Comparative study of alkaline phosphatase activity in lymphocytes, mitogen-induced blasts, lymphoblastoid cell lines, acute myeloid leukemia, and chronic lymphatic leukemia cells.

Neumann, Hava; Klein, Eva; Hauck-Granoth, Roni; Yachnin, Stanley; Ben‐Bassat, Hannah

doi:10.1073/pnas.73.5.1432

Cited by 40 publications

(26 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The mean value of the ratios of the velocities, R , towards the two substrates, p-NPP and CASP, was 1.63, SD 0.5. Similar results have been reported previously (Moran et al, rg73a, b;Neumann et al, 1974). The distributions of the enzyme levels, both the the total APase and the percentage of N-APase are given in Table VII. Infectious mononucleosis.…”

Section: H Neumann Et Aisupporting

confidence: 89%

N‐Alkaline Phosphatase: a Potential Disease Marker for Lymphoproliferative Disorders

Neumann¹,

Klein²,

Russell³

et al. 1979

Br J Haematol

View full text Add to dashboard Cite

A characteristic alkaline phosphatase (orthophosphoric monoester hydrolase, alkaline pH optimum, EC 3.1.3.1) was detected in the sera of most patients with infectious mononucleosis, acute and chronic lymphatic leukaemia, non-Hodgkin's lymphoma, Burkitt's lymphoma and nasopharyngeal carcinoma. The enzyme was also present in the sera of nine out of 26 patients with cancer of the cervix. N-APase in these cases counted 30-100% of the total alkaline phosphatase activity. N-APase was absent from the sera of healthy individuals and of patients with acute and chronic granulocytic leukaemia, breast cancer, colon cancer, rheumatoid arthritis, ulcerative colitis, systemic lupus erythematosis, hepatitis and obstructive jaundice. Only three of 22 patients with Hodgkin's disease showed n-apase activity in the serum. In infectious mononucleosis the presence of N-APase activity was well correlated with the clinical course. In 13 cases studied, the clinical improvement was associated with the decrease or disappearance of N-APase activity. N-APase activity could not be detected in white cells of acute myeloid leukaemic patients, nor in the cells of myeloid blastic crisis of chronic granulocytic leukaemia. It was present in the cells of lymphoid blastic crisis of chronic granulocytic leukaemia.

show abstract

Section: H Neumann Et Aisupporting

confidence: 89%

N‐Alkaline Phosphatase: a Potential Disease Marker for Lymphoproliferative Disorders

Neumann¹,

Klein²,

Russell³

et al. 1979

Br J Haematol

View full text Add to dashboard Cite

show abstract

“…Of 6 tumor cell lines examined initially, we found high levels of AP activity in only 1. Other studies have shown high levels of this enzyme in several mouse [1] and human [6,7] hematopoietic tumor cell lines of B cell origin or osteosarcoma cell lines [2,8]. AP activity previously allowed assessment of growth inhibition in a mouse system [5],…”

Section: Discussionmentioning

confidence: 99%

Inhibition of Tumor Cell Line Alkaline Phosphatase by Human Peripheral Blood Monocytes

Schwartz

Hoffman

1984

Int Arch Allergy Immunol

View full text Add to dashboard Cite

Alkaline phosphatase (AP) activity in intact Ag8 cells was measured by a colorimetric assay in which spectrophotometer readings were obtained directly through wells of microtiter plates using a Multiskan ELISA reader. The wide difference between enzymatic activity observed in cells of this cell line and human peripheral blood monocytes permitted an assessment of inhibition of cell line growth in the presence of plastic-adherent peripheral blood cells. Comparison at 24 h of incubation of enzymatic measurements with those obtained using ³H-thymidine uptake showed comparable results, although values obtained with the enzyme were generally lower. Similarly, direct comparison of results obtained by the enzyme-based method showed good correlation with the number of viable tumor cells remaining in culture. Inhibition of AP activity was observed earlier than inhibition of ³H-thymidine incorporation, reaching its peak after 10 h of incubation. This technique has potential application for other enzyme systems and provides a new tool for analyzing mechanisms whereby effector cells are able to control proliferation of transformed cells.

show abstract

“…The cells were homogenized in the same buffer, and the Tris-C1 soluble protein fraction was separated by ultracentrifugation and denoted 45-T, 230-T and 241-T. The pellets were resuspended in water, and alkaline phosphatase activity was extracted by n-butanol: water (23% v/v) as described earlier (Neumann et al, 1976). The butanol: water extracts were denoted 45-B, 230-B and 241-B.…”

Section: Extraction and Assay Of Alkaline Phosphatase Activitymentioning

confidence: 99%

“…They were assayed for alkaline phosphatase activity using p-NPP and CASP as substrates (Neumann et al, 1967(Neumann et al, ,1976. The amount of N-alkaline phosphatase activity and the percentage were calculated according to equations previously published (Neumann et al, 1974). For the calculation ofN-alkaline phosphatase activity or its percentage the factor 1.1 was used.…”

Section: Extraction and Assay Of Alkaline Phosphatase Activitymentioning

confidence: 99%

Phenotypic Characterization of a Virus Producing Line Established from a Patient with Leukaemia/Lymphoma: Comparison with T‐Lymphoblastic and Myeloblastic Lines

Karpas¹,

Worman²,

Khalid³

et al. 1980

Br J Haematol

View full text Add to dashboard Cite

The properties have been studied of a haemic cell line established from a patient with longstanding non-Hodgkin's lymphoma after transformation to acute leukaemia. The cells are EBNA, Fc, C3 and SmIg and Ia-like antigen negative. The continuous in vitro proliferation and karyotype abnormalities suggest malignancy. The cells contain only the lymphoid type of alkaline phosphatase and a high level of terminal deoxynucleotidyl transferase and as they formed E-rosettes during the first year in culture they may be of T-cell lineage. Since the patients' leukaemic cells have not been studied it is impossible to know whether the cultured cells represent an in vitro proliferation of the patient's malignant cells or an outgrowth of a subpopulation of cells. In the cytoplasm of many cells inclusion bodies containing virus-like particles, similar to those observed in fresh human leukaemia cells, were often seen. As this new virus is biologically active, infection of the cells by virus may explain their continuous proliferation in vitro.

show abstract

Comparative study of alkaline phosphatase activity in lymphocytes, mitogen-induced blasts, lymphoblastoid cell lines, acute myeloid leukemia, and chronic lymphatic leukemia cells.

Cited by 40 publications

References 23 publications

N‐Alkaline Phosphatase: a Potential Disease Marker for Lymphoproliferative Disorders

N‐Alkaline Phosphatase: a Potential Disease Marker for Lymphoproliferative Disorders

Inhibition of Tumor Cell Line Alkaline Phosphatase by Human Peripheral Blood Monocytes

Phenotypic Characterization of a Virus Producing Line Established from a Patient with Leukaemia/Lymphoma: Comparison with T‐Lymphoblastic and Myeloblastic Lines

Contact Info

Product

Resources

About