Lantibiotics

Jack, Ralph W.; Götz, Friedrich; Jung, Günther

doi:10.1002/9783527620890.ch8

Cited by 11 publications

(2 citation statements)

References 166 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The prepropeptide consists of a propeptide at the C-terminus and a leader peptide at the N-terminus. Some of the proposed functions of the leader peptide are signaling for peptide export and serving as a scaffold for assisting posttranslational modifications, including the cleavage of the prepropeptide by a peptidase (Chatterjee et al, 2005;Jack et al, 2008). The specific posttranslational modifications of paraplantaricin TC318 are introduced by enzymes encoded in the biosynthetic gene cluster.…”

Section: Genome Mining For Bacteriocin Sequences and The Analysis Of mentioning

confidence: 99%

Genome-Guided Mass Spectrometry Expedited the Discovery of Paraplantaricin TC318, a Lantibiotic Produced by Lactobacillus paraplantarum Strain Isolated From Cheese

et al. 2020

View full text Add to dashboard Cite

The quest for potent alternatives to the currently used antimicrobials is urged by health professionals, considering the rapid rise in resistance to preservatives and antibiotics among pathogens. The current study was initiated to search for novel and effective bacteriocins from food microbes, preferably lactic acid bacteria (LAB), for potential use as preservatives. Advances in genome-guided mass spectrometry (MS) were implemented to expedite identifying and elucidating the structure of the recovered antimicrobial agent. A LAB strain, OSY-TC318, was isolated from a Turkish cheese, and the crude extract of the cultured strain inhibited the growth of various pathogenic and spoilage bacteria such as Bacillus cereus, Clostridium sporogenes, Enterococcus faecalis, Listeria monocytogenes, Salmonella enterica ser. Typhimurium, and Staphylococcus aureus. The antimicrobial producer was identified as Lactobacillus paraplantarum using MS biotyping and genomic analysis. Additionally, L. paraplantarum OSY-TC318 was distinguished from closely related strains using comparative genomic analysis. Based on in silico analysis, the genome of the new strain contained a complete lantibiotic biosynthetic gene cluster, encoding a novel lantibiotic that was designated as paraplantaricin TC318. The bioinformatic analysis of the gene cluster led to the prediction of the biosynthetic pathway, amino acid sequence, and theoretical molecular mass of paraplantaricin TC318. To verify the genomic analysis predictions, paraplantaricin TC318 was purified from the producer cellular crude extract using liquid chromatography, followed by structural elucidation using Fourier transform ion cyclotron resonance MS analysis. This genome-guided MS analysis revealed that the molecular mass of paraplantaricin TC318 is 2,263.900 Da, its chemical formula is

show abstract

Section: Genome Mining For Bacteriocin Sequences and The Analysis Of mentioning

confidence: 99%

Genome-Guided Mass Spectrometry Expedited the Discovery of Paraplantaricin TC318, a Lantibiotic Produced by Lactobacillus paraplantarum Strain Isolated From Cheese

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Lantibiotics are a group of ribosomally synthesized antimicrobial peptides (AMPs) which undergo characteristic posttranslational modifications, leading to non-canonical amino acids such as lanthionine and dehydrated serine in the mature molecule (Jack et al, 1997). Lantibiotics have been recently classified in three classes (Class I, II, or III), based on the pathway by which maturation of the peptide occurs and the presence or absence of activity (Willey and van der Donk, 2007).…”

Section: Introductionmentioning

confidence: 99%