Comparative Study of Anti-Anemic Effect of Some Natural Food Supplements on Rats

Abstract

In this study beetroot and eggplant were used as natural source for production of anti-anemic functional food for anemic patients and to prevent it during disease or chemotherapy treatment. Anemic model was induced in albino female rats by daily injection with phenylhydrazin (5mg/kg b.w) for three successive days, then biochemical, hematological and histological parameters were determined including; iron, ferritin, Ascorbic acid, Hepicidin, Hemoglobin and Complete blood count (men corpuscle volume, men corpuscle hemoglobin, haematocriet, red blood cells and white blood cells). Negative control group of normal rats, and positive control group of anemic rats each of six rats were fed on basal diet. Also 24 anemic rats were divided into 4 groups each of 6 rats which were fed on basal diet containing either eggplant or beetroot in ratios of 100, 300g/kg, in addition to 500mg ascorbic acid and 2mg folic acid/ kg diet for all groups for 30 days. feeding period was 30 days. At the end of experiment the above biochemical and haematological parameters were evaluated and the liver and spleen were histopathological examined. Decreases in hemoglobin, men corpuscle volume, haematocriet, red blood cells, men corpuscle hemoglobin, iron, ascorbic acid and ferritin, also increases in white blood cells and hepcidin were observed in anemic model rats. At the end of feeding period with the previous supplemented tested basal diet, increases in all aforementioned parameters, except white blood cells and hepcidin which showed decreases compared to anemic positive group were found.

Petroselinum crispum L., essential oil as promising source of bioactive compounds, antioxidant, antimicrobial activities: In vitro and in silico predictions

“…The diverse chemical variation observed in parsley essential oil could be attributed to genetic and environmental factors affecting the plant's secondary metabolism, the timing of harvesting at different developmental stages, interactions with microorganisms and insects, and various post-harvest techniques, as suggested by previous research [ 36 ]. Additionally, the aging of parsley seeds led to a reduction in the concentration of various compounds, such as apiole [ 13 ].…”

Evaluation of Haemopocitic Activity of Crotalaria juncea Seed Powder Suspension in Phenylhydrazine Induced Haemolytic Anaemia in Rats

“…2.8 Blood parameter estimation (4,10,11) Under diethyl ether anaesthesia, blood was drawn from the retro-orbital sinus prior to the first injection of PHZ and on day 3, 7, 14, and 21. Blood was analyzed for red blood cells, haemoglobin, mean corpuscular volume, clotting time and haematocrit (HCT) levels.…”

“…After blood collection on last day, animals were sacrificed to isolate heart, liver, kidney, and lung (11,12) . The collected tissues were fixed immediately in 10% formalin and sent for histopathological study to Koushik laboratory and clinic, Bengaluru.…”

“…HCT is the ratio of the packed RBC volume to the total blood volume. An anaemic state is indicated by a low HCT (10)(11)(12) . Because of phenyl hydrazine-induced haemolysis, there was a considerable drop in HCT in the current study's anaemic control rats, which is consistent with other results.…”

“…It is well-known that PHZ raises MCV (Mean Corpuscular Volume), MCH (Mean Corpuscular Haemoglobin) and MCHC (Mean Corpuscular Haemoglobin Concentration) levels while lowering Hb and RBC levels (4,(10)(11)(12) . C. juncea significantly lowered this rise in MCV.…”