Comparative stomach tissue distribution profiles of four major bio-active components of Radix Astragali in normal and gastric ulcer mice

Liu, Xiaohua; Xue, Zhixin; Wang, Bo; Wang, Ying; Zhang, Mengting; Feng, Shilan

doi:10.1590/s2175-97902022e18524

Cited by 1 publication

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References 12 publications

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“…Formononetin (F) and ononin (FG) are a pair (glycoside and aglycone) of isoflavones ( Scheme 1 ) and are the two major flavones found in various Chinese herbal medicines, such as astragali radix (the root of Astragalus membranaceus var. mongholicus or A. mem-branaceus ) [ 3 , 4 ] and red clover ( Trifolium pratense L.) [ 5 , 6 ]. F and FG have many pharmacological effects; for example, antineoplasm [ 6 , 7 , 8 , 9 ], antioxidation [ 10 ], anti-inflammation [ 11 , 12 ], neuroprotection [ 12 , 13 ], and blood-lipid regulation [ 14 , 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

A Study of the Mechanisms and Characteristics of Fluorescence Enhancement for the Detection of Formononetin and Ononin

Cao¹,

Li²,

Liu³

et al. 2023

Molecules

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In this work, the origins for the spectral difference between two isoflavones, formononetin (F) and ononin (FG), are revealed via a comparison study of the fluorescence molecular structure. The fluorescence enhancement of FG in hot alkaline conditions is reported for the first time. For F, there is almost no fluorescence under acidic conditions, but when the pH is >4.8, its fluorescence begins to increase due to the deprotonation of 7-OH. Under a pH between 9.3 and 12.0, the anionic form of F produces a strong and stable fluorescence. The fluorescence quantum yield (Yf) of F is measured to be 0.042. FG shows only weak fluorescence in aqueous solutions under a wide range of pH until it is placed in hot alkaline solutions, which is attributed to the cleavage reaction of the γ-pyrone ring in FG. The Yf of FG is determined to be 0.020. Based on the fluorescence sensitization methods of F and FG, the quantitative analysis and detection of two substances can be realized. The limit of the detections for F and FG are 2.60 ng·mL−1 and 9.30 ng·mL−1, respectively. The linear detection ranges of F and FG are 11.7~1860 ng·mL−1 and 14.6~2920 ng·mL−1, respectively. Although the structural relationship between F and FG is glycoside and aglycone, under hot alkaline conditions, the final products after the cleavage and hydrolysis reactions are essentially different. The different fluorescence characteristics between F and FG pave a way for further identification and a quantitative analysis of the corresponding components in Chinese herbal medicine.

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