Comparative sequence analysis of rotavirus genomic segment 6--the gene specifying viral subgroups 1 and 2

Both, Gerald W.; Siegman, L J; Bellamy, A R; Ikegami, Nobuko; Shatkin, Aaron J.; Furuichi, Yasuhiro

doi:10.1128/jvi.51.1.97-101.1984

Cited by 61 publications

(3 citation statements)

References 32 publications

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“…Gene segment 6 codes for VP6 (180). Depending on the strain of rotavirus, gene segment 8 or 9 codes for VP7 (25,180). For example, gene segment 8 codes for VP7 in the human serotype 2 rotavirus Hu/5 isolated in Melbourne, Australia (95), and for the bovine rotavirus UK (207).…”

Section: Molecular Biology and Classificationmentioning

confidence: 99%

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Human viral gastroenteritis.

Christensen¹

1989

Clinical Microbiology Reviews

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CHRISTENSEN propagated by using special cell types or techniques or both (320, 353, 372). In addition, a variety of in vitro detection systems, including enzyme immunoassays (149, 175, 333, 408), radioimmunoassays (137, 141, 181, 264), latex agglutination (140, 169), and nucleic acid hybridization techniques (116, 175), have been developed for their rapid detection. The detection and identification of these agents are important since viral gastroenteritis is the second most common clinical entity in developed countries, second only to viral upper respiratory tract illness (188). Worldwide, acute gastroenteritis and its associated dehydration afflicts almost 500 million children annually. In underdeveloped or developing countries, acute gastroenteritis, including viral gastroenteritis, is the leading cause of death of children under the age of 4 years (360). Since 1980, a wealth of literature has developed covering many aspects of these viruses and the infections they cause, including their molecular biology, epidemiology, immunology, and clinical features. In addition, detection tests for routine use have been developed for rotaviruses (310) and fecal adenoviruses (333) and experimental rotavirus vaccines have been developed (184, 377, 379, 380). Of these viruses, rotaviruses are the most common known cause of viral gastroenteritis in infants and young children, with the fastidious fecal adenoviruses most likely being the second most common cause in this age group (301, 369). Calici-, astro-, and coronaviruses are probably responsible for a minority of illness in the young age group (253, 302). In contrast, the Norwalk and Norwalk-like viruses have caused considerable numbers of outbreaks of gastroenteritis among older children, adolescents, and adults (136, 137, 189). ROTAVIRUSES Epidemiology Rotaviruses were first discovered in humans 15 years ago by Bishop et al. (18, 19) by the EM examination of duodenal

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Section: Molecular Biology and Classificationmentioning

confidence: 99%

“…VP6 is the major inner core structural protein which is present in large amounts in the virion. It has an apparent molecular weight of 42,000 (25,93,94,240,275). VP6 is the major subgroup antigen which can specify one of two rotavirus subgroups (subgroups I and II) (25,180).…”

Section: Molecular Biology and Classificationmentioning

confidence: 99%

Human viral gastroenteritis.

Christensen¹

1989

Clinical Microbiology Reviews

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“…1: Sedimentation coefficients, number of base pairs, and molecular weights (Mw) for rotavirus RNA segments and dsCARNA5. The numbers of base pairs of rotavirus RNA segments were determined by sequencing (segments 6-11 (21)(22)(23)(24)(25)(26)(27)) and by electron microscopy (segments 1-5 (28)). The S;O,values are extrapolated from Fig.…”

Section: Ultracentrifugation Concentration Of Isolated Segmentsmentioning

confidence: 99%

The stiffness of dsRNA: hydrodynamic studies on fluorescence-labelled RNA segments of bovine rotavirus

Kapahnke¹,

Rappold²,

Desselberger

et al. 1986

Nucl Acids Res

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The sedimentation coefficients of dsRNA segments of bovine rotavirus were determined in the analytical ultracentrifuge. The eleven segments were separated by preparative gel electrophoresis, and isolated by elution from gel pieces. The RNA was labelled by the intercalating fluorescent dye ethidium bromide at a ratio bound dye per base pair between 0.003 to 0.018. The analytical ultracentrifuge was equipped with a fluorescence recording optics. Sedimentation coefficients could be determined with amounts of RNA as little as 8 ng. All sedimentation coefficients were extrapolated to zero-concentration, zero-dye binding, and zero-impurities from the preparative gel electrophoresis. The hydrodynamic model of flexible cylinders was applied for the interpretation of the sedimentation coefficients. All dsRNA segments of rotavirus (663-3409 base pairs) and the dsRNA5 of cucumber mosaic virus (335 base pairs) fit the model of a "worm-like" or flexible cylinder with a persistence length of 1125 A and a hydrated diameter of 30 A. The results are compared with data from the literature on the persistence lengths of the B- and Z-forms of dsDNA and of viroids.

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Detection of a novel species A, DS‐1‐like, G4P[6] rotavirus strain from a Brazilian child with gastroenteritis

Bandeira

Souto

Souza

et al. 2021

Journal of Medical Virology

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Rotaviruses belonging to species A (RVA) remain among the most common causes of severe gastroenteritis in children aged <5 years, leading to substantial morbidity and mortality worldwide. Genome reassortment events between two human strains or human and animal strains represent one of the mechanisms which appear to generate the broad genetic variability of circulating. According to a nucleotide, sequence-based classification system, RVA strains are currently classified into three genotype constellations including Wa-like (genogroup I), DS-1-like (genogroup II), and AU-like (genogroup III). The present study reports the detection of an unusual RVA G4P[6] strain (coded as strain HSE005), which might have originated from a natural reassortment event between human and animal RVA strains. Molecular characterization of this isolate showed that it belonged to genogroup II, genotype G4P[6]. In addition, two genes (VP3 and NSP4) of this strain denoted evidence of reassortment events involving strains of distinct zoonotic evolutionary origins. Therefore, we propose that a new G4P[6] strain was identified, highlighting a possible first zoonotic transmission including a reassortment event that involved the VP3 gene.

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Comparative sequence analysis of rotavirus genomic segment 6--the gene specifying viral subgroups 1 and 2

Cited by 61 publications

References 32 publications

Human viral gastroenteritis.

Human viral gastroenteritis.

The stiffness of dsRNA: hydrodynamic studies on fluorescence-labelled RNA segments of bovine rotavirus

Detection of a novel species A, DS‐1‐like, G4P[6] rotavirus strain from a Brazilian child with gastroenteritis

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