Comparative Studies of Oleaginous Fungal Strains (<i>Mucor circinelloides</i> and <i>Trichoderma reesei</i>) for Effective Wastewater Treatment and Bio-Oil Production

Bhanja, Anshuman; Minde, Gauri P.; Magdum, Sandip S.; Kalyanraman, V.

doi:10.1155/2014/479370

Cited by 26 publications

(12 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This process is dependent on ER alkaneinducible cytochrome P450 in T. harzianum (507). The ability to metabolize alkanes in an industrial context is attractive and would allow the production of value-added products from contaminated substrates, as has been explored using an oleaginous strain of T. reesei (508).…”

Section: Lipid Metabolismmentioning

confidence: 99%

The Genomes of Three Uneven Siblings: Footprints of the Lifestyles of Three Trichoderma Species

Schmoll

Dattenböck

Carreras-Villaseñor

et al. 2016

Microbiol Mol Biol Rev

163

195

View full text Add to dashboard Cite

SUMMARYThe genusTrichodermacontains fungi with high relevance for humans, with applications in enzyme production for plant cell wall degradation and use in biocontrol. Here, we provide a broad, comprehensive overview of the genomic content of these species for “hot topic” research aspects, including CAZymes, transport, transcription factors, and development, along with a detailed analysis and annotation of less-studied topics, such as signal transduction, genome integrity, chromatin, photobiology, or lipid, sulfur, and nitrogen metabolism inT. reesei,T. atroviride, andT. virens, and we open up new perspectives to those topics discussed previously. In total, we covered more than 2,000 of the predicted 9,000 to 11,000 genes of eachTrichodermaspecies discussed, which is >20% of the respective gene content. Additionally, we considered available transcriptome data for the annotated genes. Highlights of our analyses include overall carbohydrate cleavage preferences due to the different genomic contents and regulation of the respective genes. We found light regulation of many sulfur metabolic genes. Additionally, a new Golgi 1,2-mannosidase likely involved inN-linked glycosylation was detected, as were indications for the ability ofTrichodermaspp. to generate hybrid galactose-containingN-linked glycans. The genomic inventory of effector proteins revealed numerous compounds unique toTrichoderma, and these warrant further investigation. We found interesting expansions in theTrichodermagenus in several signaling pathways, such as G-protein-coupled receptors, RAS GTPases, and casein kinases. A particularly interesting feature absolutely unique toT. atrovirideis the duplication of the alternative sulfur amino acid synthesis pathway.

show abstract

Section: Lipid Metabolismmentioning

confidence: 99%

The Genomes of Three Uneven Siblings: Footprints of the Lifestyles of Three Trichoderma Species

Schmoll

Dattenböck

Carreras-Villaseñor

et al. 2016

Microbiol Mol Biol Rev

163

195

View full text Add to dashboard Cite

show abstract

“…T. reesei had a yield of 28.7% with a dry cell weight (DCW) of 14.60 g/L comparable to a report where T. reesei isolated from wood could produce 30 g/L and 32.4% of DCW (Bharathiraja et al, 2017). The potential of strains of M. circinelloides and T. reesei as oleaginous fungi and feedstock for biodiesel production has been widely reported (Bhanja et al, 2014;Qaio et al, 2018;Zhao et al, 2018;Kumar et al, 2014;Bharathiraja et al, 2017).…”

Section: Discussionmentioning

confidence: 56%

FAMEs Profile of Oil Produced by Oleaginous Fungi Isolated from Fermented Beverage Wastewaters and Soil

