Alternative Oils Tested as Feedstocks for Enzymatic FAMEs Synthesis: Toward a More Sustainable Process

Abstract

Previously isolated and characterized Pseudomonas lipases were immobilized in a low-cost MP-1000 support by a re-loading procedure that allowed a high activity per weight of support. Immobilized LipA, LipC, and LipCmut lipases, and commercial Novozym® 435 were tested for fatty acid methyl ester (FAMEs) synthesis using conventional and alternative feedstocks. Triolein and degummed soybean oils were used as model substrates, whereas waste cooking oil and M. circinelloides oil were assayed as alternative, low cost feedstocks, whose free fatty acid (FFA), and acylglyceride profile was characterized. The reaction conditions for FAMEs synthesis were initially established using degummed soybean oil, setting up the best water and methanol concentrations for optimum conversion. These conditions were further applied to the alternative feedstocks and the four lipases. The results revealed that Pseudomonas lipases were unable to use the FFAs, displaying a moderate FAMEs synthesis, whereas a 44% FAMEs production was obtained when M. circinelloides oil was used as a substrate in the reaction catalysed by Novozym® 435, used under the conditions established for degummed soybean oil. However, when Novozym® 435 was tested under previously described optimal conditions for this lipase, promising values of 85 and 76% FAMEs synthesis were obtained for waste cooking oil and M. circinelloides oil, respectively, which might result in promising, nonfood, alternative feedstocks for enzymatic biodiesel production. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 33:1209-1217, 2017.

Novozym 435: the “perfect” lipase immobilized biocatalyst?

“…461 N435 gave promising values of 85 and 76% of biodiesel from waste cooking oil and M. circinelloides oil, respectively. 462 Fish oil was transformed into biodiesel and enriched in polyunsaturated fatty acids by methanolysis catalyzed by NS 81006 followed by hydrolysis by N435. 463 Different lipases were compared in biodiesel production using sunflower oil and methanol.…”

Liquid lipase‐catalyzed hydrolysis of gac oil for fatty acid production: Optimization using response surface methodology

“…Fatty acids have attracted considerable attention due to their various industrial applications such as in manufacturing surfactants, soaps, food products, and various biomedical applications . In addition, the conversion of fatty acids into biodiesel through esterification with alcohol has gained the interest of researchers worldwide as biodiesel is considered to be an alternative to petrodiesel to solve the shortage of fossil fuels and environmental concerns . Therefore, those increasing interests have motivated the development of efficient methods for the production of fatty acids.…”