Bromelain Activity of Waste Parts of Two Pineapple Varieties

Benefo, Edmund O.; Ofosu, Isaac W.

doi:10.18052/www.scipress.com/sfp.2.21

Cited by 8 publications

(7 citation statements)

References 20 publications

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“…Therefore, further characterization was done using 40% as final saturation percentage. These result lies close to the findings of Benefo and Ofosu [16] that also demonstrated 2.47-fold purification at 40% (NH4)2SO4 saturation. Bresolin and coauthors.…”

Section: Ammonium Sulphate (Nh4)2so4 Precipitation Of Pineapple Waste Parts and Its Effect On Purification Fold And Percent Recoverysupporting

confidence: 90%

Extraction, Partial Purification and Characterization of Bromelain from Pineapple (Ananas Comosus) Crown, Core and Peel Waste

Gul

Siddiqui

Arain

et al. 2021

Braz. arch. biol. technol.

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Ananas Comosus (also known as pineapple) is a part of Bromeliaceae family and it is consumed as food as well as folk medicine for the treatment of various diseases. It is reported that pineapple is a rich source of bromelain, a cysteine protease and it is considered as an important enzyme in different industries due to its significant therapeutic and industrial applications such as anticancer, anti-inflammatory and meat tenderizing. Bromelain is mostly present in fruit and stem of pineapple, but it is reported that crown, core, and peels, which constitute the waste of the pineapple plant, also contain bromelain but limited data is available. Therefore, the proposed study aimed at utilizing pineapple waste for the extraction and characterization of bromelain. Firstly, crude bromelain was extracted with phosphate buffer (pH 7), then it was subjected to partial purification using different fractions of ammonium sulphate (NH4)2SO4 such as 30, 40, 50 and 60% followed by desalting and concentration. Enzyme activity was calculated by using casein digesting unit (CDU) method. The results demonstrated that the crown bromelain showed highest purification of 4.34-fold at 30% HIGHLIGHTS• Bromelain was extracted from crown, core, and peel waste of pineapple.• Ammonium sulphate (NH4)2SO4 precipitation method was used as a simple and cost-effective approach for partial purification of bromelain.• A purification of 4.34, 2.75 and 2.59-fold was achieved for crown, core and peel bromelain samples respectively.• Enzyme activity recovery of 170.41 %, 192.37 % and 337.59 % was achieved for crown, core, and peel bromelain, respectively. 2 Gul, A., et al.

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Section: Ammonium Sulphate (Nh4)2so4 Precipitation Of Pineapple Waste Parts and Its Effect On Purification Fold And Percent Recoverysupporting

confidence: 90%

Extraction, Partial Purification and Characterization of Bromelain from Pineapple (Ananas Comosus) Crown, Core and Peel Waste

Gul

Siddiqui

Arain

et al. 2021

Braz. arch. biol. technol.

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“…Another fairly well-studied plant-derived enzyme is bromelain, which is found in large quantities in the waste (peels, crowns, stems, and cores) of the pineapple processing process. It is from the fruit crowns that bromelain is obtained with the highest activity (20.82 OD/ml) and specific activity of 194.58 OD/mg [8].…”

Section: Main Partmentioning

confidence: 97%

Correction of Exocrine Pancreatic Insufficiency by Plant-Derived Enzymes (Literature Review)