Nsa¹,

Adeloye²,

Odunsi³

et al. 2020

Nig. J. Biotechnol

View full text Add to dashboard Cite

Fungal strains isolated from fermented maize (ogi) (PW) and sorghum-based brewery wastewaters (BW) and two soil isolates were evaluated for oleaginicity. The fungal isolates from the wastewater that had lipid content of at least 20% of their biomass were identified by both culture methods and internal transcribed spacer (ITS) 1-5.8S-ITS2 ribosomal DNA sequencing. The isolates were identified to be Aspergillus fumigatus (PW8), Aspergillus flavus (PW10), Candida tropicalis (PW16) and Aspergillus tubingensis (PW3), Trichosporon luoberi (BW7), Aspergillus sp. (BW4) and Candida tropicalis (BW1; BW3). FAMEs composition was determined for the four strains with the highest lipid content by acid-catalyzed transesterification and analyzed by Gas Chromatography-Flame Ionization Detector (GC-FID). Palmitoleic acid was the dominant fatty acid in M. circinelloides and T. reesei, and the best producers of capric and lauric acids were Aspergillus fumigatus and Aspergillus sp. (BW4), respectively. These fatty acids are beneficial in making cosmetics and pharmaceuticals (antimicrobials and dietary supplements). The analysis of the FAMEs profile in the species indicated low amounts or absence of some key long chain fatty acid (LCFA) constituents of biodiesels. Based on the FAMEs profile of M. circinelloides investigated, this strain could hold promise for use as feedstock for biodiesel with genetic engineering and a tailored lipid production favouring enrichment of LCFA. Keywords: Fungal lipids, wastewater, fatty acid methyl ester, GC-FID

show abstract

“…Concerning Mucor circinelloides oil (MU), it displayed a very low percent of triglycerides, with a very high amount of FFAs, monoglycerides and diglycerides (Table ). Although these properties position MU oil as an inadequate raw material for biodiesel synthesis, previous assays using alkaline transesterification reported high conversion values and a high biodiesel quality . Interestingly, during the identification of the mass spectroscopy ions of MU oil, an unusual unsaturated 18:X acyl glyceride, not yet identified, was detected as free, mono and diglyceride, showing a relevant peak area (Supporting Information Table S3).…”

Section: Resultsmentioning

confidence: 97%

“…During the last years, strains of the genus Mucor have gained attention because of their high contents in lipids and the good growth rate of the strains . This positions the oils produced by Mucor yeasts as interesting potential biofuel feedstocks, and specifically Mucor circinelloides oil, which is already being studied for transesterification in biodiesel production . Among other nonfood, alternative oils for biofuel production are the waste cooking oils .…”

Section: Introductionmentioning

confidence: 99%

Alternative Oils Tested as Feedstocks for Enzymatic FAMEs Synthesis: Toward a More Sustainable Process

Infanzón

Cesarini

Martínez

et al. 2017

Biotechnology Progress

View full text Add to dashboard Cite

Previously isolated and characterized Pseudomonas lipases were immobilized in a low-cost MP-1000 support by a re-loading procedure that allowed a high activity per weight of support. Immobilized LipA, LipC, and LipCmut lipases, and commercial Novozym® 435 were tested for fatty acid methyl ester (FAMEs) synthesis using conventional and alternative feedstocks. Triolein and degummed soybean oils were used as model substrates, whereas waste cooking oil and M. circinelloides oil were assayed as alternative, low cost feedstocks, whose free fatty acid (FFA), and acylglyceride profile was characterized. The reaction conditions for FAMEs synthesis were initially established using degummed soybean oil, setting up the best water and methanol concentrations for optimum conversion. These conditions were further applied to the alternative feedstocks and the four lipases. The results revealed that Pseudomonas lipases were unable to use the FFAs, displaying a moderate FAMEs synthesis, whereas a 44% FAMEs production was obtained when M. circinelloides oil was used as a substrate in the reaction catalysed by Novozym® 435, used under the conditions established for degummed soybean oil. However, when Novozym® 435 was tested under previously described optimal conditions for this lipase, promising values of 85 and 76% FAMEs synthesis were obtained for waste cooking oil and M. circinelloides oil, respectively, which might result in promising, nonfood, alternative feedstocks for enzymatic biodiesel production. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 33:1209-1217, 2017.

show abstract

Comparative Studies of Oleaginous Fungal Strains (Mucor circinelloides and Trichoderma reesei) for Effective Wastewater Treatment and Bio-Oil Production

Cited by 26 publications

References 30 publications

The Genomes of Three Uneven Siblings: Footprints of the Lifestyles of Three Trichoderma Species

The Genomes of Three Uneven Siblings: Footprints of the Lifestyles of Three Trichoderma Species

FAMEs Profile of Oil Produced by Oleaginous Fungi Isolated from Fermented Beverage Wastewaters and Soil

Alternative Oils Tested as Feedstocks for Enzymatic FAMEs Synthesis: Toward a More Sustainable Process

Contact Info

Product

Resources

About