Comparative structural analysis of fruit and stem bromelain from Ananas comosus

Ramli, Aizi Nor Mazila; Manas, Nor Hasmaliana Abdul; Hamid, Azzmer Azzar Abdul; Hamid, Hazrulrizawati Abd; Illias, Rosli Md.

doi:10.1016/j.foodchem.2018.05.125

Cited by 41 publications

(36 citation statements)

References 49 publications

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“…The specific part of from which it is extracted lends its name to the enzyme, thus we have fruit bromelain and stem bromelain [1]. Bromelain activity has been reported to be within a pH range of 3 to 8 and a temperature range of 30-70 ºC [2][3][4].…”

Section: Introductionmentioning

confidence: 99%

Bromelain Activity of Waste Parts of Two Pineapple Varieties

Benefo

Ofosu

2018

SFP

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Bromelain, a protease found in pineapples, is of high demand in the pharmaceutical, cosmetic and food industries. Along the pineapple processing chain, waste products such as peels, crowns, stems and cores result. These parts are usually discarded, though they contain significant amounts of the enzyme bromelain. This study sought to determine the bromelain activity of the crowns and peels of two pineapple varieties grown in Ghana; MD2 and Sugarloaf. The crude extract was obtained by homogenising the peels and crowns in a cold phosphate buffer and centrifuging at 3000 rpm for 15 min. Ethanol and ammonium sulphate precipitation were carried out on the extract between 30%-80% precipitation levels. The enzyme activity was determined using the casein digestion method. Results showed that bromelain was precipitated mainly in the 30%-60% precipitation range. Sugarloaf crowns yielded the highest enzyme activity of 20.82 U/ml and a specific activity of 194.58 U/mg at the 40% ammonium sulphate precipitation level. This was followed by the Sugarloaf peels with an enzyme activity of 19.98 U/ml at 50% ethanol precipitation level. Ethanol precipitation resulted in fractions with lower bromelain activity. Enzyme activity was higher in the Sugarloaf variety and also in the crowns of both varieties. The two pineapple varieties have significant levels of bromelain activity and could be exploited for commercialisation.

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Section: Introductionmentioning

confidence: 99%

Bromelain Activity of Waste Parts of Two Pineapple Varieties

Benefo

Ofosu

2018

SFP

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“…The most intense peaks are gathered between 24,400.0 Da and 25,250.0 Da, with a maximum around 24,645.0 Da. This heterogeneity may have two origins; the heterogeneity of the amino acid sequence as previously reported 34 as well as the heterogeneity resulting from the glycosylation pattern 1 . The sample was therefore further analyzed by ESI-Q-TOF MS/MS after digestion by trypsin.…”

Section: Analysis Of the Purified Forms Of Bromelain By Mass Spectrommentioning

confidence: 84%

Structures of the free and inhibitors-bound forms of bromelain and ananain from Ananas comosus stem and in vitro study of their cytotoxicity

Azarkan

Maquoi

Delbrassine

et al. 2020

Sci Rep

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The Ananascomosus stem extract is a complex mixture containing various cysteine proteases of the C1A subfamily, such as bromelain and ananain. This mixture used for centuries in Chinese medicine, has several potential therapeutic applications as anti-cancer, anti-inflammatory and ecchymosis degradation agent. In the present work we determined the structures of bromelain and ananain, both in their free forms and in complex with the inhibitors E64 and TLCK. These structures combined with protease-substrate complexes modeling clearly identified the Glu68 as responsible for the high discrimination of bromelain in favor of substrates with positively charged residues at P2, and unveil the reasons for its weak inhibition by cystatins and E64. Our results with purified and fully active bromelain, ananain and papain show a strong reduction of cell proliferation with MDA-MB231 and A2058 cancer cell lines at a concentration of about 1 μM, control experiments clearly emphasizing the need for proteolytic activity. In contrast, while bromelain and ananain had a strong effect on the proliferation of the OCI-LY19 and HL-60 non-adherent cell lines, papain, the archetypal member of the C1A subfamily, had none. This indicates that, in this case, sequence/structure identity beyond the active site of bromelain and ananain is more important than substrate specificity.

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“…Nanas (Ananas comosus) merupakan tanaman hortikultura tropis yang buahnya banyak manfaat karena kandungan gizi berupa kalsium, potassium, vitamin, bromealin, dan serat (Harahap & Nusyirwan 2014;Adam et al 2016;Ramli et al 2018). Nanas tidak hanya dikonsumsi buahnya, tetapi bagian tanaman lainnya, seperti daun dan limbahnya dapat dimanfaatkan.…”

Section: Pendahuluanunclassified

Keanekaragaman Mikrob Fungsional Rizosfer Nanas dengan Berbagai Tingkat Produktivitas

Utami¹,

Wiyono²,

Widyastuti³

et al. 2020

J. Ilmu. Pertan. Indones.

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Functional microbes of rhizosphere play important roles in nutrient transformation and controlling disease as well as in supporting plant growth and development. However, there is no study on the role of functional microbes on pineapple productivity. The purpose of this study was to investigate the abundance and diversity of soil functional microbes at different growth phases at two levels of productivity and their correlations to disease incidence. The research process included sampling of pineapple rhizospheric soil from vegetative and generative phases pineapples at low and high plant productivity sites, observations of disease incidence, and isolations of functional microbes. Functional groups of bacteria were Azotobacter, phosphate-solubilizing bacteria, potassium-solubilizing bacteria, antibiotics-producing bacteria, IAA-producing bacteria, and chitinolytic bacteria. The soil sampling method was simple randomized sampling at 6 locations with an area of each location ± 5 ha with a depth of 20 cm. Rhizosphere were taken in plants grown in high productivity area (>60tons/ha) and low productivity area (<60 tons/ha) in vegetative and generative phases. The results showed that potassium-solubilizing bacteria, chitinolytic bacteria, and IAA-producing bacteria were more abundant during the generative phase compared to those during vegetative phase. While Azotobacter, phosphate-solubilizing bacteria, and antibiotic-producing bacteria were more predominant during vegetative phase at various crop productivy. Total density of microbes was higher in soil with high crop productivity than that in soil with low crop productivity. The abundance of chitinolytic bacteria and IAA-producing bacteria had negative correlation with disease caused by Erwinia chrysanthemi and Phytophthora cinnamomi. Keywords: chitinolytic bacteria, growth phase, IAA, pineapple disease

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Comparative structural analysis of fruit and stem bromelain from Ananas comosus

Cited by 41 publications

References 49 publications

Bromelain Activity of Waste Parts of Two Pineapple Varieties

Bromelain Activity of Waste Parts of Two Pineapple Varieties

Structures of the free and inhibitors-bound forms of bromelain and ananain from Ananas comosus stem and in vitro study of their cytotoxicity

Keanekaragaman Mikrob Fungsional Rizosfer Nanas dengan Berbagai Tingkat Produktivitas

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