Comparative Studies on Ovine and Bovine C-Type Particles1

Bovine and ovine leukemia viruses. I. Characterization of viral antigens

“…PAGE of viral proteins. A comparative previously identified by radioimmunoprecipitaanalysis of pmteins from purified ovine and botion of [35S]methionine-labeled virus preparavine leukemia viruses showed that major differ-tions (18). Line II, which was formed by the ences of protein composition are not obvious "ether-sensitive" antigen (14,17), consisted of ( Fig.…”

“…dioimmunoprecipitation. With [3S]methionine-labeled antigen preparations, we have pre-DISCUSSION viously demonstrated that one major antigen, p21, is precipitated by homologous and heterol-OLV and BLV are C-type RNA tumor viruses ogous sera from both OLV and BLV (18). Imthat share several cross-reacting antigens (20) munoprecipitation of [3H]glucosamine-labeled and that cannot be distinguished morphologiviral proteins of ovine and bovine origin, folcally (13 32,000 (gp32), respectively.…”

“…One major [6-3H]glucosamine hydrochloride (19 Ci/mmol) per antigen (p21) was found to be indigenous to both ml. Medium was collected after 16 h, and virus was viruses .. To further elucidconcentrated by centrifugation and purified by isopycviruses (18). o furtner elucidate this relation-nic banding as described above.…”

Progress in Studies on the Etiology and Serologic Diagnosis of Enzootic Bovine Leukosis

“…Proteins pIS, p24, and gp69 were purified to electrophoretic homogeneity and were shown to react with specific antibodies from leukemic cattle (Kaaden et aI., 1976(Kaaden et aI., ,1977Frenzel et aI., 1977;Kaaden, in press). Conclusive proof for the virus specificity of the remaining Gilden et aI., 1975;Kettmann et aI., 1975. There are no specifications reported concerning the protein structure of the enzyme * An p21 antigen was also identified by Paulsen et al (1976) in both bovine and ovine leukemia viruses by SDS-PAGE and indirect radioimmune precipitation polypeptides so far has not been obtained. , 1977).…”

Location of antigens associated with bovine leukemia virus

“…An internal antigen (6,13) of BLV has been reported with various molecular weights (M. W.) of 21,000 (15), 23,000 (14), 24,000 (3), and 25,000 (5). This antigen is ether resistant and will be referred to as p23 (14).…”