Comparative study of bovine, porcine and avian collagens for the production of a tissue engineered dermis

Parenteau-Bareil, Rémi; Gauvin, Robert; Cliché, Simon; Gariépy, C.; Germain, Lucie; Berthod, François

doi:10.1016/j.actbio.2011.06.020

Cited by 85 publications

(66 citation statements)

References 55 publications

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“…12,20,37 Relationships between collagen source and polymerization kinetics, hydrogel mechanics, and fiber structure are discussed in more detail in ''Polymerization,'' ''Mechanics,'' and ''Structure'' sections. Further, the method by which collagen is extracted from tissue has been shown to alter the molecular structure of the collagen fibrils as well as the kinetics of assembly.…”

Section: Collagen Source and Solubilizationmentioning

confidence: 99%

“…34,40,68 Further, manual estimation of dimensions of user-identified fibers and pores is not uncommon. 12,13,21,34,37,54,62,75 Finally, existing algorithms do not appear to be robust enough to accurately analyze images with a high density of fibers, a parameter primarily regulated by collagen concentration. For example, although Kreger et al investigated polymerization kinetics and mechanical properties of 0.5-4 mg/mL hydrogels, fiber structure data are notably absent for samples above 3 mg/mL.…”

Section: Figmentioning

confidence: 99%

See 1 more Smart Citation

Review of Collagen I Hydrogels for Bioengineered Tissue Microenvironments: Characterization of Mechanics, Structure, and Transport

Antoine

Vlachos

Rylander³

2014

Tissue Engineering Part B: Reviews

464

353

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Type I collagen hydrogels have been used successfully as three-dimensional substrates for cell culture and have shown promise as scaffolds for engineered tissues and tumors. A critical step in the development of collagen hydrogels as viable tissue mimics is quantitative characterization of hydrogel properties and their correlation with fabrication parameters, which enables hydrogels to be tuned to match specific tissues or fulfill engineering requirements. A significant body of work has been devoted to characterization of collagen I hydrogels; however, due to the breadth of materials and techniques used for characterization, published data are often disjoint and hence their utility to the community is reduced. This review aims to determine the parameter space covered by existing data and identify key gaps in the literature so that future characterization and use of collagen I hydrogels for research can be most efficiently conducted. This review is divided into three sections: (1) relevant fabrication parameters are introduced and several of the most popular methods of controlling and regulating them are described, (2) hydrogel properties most relevant for tissue engineering are presented and discussed along with their characterization techniques, (3) the state of collagen I hydrogel characterization is recapitulated and future directions are proposed. Ultimately, this review can serve as a resource for selection of fabrication parameters and material characterization methodologies in order to increase the usefulness of future collagenhydrogel-based characterization studies and tissue engineering experiments.

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Section: Collagen Source and Solubilizationmentioning

confidence: 99%

Section: Figmentioning

confidence: 99%

Review of Collagen I Hydrogels for Bioengineered Tissue Microenvironments: Characterization of Mechanics, Structure, and Transport

Antoine

Vlachos

Rylander³

2014

Tissue Engineering Part B: Reviews

464

353

View full text Add to dashboard Cite

show abstract

“…15 In human dermal microvascular endothelial cells and in a mouse model of skin angiogenesis, an interaction between endothelial cells and three-dimensional Col I has been shown to induce activation of MAP kinase pathways that promote angiogenesis. 16,17 Intact Col I has been shown to bind a number of proteases and inflammatory cytokines that are in excess in chronic wound fluid, including neutrophil elastase, MMP-2, interleukin (IL)-6, IL-8, and IL-1b as well as scavenging superoxide anion and peroxynitrate.…”

Section: Individual Matrix Componentsmentioning

confidence: 99%

Topical Collagen-Based Biomaterials for Chronic Wounds: Rationale and Clinical Application

