution within the lipoprotein spectrum confirmed the presence of an already known Apo E-rich high density lipoprotein subfraction, which was responsible for the high total Apo E level at birth. The rise of lipoproteins of low density from D1 to D6, as evidenced by the increase of very low density lipoprotein + low density lipoprotein cholesterol and low density lipoprotein apolipoprotein B concentrations, was shown to be diet dependent. It was significantly less important in newborns fed a standard formula moderately enriched in unsaturated fatty acids than in breast-fed newborns.

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Comparative study of four HDL-cholesterol assay methods

Cited by 10 publications

References 13 publications

Proton Nuclear Magnetic Resonance Spectroscopy of Plasma Lipoproteins in Malignancy

Proton Nuclear Magnetic Resonance Spectroscopy of Plasma Lipoproteins in Malignancy

Application of the Concanavalin A Precipitation Technique in an Evaluation of Fenofibrate Action on Cholesterolemia in Ha and IIb Hyperlipemic Subjects

Serum Lipoproteins in Venous Blood Serum from Birth to the End of the First Week: Feeding Influences

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