Comparative Studies on Sorption Recovery and Molecular Selectivity of Bondesil PPL versus Bond Elut PPL Sorbents with Regard to Fulvic Acids

Abstract

Large scale isolation—in gram quantities—of dissolved organic matter (DOM) from natural waters is necessary for detailed investigation of its role in chemical and microbial processes driving carbon cycling under conditions of global climate change. The best candidate for a use in these large-scale experiments is a bulk sorbent Bondesil PPL, which has the same modification as the widely used Bond Elut PPL sorbent. There have been no studies so far reported on interchangeability of these sorbents with regard to DOM isolation. This work was devoted to comparative studies on sorption efficiency and molecular selectivity of these two sorbents—Bond Elut PPL and Bondesil PPL with regard to DOM components. Fulvic acids (FA) from peat water leachate were used as a model DOM. Laboratory solid phase extraction (SPE) setup was used for monitoring sorption recovery and extraction yield. It included three parallel experiments on pre-packed Bond Elut PPL cartridges (500 mg/3 mL) and three self-packed Bondesil PPL cartridges (500 mg/3 mL). Fourier transform ion cyclotron resonance mass spectrometry (FT ICR MS) and 13C/1H nuclear magnetic resonance (NMR) spectroscopy were used for determination of molecular and structural group compositions of the FA isolates obtained with a use of two different sorbents. The results of this study allowed a conclusion on interchangeability of the two sorbents used in this study for the purposes of DOM isolation from natural waters. This conclusion was backed up by similarity of sorption behavior of the peat FA components on both sorbents and by high similarity of molecular compositions and carbon distribution among the main structural groups.

Solid-Phase Extraction at High pH as a Promising Tool for Targeted Isolation of Biologically Active Fractions of Humic Acids

“…Ultrahigh-resolution mass spectra were acquired on a Bruker solariX 15 T FT ICR mass spectrometer (Bruker Daltonics, Bremen, Germany) equipped with a 15 T superconducting magnet and an Apollo II source located at the user facilities of the Zelinski Institute of Organic Chemistry of RAS. The acquisition conditions applied in this study followed those described in refs , , and . The molecular formulas assignments were made using lab-made Transhumus software developed by Anton Grigoriev.…”

“…The molecular formulas assignments were made using lab-made Transhumus software developed by Anton Grigoriev. The mass accuracy window was set at a value of <0.5 ppm (Da/MDa), and the chemical constraints were used as described in refs and . The obtained molecular assignments were used for calculation of H/C and O/C ratios, which were plotted as Van Krevelen diagrams represented by a relationship of H/C versus O/C values as described by Kim et al The quantitative treatment of the Van Krevelen diagrams was conducted using a cell-partitioning algorithm as described by Perminova .…”

“…Fractionation is one of the most effective approaches, which allows for the reduction of chemical and isomeric diversity of HS as well as the extraction of the more biologically active components. − Solid-phase extraction (SPE) with the use of prepacked cartridges (e.g., Bond Elute PPL) is widely used as a method of preparative extraction of HS from aquatic environments. − SPE, in combination with gradient elution with solvents differing in polarity, was effective in both the separation of molecular components and the isolation of fractions with increased inhibitory activity against β-lactamase TEM. , …”

Yedoma Permafrost Releases Organic Matter with Lesser Affinity for Cu2+ and Ni2+ as Compared to Peat from the Non-Permafrost Area: Risk of Rising Toxicity of Potentially Toxic Elements in the Arctic Ocean

“…For the NMR study, 40 mg of HA was dissolved in 1 mL of NaOD in 40 wt. % in D 2 O, 99.5 atom % D (Sigma Aldrich, Germany), and 13 C NMR spectra were recorded according to the conditions described in [42,43]. To exclude the nuclear Overhauser effect, the INVGATE pulse technique was used.…”