Comparative Study of Antioxidant Activity of Different Extracts and Essential Oil Compositions Obtained from Artemisia sieberi Besser using Two Different Methods

Abstract

ABSTARACTThis study deals with essential oils of the aerial parts of Artemisia sieberi Besser which was obtained by hydrodistillation (HD) and simultaneous water steam distillation-organic solvent extraction (SDE) methods and compared regard to their components. The major compounds of the oil obtained by hydrodistillation were verbenol (16.09 %) and myristicin (13.76%). However, by using SDE, E-epoxy ocimen (14.02 %) and verbenol (13.93%) were detected as the major components. SDE obtained essential oil was very higher than that of hydrodistillation method based on the yield. Antioxidant activities of various extracts from A.sieberi were also evaluated via DPPH radical scavenging assay; total phenolic compounds were also measured using Folin-ciocalteu reagent. The results exhibited that, water extract of A.sieberi has considerable antioxidant activity and it seems that this activity drops off sharply with reducing polarity of extraction solvent.

Chemical Composition and Radical Scavenging Activity of Citrus Limon Peel Essential Oil

“…Activity was measured as relative decrease absorbance at 517 nm as reaction between DPPHÿ and The oil. Antioxidant activity was evaluated with %50 (IC 50 ) 8 . A 2 ml of 0.1mM DPPHÿ methanol solution with 2 ml sample with 1, 5, 10, 50, 100, 200, 400, 600, 800 and 1000 µg ml -1 concentration with shaking.…”