Gould

2016

Advances in Wound Care

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“…Collagen 2 is a potentially useful biomaterial since it is a major constituent of the connective tissue. The main applications of collagen as drug delivery systems are collagen-shields in ophthalmology, sponges for burns/wounds and liposome collagen associations for transdermal and sustained drug delivery [4,5,6]. Usnic acid (UA), [2,6-…”

Section: Introductionmentioning

confidence: 99%

“…These studies generally involved the development and/or the physical characterization of the materials. Besides, some studies have been carried out in order to incorporate bioactive compounds into collagen-based membranes, so that such membranes could work as a drug controlled release within the target tissues [6,21]. The aim of this study was to prepare a collagen-based membranes composed by usnic acid-loaded liposomes and also to develop an easy, reproducible and accurate UV-spectrophotometric method for quantifying the usnic acid in collagen membranes, determining the main validation parameters for this complex matrix.…”

Section: Introductionmentioning

confidence: 99%

Validation of a UV-VIS Spectrophotometric method for the determination of usnic acid /collagen-based membranes

Nunes

Jesus²,

Bezerra³

et al. 2015

Scientia Plena

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O ácido úsnico (AU) é um metabólito secundário de líquens que tem demonstrado potenciais efeitos farmacológicos, tais como atividade antimicrobiana, antiviral, antiproliferativa e anti-inflamatória. O colágeno, por sua vez, é um dos biomateriais mais utilizados visando o desenvolvimento de sistema de liberação de fármacos. Sendo assim, o objetivo deste estudo foi desenvolver e validar um método analítico por espectrofotometria na região do ultravioleta (UV) para determinação de concentrações de AU em membranas de colágeno. Os parâmetros de validação foram determinados de acordo com a Agência Nacional de Vigilância Sanitária (ANVISA). A especificidade revelou que os excipientes na formulação não interferiram na análise. A linearidade nas concentrações de 2-10 µg/mL apresentou um coeficiente de correlação de 0,9994. O método mostrou excelente repetibilidade (desvio-padrão relativo < 1,0%). A precisão revelou uma recuperação média percentual de 100,43%. O método foi robusto para a variação de temperatura e solvente. Os limites de detecção e de quantificação foram 0,109 e 0,364 g/mL, respectivamente. A taxa total de recuperação das membranas analisados apresentaram valores entre 100,4 e 83,2%.Palavras-chave: ácido úsnico, membranas de colágeno, espectrofotometria UV The usnic acid (UA) is a secondary metabolite of lichen that has shown potential pharmacological effects such as antimicrobial, antiviral, antiproliferative and anti-inflammatory activities. Collagen is one of the most useful biomaterials with various applications as drug delivery systems. The aim of this study was to develop and validate a quantitative UV spectrophotometric method for the determination of UA levels in collagen membranes. The validation parameters were assessed according to the Brazilian Health Surveillance Agency (ANVISA). The specificity revealed that the excipients in the formulation did not interfere with the analysis. The linearity in the range of 2-10 μg/mL presented a correlation coefficient of 0.9994. The method showed excellent repeatability (Relative Standard Deviation (RSD) < 1.0%). The accuracy revealed a mean percentage recovery of 100.43%. The method was robust for the variation of temperature and solvent. The detection and quantization limits were found at 0.109 and 0.364 µg/mL. The total rate recovery from the analyzed membranes showed values between 100.4 and 83.2%.

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Comparative study of bovine, porcine and avian collagens for the production of a tissue engineered dermis

Cited by 85 publications

References 55 publications

Review of Collagen I Hydrogels for Bioengineered Tissue Microenvironments: Characterization of Mechanics, Structure, and Transport

Review of Collagen I Hydrogels for Bioengineered Tissue Microenvironments: Characterization of Mechanics, Structure, and Transport

Topical Collagen-Based Biomaterials for Chronic Wounds: Rationale and Clinical Application

Validation of a UV-VIS Spectrophotometric method for the determination of usnic acid /collagen-based membranes

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