Comparative study of different alcoholate pretreatments for enhanced enzymatic hydrolysis of sugarcane bagasse

Huang, Qing; Yan, Qiuli; Fu, Jing; Lv, Xiaojing; Xiong, Chunjiang; Lin, Jianghai; Liu, Zehuan

doi:10.1016/j.biortech.2016.03.067

Cited by 20 publications

(6 citation statements)

References 33 publications

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“…The implication is that alkaline environment helped to increase the digestibility of sugarcane, possibly by reducing ADF and LIG, which corroborates findings of Magalhães et al (2013). Huang et al (2016) evaluated the effect of additives in the treatment of sugarcane bagasse (SCB) and reported that the C 2 H 5 ONa pre-treatment showed the best effect on hydrolysis of SCB. Under optimal conditions of 40 g kg À1 C 2 H 5 ONa, there was a reduction of fibrosis compounds.…”

Section: Discussionsupporting

confidence: 62%

Nutritional profiles of three genotypes of sugarcane silage associated with calcium oxide

et al. 2017

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The objective of this study was to evaluate, based on chemical composition and in vitro dry matter (DM) digestibility, silages of different sugarcane genotypes after exposure to different doses of calcium oxide (CaO) and to identify that most appropriate dose for use in ruminant feed. The experiment was conducted using a completely randomized 3 9 4 factorial arrangement with three sugarcane genotypes ('IAC-862480', 'SP-791011' and 'CTC-3') and four levels of the additive CaO (0, 15, 30 and 45 g kg À1 ). A quadratic effect was observed (P < 0.05) for DM content in the silages of genotypes 'SP-791011' and 'CTC-3'. The organic matter content of genotypes 'SP-791011' and 'CTC-3' showed a negative linear effect that was associated with the addition of CaO, which promoted an increase in the mineral matter (MM) content in the silages. There was no effect (P > 0.05) of genotype or an interaction effect between genotype and CaO level on crude protein content in the silages. There was an interaction effect (P < 0.05) between genotype and CaO level on the level of neutral detergent fiber in the studied silages and the development of this interaction revealed a linear decrease in neutral detergent fiber in genotype IAC-86280 and a quadratic effect (P < 0.05) for genotypes 'SP-791011' and 'CTC-3'. Potentially degradable DM (pdDM) values showed a positive quadratic function with maxima at 34.8 g kg À1 CaO. The results obtained for potentially degradable neutral detergent fiber (pdNDF) showed that there was a negative linear effect for genotype 'SP-791011' and a quadratic effect with the maximum critical point for genotypes 'IAC-862480' and 'CTC-3'. Total DM losses decreased with levels of CaO. All genotypes evaluated showed potential for silage production. The addition of CaO in the ensiling of sugarcane at up to 45 g kg À1 reduces gas and effluent losses and increases the DM recovery of the silages.

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Section: Discussionsupporting

confidence: 62%

Nutritional profiles of three genotypes of sugarcane silage associated with calcium oxide

et al. 2017

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“…4. Previous studies reported in the literature have shown that in the enzymatic hydrolysis process the highest glucose yield occurs in the first hours of saccharification, which is a period that β-glucosidase has maximum activity (Huang et al, 2016;Siqueira et al, 2013).…”

Section: β-Glucosidase Characterizationmentioning

confidence: 99%

“…However, these authors performed the enzymatic hydrolysis for a longer time (96 h) at 50°C and used enzymatic extracts produced by Penicillium oxalicum EU2106. In another study, Huang et al (2016) reported a concentration of TRS of 20.2 g L −1 after saccharification of sugarcane bagasse pretreated with sodium ethoxide (CH 3 CH 2 ONa), using a commercial enzyme cocktail (Celluclast 1.5 L and Novozym 188) under the hydrolysis conditions of 72 h, 45°C and 3% (w v −1 ) of pretreated bagasse. Comparing the TRS concentrations and yields obtained in the present study with those reported in the literature, it can be concluded that the enzymatic hydrolysis performed in the present study was efficient with an appreciable release of TRS in a relatively shorter time.…”

Section: Total Reducing Sugars Yield (Trs)mentioning

confidence: 99%

Synergistic action of an Aspergillus (hemi-)cellulolytic consortium on sugarcane bagasse saccharification

Rodrigues

Pereira

Santos

et al. 2017

Industrial Crops and Products

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This study evaluated the synergistic efficiency of crude enzymatic extracts (CEE) produced by fungal strains Aspergillus fumigatus SCBM6 (AFE) and Aspergillus niger SCBM1 (ANE) in the hydrolysis of alkali pretreated sugarcane bagasse (2% NaOH (w v −1), ∼100°C, 4 h and liquid-to-solid ratio of 25 (v w −1)). CEEs were produced by solid-state fermentation (SSF) and presented high concentration of β-glucosidase (AFE = 78.4 U g −1 and ANE = 43.02 U g −1), β-xylosidase (ANE = 78.0 U g −1) and xylanase (AFE = 1774.5 U g −1 and ANE = 3289.6 U g −1). A 2 4 full design of experiments was used to optimize the hydrolysis of pretreated bagasse as a function of time, temperature, amount of pretreated bagasse and AFE-to-ANE ratio. After hydrolysis optimization, the maximum concentration of total reducing sugars (TRS) was 21.68 g L −1. It was also noticed a significant production of xylose (135.59 mg) and arabinose (58.10 mg) per gram of hemicelluloses and a slight production of glucose (20.18 mg) per gram of cellulose. As fungi consortium were excellent producers of hemicellulases, it is suggested that they could be used for pentoses saccharification and production of ethanol via C-5 fermentation, xylitol, biopolymers, among other bioproducts.

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“…Similar improvement in the amount of liberated reducing sugars was observed by Rodrigues et al (2017) after an enzymatic hydrolysis carried out with increased SCB percentage, suggesting that high solid loading can improve saccharification yields. In all the enzymatic assays, the production of TRS was noteworthy in comparison to earlier studies Huang et al (2016) obtained 20.20 g/L of TRS after an enzymatic hydrolysis of 3% of sodium ethoxide pre-treated SCB using the commercial cocktails Celluclast 1.5 L and Novozymes 188 (1 FPU/4 ρNPGU) at 45°C, for 72 h. Silva et al (2017) achieved about 16.00 g/L of TRS after the hydrolysis in similar conditions to the present study (3% of SCB pre-treated with steam blast and NaOH, 10 FPU/g of cellulose of Cellic Ctec3, at 50 °C for 24 h). Interestingly, these authors also carried out enzymatic hydrolysis for 72 h at the same conditions and obtained 23.05 g/L of TRS, indicating that an increase in the hydrolysis time positively affected TRS release.…”

Section: Enzymatic Hydrolysismentioning

confidence: 44%

Effects of the Increase in Substrate Load and Hydrolysis Time in the Saccharification of Sugarcane Bagasse and Ethanol Production

Lamounier

Pasquini

Rodrigues

et al. 2020

HOLOS

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Comparative study of different alcoholate pretreatments for enhanced enzymatic hydrolysis of sugarcane bagasse

Cited by 20 publications

References 33 publications

Nutritional profiles of three genotypes of sugarcane silage associated with calcium oxide

Nutritional profiles of three genotypes of sugarcane silage associated with calcium oxide

Synergistic action of an Aspergillus (hemi-)cellulolytic consortium on sugarcane bagasse saccharification

Effects of the Increase in Substrate Load and Hydrolysis Time in the Saccharification of Sugarcane Bagasse and Ethanol Production

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