Location of antigens associated with bovine leukemia virus

Abstract

Antigens associated with bovine leukemia virus (BLV) infection (gp 44/55 and p23) were identified by neutralization and immunofluorescent antibody (FA) tests. Only serums containing antibody to gp 45/55 neutralized the virus; antibody to p23 did not. These results indicate that gp 44/55 may be located in the virus envelope. In indirect FA tests, both a cytoplasmic fluorescence and a membrane fluorscence were detected in BLV-infected cells. The serum used in the FA test was then absorbed with either p23 or gp 45/55. The results of these absorption tests indicate that the membrane fluorescence maybe associated with gp 45/55 and the cytoplasmic fluorescence may be associated with p23.

Induction of Transformed Phenotypes in Sheep Fibroblasts by Culture Fluids from Cells Persistently Infected with Bovine Leukemia Virus

“…Membrane fluorescent antibody (MFA) test. An indirect MFA test was performed to detect BL V -related membrane antigen on the cell surface of viable cells (13). Monolayer cells were dispersed, incubated with the reference V34 or S-28 serum and then stained with fluorescein isothiocyanate-cojugated anti-sheep y-globulin rabbit serum.…”