Comparative studies on the diversity of the edible mushroom Pleurotus eryngii: ITS sequence analysis, RAPD fingerprinting, and physiological characteristics

Ro, Hyeon-Su; Kim, Sung Soon; Ryu, Jae-San; Jeon, Che‐Ok; Lee, Tae Soo; Lee, Hyun‐Sook

doi:10.1016/j.mycres.2007.03.016

Cited by 56 publications

(35 citation statements)

References 9 publications

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“…There are a variety of DNA marker techniques, such as random amplified polymorphic sequence (RAPD) (Juchum et al, 2007;Ro et al, 2007), amplified restriction fragment polymorphism (AFLP) (Percifield, 2007;Yuan et al, 2007) and microsatellite markers (SSR) (Legesse et al, 2007;Tommasini et al, 2003). They are widely applied in species or varieties identification, evaluation of genetic diversity, but few reports are available on systematically comparative analysis of two kinds of testing approaches, such as morphological method and molecular marker method.…”

Section: Introductionmentioning

confidence: 99%

SRAP Markers and Morphological Traits Could Be Used in Test of Distinctiveness, Uniformity, and Stability (DUS) of Lettuce (Lactuca sativa) Varieties

Liu¹,

Liu²,

Chen³

et al. 2011

JAS

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The test of distinctiveness, uniformity, and stability (DUS) is a necessary step for variety identification and new variety application. The objective of this study is to provide molecular marker-assisted approach combined with morphological trait-based testing for more convenient and fast DUS test and identification of varieties. Eighteen pairs of SRAP markers and 40 morphological traits for DUS test were applied for genetic diversity analysis of 50 lettuce (Lactuca sativa) varieties. Average polymorphism information content (PIC) of the SRAP markers was 0.80, ranging from 0.39 to 0.97. Cluster analysis using UPGMA of the band patterns amplified by SRAP marker and morphological trait-based clustering separated the varieties into three groups. The correlation coefficient of SRAP marker and morphological traits was 0.5455 reflecting that the two clustering results shared some similarity and consistence. It revealed that the combination of both SRAP marker and morphological trait analysis is more conducive to proper identification and classification of plant varieties, which will undoubtedly bring an alternative choice to DUS testing of plant new varieties and conservation of plant germplasm.

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Section: Introductionmentioning

confidence: 99%

SRAP Markers and Morphological Traits Could Be Used in Test of Distinctiveness, Uniformity, and Stability (DUS) of Lettuce (Lactuca sativa) Varieties

Liu¹,

Liu²,

Chen³

et al. 2011

JAS

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“…Pleurotus eryngii is an edible mushroom with a high commercial value and has been widely cultivated in Europe, Middle East, North America, and Asia (Mandeel et al 2005). The genetic diversity and population structure of P. eryngii have been investigated in several studies (Urbanelli et al 2003(Urbanelli et al , 2007Abdollahzadeh et al 2007;Ro et al 2007;Zervakis et al 2014). However, little is known about the mitochondrial genome of this fungus.…”

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confidence: 99%

The complete mitochondrial genome of the Basidiomycete edible fungus Pleurotus eryngii

Yang

et al. 2016

Mitochondrial DNA Part B

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The complete mitochondrial genome of the edible fungus Pleurotus eryngii (oyster mushroom) was determined using Illumina sequencing. This mitogenome is a circular molecule of 72,650 bp in length with a GC content of 26.28%. Gene prediction showed that 40 putative protein-coding genes, the small ribosomal RNA subunits (rns), and 23 tRNAs were located on the same strand. The mitogenome of P. eryngii has a similar structure to that of P. ostreatus in both gene content and gene order. The mitogenome information of P. eryngii should contribute to our understanding of the diversity and evolution of Pleurotaceae and Agaricales.

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“…Thus, the RAPD analysis highlighted the difference between the species and could be applied to differentiating strains of the same species. Aiming to develop the genetic patterns obtained from RAPD techniques, there are only few others studies (such as the present one) dealing with G. lucidum (Hseu et al, 1996;Singh et al, 2005;Ro et al, 2007). The major difficulty in applying this technique in studies with systematic relies on the extreme sensitivity of RAPD.…”

Section: Resultsmentioning

confidence: 95%

“…The RAPD may not be very useful when applied to determine very elaborated phylogenies, evolving big taxonomic groups, but its results are recognized when specific groups are studied (Duncan et al, 1993;Hamelin et al, 1993;Manulis et al, 1994;Laroche et al, 1995;Gomes et al, 2009;Stringari et al, 2009;Gonçalves et al, 2010). Ro et al (2007) obtained good results when they used RAPD aiming to differentiate the strains of Pleurotus eryngii. The technique has been used in the studies with Verticillium fungicola, with excellent results (Del Carmen et al, 2002; and by other researchers for works with many other organisms, always differentiating very close taxonomic groups (Grazziotin et al, 2005;Muok et al, 2007;Tang et al, 2007;Rajwana et al, 2008;Solouki et al, 2008;Venkatachalam et al, 2008;Tsvelikas et al, 2009).…”

Section: Resultsmentioning

confidence: 99%

“…These markers have the advantage of amplifying both regions of the genome which may be transcript/translated, and non-coding regions. This is important when the objective is to evaluate the variation along the biggest part of the species genome (Williams et al, 1990;Ferreira and Grattapaglia, 1996;Ro et al, 2007). On the other hand, RAPD has limitations for its low experimental reproducibility, although this problem may be overcome with the use of many primers and with more strict criteria in analyzing the results (Carvalho and Vieira, 2001).…”

Section: Introductionmentioning

confidence: 99%

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Use of RAPD molecular markers on differentiation of brazilian and chinese Ganoderma lucidum strains

Rolim

Cavalcante

Urben

et al. 2011

Braz. arch. biol. technol.

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Comparative studies on the diversity of the edible mushroom Pleurotus eryngii: ITS sequence analysis, RAPD fingerprinting, and physiological characteristics

Cited by 56 publications

References 9 publications

SRAP Markers and Morphological Traits Could Be Used in Test of Distinctiveness, Uniformity, and Stability (DUS) of Lettuce (Lactuca sativa) Varieties

SRAP Markers and Morphological Traits Could Be Used in Test of Distinctiveness, Uniformity, and Stability (DUS) of Lettuce (Lactuca sativa) Varieties

The complete mitochondrial genome of the Basidiomycete edible fungus Pleurotus eryngii

Use of RAPD molecular markers on differentiation of brazilian and chinese Ganoderma lucidum strains

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