Schvets,

Bentsa,

Tkachyshyn

et al. 2023

VPBM

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Notwithstanding the complex cytoarchitecture of the CNS, high sensitivity and metabolic activity of neuronal cells, no specialized anatomical structure has been identified that would facilitate effective «lymphatic» clearance of extracellularly dissolved substances and potentially toxic metabolites. World scientific research has highlighted the concept of a glymphatic system that enables the brain to draine the extracellular harmful substances dissolved in the interstitium. At the same time, aquaporin-4 (AQP4) water channels are an important component of this system, and their dysfunction is associated with a number of neuropathologies. Despite the fact that potential AQP modulators have already been described, there are many concerns over their use as a therapeutic target due to low drug sensitivity.It is well known that aging is a critical risk factor for neurodegenerative diseases. It has also been experimentally proven that glymphatic activity decreases with aging. Accordingly, this can lead to the accumulation of misfolded and hyperphosphorylated proteins, and thus the brain becomes vulnerable to the development of neurodegenerative pathology. Abnormal expansion of the perivascular space is more commonly observed in Alzheimer's disease in elderly subjects, indicating a possible deformation of the glymphatic pathways and a subsequent decrease in protein clearance.Current investigations are focused as well on clarifying changes in brain glymphatic drainage in the condition of traumatic brain injury. Modern research has shown that acute brain injury, including traumatic brain injury, subarachnoid hemorrhage, or stroke, dramatically alters glymphatic function and impairs convective fluid flow. It has also been experimentally confirmed that moderate traumatic brain injury is characterized by a prolonged decrease in glymphatic function, which increases the risk of developing chronic traumatic encephalopathy.Comprehensive analysis of the causes and mechanisms of glymphatic system dysfunction will help to predict and develop methods for diagnosing and treating serious neurodegenerative diseases and traumatic brain injuries.

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“…The reasons for its popularity include its easy operation, reproducibility, and minimum equipment requirements. In addition, ethanol is an inexpensive organic solvent produced widely in Brazil and is recoverable using a simple distillation process [66].…”

Section: Ethanol Precipitationmentioning

confidence: 99%

Biochemical Characteristics and Healing Activity of Bromelia laciniosa Leaf Protease

Silva¹,

Nerys²,

Silva³

et al. 2022

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Macambira plant (Bromelia laciniosa Mart. Ex Shult. & Schult. f.) is a species belonging to the family Bromeliaceae, which is distributed widely in the hinterlands of northeastern Brazil, from Bahia to Piauí states. The plants of this species contain an enzyme complex named bromelain, which has great potential for therapeutic applications. In the present study, bromelain derived from Bromelia laciniosa leaves was assessed for its thermodynamic properties and in vitro cytotoxic and healing activities. Bromelain was extracted from the leaves and partitioned using ethanol. The partially purified bromelain was then characterized to determine the optimum pH and temperature and the kinetic and thermodynamic parameters. The partially purified bromelain derived from macambira leaves exhibited its highest activity at 47°C and pH 7, when the Km and Vmax values of 2.26 µM and 2.083 U/mL, respectively. The half-life of this bromelain at 47°C was 5.78 h. At 47°C, the Gibbs's free energy, enthalpy, and entropy values reached 89, 53.54, and –0.11 kJ/mol K, respectively. In the SDS-PAGE analysis, bromelain appeared as the main protein band, with an estimated molecular mass of 33 kDa. The in vitro cytotoxic activity and wound healing effects were determined using the MTT assay and Scratch assay, respectively. These assays were performed on the non-neoplastic cell lines L-929 and PBMC and the human tumor cell lines MCF-7, HCT-116, and NCI-H292. Bromelain exhibited a positive healing activity with a change rate similar to that observed for the control. In addition, bromelain exhibited cytotoxic activity against NCI-H292 tumor cells and chemopreventive potential. These results indicate that bromelain derived from macambira leaves may be utilized as a potential ingredient in pharmaceutical formulations, for various healing and chemopreventive purposes and as an alternative to the bromelain extracted from pineapple. In addition, the biochemical characteristics of this bromelain could find application in the food industry. Future studies should, therefore, focus on deciphering the action mechanisms underlying the activities of bromelain observed in the present study to further confirm and enhance its application potential for therapeutic purposes.

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Bromelain Activity of Waste Parts of Two Pineapple Varieties

Cited by 8 publications

References 20 publications

Extraction, Partial Purification and Characterization of Bromelain from Pineapple (Ananas Comosus) Crown, Core and Peel Waste

Extraction, Partial Purification and Characterization of Bromelain from Pineapple (Ananas Comosus) Crown, Core and Peel Waste

Correction of Exocrine Pancreatic Insufficiency by Plant-Derived Enzymes (Literature Review)

Biochemical Characteristics and Healing Activity of Bromelia laciniosa Leaf Protease

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