Comparative studies on the fermentative production of lantibiotics by staphylococci

Hörner, Thomas; Ungermann, Volker; Zähner, Hans; Fiedler, Hans‐Peter; Utz, Roland; Kellner, Roland; Jung, Günther

doi:10.1007/bf00173719

Cited by 32 publications

(18 citation statements)

References 17 publications

(16 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…All these characteristics of nisin production clearly differentiate it from that of authentic secondary metabolites (Kleinkauf et al, 1986;Martin & Liras, 1989). Almost all bacteriocins produced by lactic acid bacteria are produced during the active growth phase; further, the lantibiotics Pep 5 (Sahl & Brandis,198 l), ancovenin (Kido et al, 1983), epidermin (Horner et al, 1989) and gallidermin (Horner et al, 1990) are also synthesized during the growth phase of the producer strain.…”

Section: Uncontrolled P H Jermen Tat Ionsmentioning

confidence: 99%

“…The time indications (l), (2) and (3) correspond to the arrows in (a). The blot was hybridized to the radioactively labelled natural ancovenin (Kido et al, 1983), epidermin (Horner et al, 1989) and gallidermin (Horner et al, 1990) have been shown to decrease as soon as the maximal antibiotic titres were reached. The decrease of the nisin titre after 8 h of fermentation implies that the fermentation process would need to be stopped by then to achieve a maximum specific nisin productivity.…”

Section: Prolonged Fermentations -Functional Role Of Nisinmentioning

confidence: 99%

“…L. De Vuyst and E. J . Vandamme fully applied for epidermin and gallidermin (Horner et al, 1989(Horner et al, , 1990.…”

Section: Prolonged Fermentations -Functional Role Of Nisinmentioning

confidence: 99%

“…Information about the microbial production of the lantibiotics in batch fermentations is scarce (Sahl & Brandis, 1981 ;Kido et al, 1983 ;Horner et al, 1989Horner et al, , 1990. Nisin batch fermentations have already been described (e.g.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Influence of the carbon source on nisin production in Lactococcus lactis subsp. lactis batch fermentations

Vuyst¹,

Vandamme²

1992

Journal of General Microbiology

236

178

View full text Add to dashboard Cite

Nisin production by Lactucmcus lrrctis subsp. lactis NIZO 22186 was studied in batch fermentation using a complex medium. Nisin production showed primary metabolite kinetics: nisin biosynthesis took place during the active growth phase and completely stopped when cells entered the stationary phase. A stringent correlation could be observed between the expression of the prenisin gene (nisA) and the synthesis of the post-translationally enzymically modified and processed mature nisin peptide. Moreover, it seemed likely that Nsin had a growth control function. A physiological link is proposed between sucrose fermentation capacity and nisin production ability. Garbon soure regulation appears to be a major control mechanism for nisin production.

show abstract

Section: Uncontrolled P H Jermen Tat Ionsmentioning

confidence: 99%

Section: Prolonged Fermentations -Functional Role Of Nisinmentioning

confidence: 99%

“…L. De Vuyst and E. J . Vandamme fully applied for epidermin and gallidermin (Horner et al, 1989(Horner et al, , 1990.…”

Section: Prolonged Fermentations -Functional Role Of Nisinmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Influence of the carbon source on nisin production in Lactococcus lactis subsp. lactis batch fermentations

Vuyst¹,

Vandamme²

1992

Journal of General Microbiology

236

178

View full text Add to dashboard Cite

show abstract

“…Gallidermin is a tetracyclic, 21-amino-acid, type A lantibiotic peptide which is produced by the nonpathogenic Staphylococcus gallinarum Tü3928 (25) and shows broad-spectrum activity against important gram-positive pathogens like S. aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus pneumoniae, Streptococcus faecalis, and P. acnes (20). Gallidermin (6L-epidermin) is closely related to epidermin, which is synthesized by the class II strain S. epidermidis Tü3298 (1,51).…”

mentioning

confidence: 99%

Circumventing the Effect of Product Toxicity: Development of a Novel Two-Stage Production Process for the Lantibiotic Gallidermin

Valsesia

Medaglia

Held

et al. 2007

Appl Environ Microbiol

View full text Add to dashboard Cite

Lantibiotics such as gallidermin are lanthionine-containing polypeptide antibiotics produced by grampositive bacteria that might become relevant for the treatment of various infectious diseases. So far, selftoxicity has prevented the isolation of efficient overproducing strains, thus hampering their thorough investigation and preventing their exploitation in fields other than the food area. We wanted to investigate the effect of lantibiotic precursor peptides on the producing strains in order to evaluate novel strategies for the overproduction of these promising peptides. In this study, gallidermin was chosen as a representative example of the type A lantibiotics. A Staphylococcus gallinarum Tü3928 mutant, whose gene for the extracellular pregallidermin protease GdmP was replaced by a kanamycin-resistance gene, was constructed. Mass spectrometry (MS) analysis indicated that this mutant produced fully posttranslationally modified gallidermin precursors with truncated versions of the leader peptide, but not the entire leader as predicted from the gdmA sequence. In filter-on-plate assays, these truncated pregallidermins showed no toxicity against Staphylococcus gallinarum Tü3928 up to a concentration of 8 g/liter (corresponding to approximately 2.35 mM), while gallidermin produced clear inhibitory zones at concentrations as low as 0.25 g/liter (0.12 mM). We showed that the lack of toxicity is due entirely to the presence of the truncated leader, since MS as well as bioassay analysis showed that the peptides resulting from tryptic cleavage of pregallidermins and gallidermin produced by S. gallinarum Tü3928 had identical masses and approximately the same specific activity. This demonstrates that even a shortened leader sequence is sufficient to prevent the toxicity of mature gallidermin. In nonoptimized fermentations, the gdmP mutant produced pregallidermin to a 50%-higher molar titer, suggesting that the absence of self-toxicity has a beneficial effect on gallidermin production and giving a first confirmation of the suitability of the overproduction strategy.Antibiotic resistance has spread dramatically among pathogens throughout the last two decades. Of particular concern is the increasing emergence of multiresistant pathogens in health care settings. Nowadays, about 70% of nosocomial infections in the United States are resistant to at least one antibiotic (35), and in 2002, the first clinical isolate of Staphylococcus aureus with high-level resistance to vancomycin, a drug of last resort, was isolated (8). As the number of resistant pathogens continues to grow, the number of new antibiotics to fight them is declining steeply (37). Therefore, the development of new antimicrobial agents should be encouraged.Type A lantibiotics are cationic, amphiphilic peptides that are produced by a wide range of gram-positive bacteria and show bactericidal activity against other gram-positive bacteria. They show a dual mode of action at nanomolar concentrations which involves pore formation and inhibition of peptidoglycan biosynth...

show abstract

Lantibiotics

Jack¹,

Götz²,

Jung³

1997

Biotechnology

View full text Add to dashboard Cite

Comparative studies on the fermentative production of lantibiotics by staphylococci

Cited by 32 publications

References 17 publications

Influence of the carbon source on nisin production in Lactococcus lactis subsp. lactis batch fermentations

Influence of the carbon source on nisin production in Lactococcus lactis subsp. lactis batch fermentations

Circumventing the Effect of Product Toxicity: Development of a Novel Two-Stage Production Process for the Lantibiotic Gallidermin

Lantibiotics

Contact Info

Product

Resources

About