Comparative Qualitative and Quantitative Assessment of Biomineralization of Tooth Development in Man and Zebrafish (<i>Danio rerio</i>)

Arnold, Wolfgang H.; Naumova, Kateryna I.; Naumova, Ella A.; Gaengler, Peter

doi:10.1002/ar.20687

Cited by 8 publications

(6 citation statements)

References 30 publications

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“…Normally, the zebrafish pharyngeal teeth are attached to the fifth branchial arch (also called the pharyngeal bone) and do not have tooth root encircled by periodontal tissues (Jackman & Stock, 2006). Nevertheless, the development and structure of the crown part of zebrafish's teeth are very similar to those of mammalian teeth (Arnold et al, 2008). The expression of zebrafish dlx3b and other dlx family genes was reported in 2006, and the dlx3b and dlx4b genes were found to be mainly expressed in the dental epithelium (Borday-Birraux et al, 2006;Verreijdt et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Mutantdlx3bdisturbs normal tooth mineralization and bone formation in zebrafish

Pang

Zhang

Shen

et al. 2020

PeerJ

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Background Tricho-dento-osseous (TDO) syndrome is an autosomal dominant disorder characterized by anomalies in hair, teeth and bone (OMIM190320). Various mutations of Distal-Less 3 (DLX3) gene are found to be responsible for human TDO. The aim of this study was to investigate effects of DLX3 on tooth and bone development using a zebrafish model. Methods The dlx3b mutant zebrafish lines were established using the gene targeting tool transcription activator-like effector nuclease (TALEN). Micro-computed tomography was used to render the three-dimensional skeletal structures of mutant fishes. The pharyngeal bone along with connected teeth was isolated and stained by Alizarine Red S, then observed under stereomicroscope. Scanning electron microscopy (SEM) and energy dispersive spectrometer (EDS) were used to examine the tooth surface morphology and mineral composition. Quantitative real-time PCR was used to analyze gene expression. Results A moderate curvature of the spine toward the dorsal side was found at the early larval stages, appearing in 86 out of 100 larvae in dlx3b-/- group as compared to 3 out of 99 in the dlx3b+/+ group. At the adult stage, three of the thirty dlx3b-/- homozygotes exhibited prominent abnormal curvature in the spine. SEM revealed morphological surface changes in pharyngeal teeth enameloid, accompanied by a decrease in the mineral content detected by EDS. Furthermore, specific secretory calcium-binding phosphoprotein (SCPP) genes, including odam, scpp9, spp1, scpp1, and scpp5 were significantly downregulated in dlx3b mutants. Conclusion The findings of this study suggest that dlx3b is critical for enamel mineralization and bone formation in zebrafish. Moreover, the discovery of the downregulation of SCPP genes in dlx3b mutants sheds new light on the molecular mechanisms underlying TDO syndrome.

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Section: Discussionmentioning

confidence: 99%

Mutantdlx3bdisturbs normal tooth mineralization and bone formation in zebrafish

Pang

Zhang

Shen

et al. 2020

PeerJ

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“…Computer-assisted histomorphometry is dependent upon sectioning and staining methods (Dai et al, 2005;Plenk, 2010;Sherif et al, 2006Sherif et al, , 2009 and is important for constructing morphological equivalents of the tissue structures in two-dimensions. X-ray microanalysis of unstained slices of teeth (Arnold and Gaengler, 2007; Arnold et al, 2008), immunohistochemistry (Erben, 1997;Rammelt et al, 2007;Rippstein et al, 2006;Wolf et al, 1992;Yang et al, 2003), immunofluorescence (Rippstein et al, 2006;Singhrao et al, 2009, in situ hybridization (ISH) (Saito et al, 1998;Singhrao and Nair-Roberts, 2010), and fluorescent Tunel assays (Singhrao et al, unpublished data) are all possible using this technique.…”

Section: Which Allied Histology Applicationsmentioning

confidence: 97%

“…However, as demand for the technique grows, the technology in the field is also expanding and this is demonstrated by the saw microtome (Leica SP1600) that can also cut sections of tissue-metal composites from 80 lm thicknesses. This method is advocated for use with teeth for X-ray microanalysis to map the elements of mineralized tooth structures (Arnold and Gaengler, 2007;Arnold et al, 1998Arnold et al, , 2008. Other methods include sectioning by using a high speed, isomet saw, followed by grinding using the Ecomet 2 or the Automet 2 grinders (Buehler Krautkramer, Coventry, UK) to the required thickness.…”

Section: Sectioningmentioning

confidence: 99%

Informed choices for challenging specimens when choosing methacrylate resin systems for histology

Singhrao

Nicholson

Crean

2011

Microscopy Res & Technique

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Functional restoration for orthopedic, systemic, or dental purposes uses a diverse range of biomaterials. Monitoring for any subsequent failures associated with pathology is vital. To this end, an alternative methodology beyond that of cutting sections by conventional microtome knife-based histomethodologies was pioneered. The method was to saw a block of tissue followed by grinding the unsupported slice to the required thickness. The sawing and grinding of unsupported bones or teeth exposed not only the specimen fragility, but also the delicacy of the associated soft tissues. Although incomplete, the significance of the technique was recognized and improvements were incorporated which persist to the present day addressing the histopathology of challenging specimens. One major improvement for preserving cellular structure as well as reducing fracture incidence was achieved by impregnating tissue samples with cross-linked resins prior to the cutting and grinding processes. Methyl and glycol methacrylate resins superseded all other choices because of the advantages of photo-initiated curing. Further pioneering work led to the formulation of increasingly appropriate resin systems and the subsequent processing protocols evolved, as clinical needs for histology-specific techniques became apparent. Over the decades, several key pioneers entered this scientific arena to develop the now established resin formulation, processing protocols, and allied applications. This has led to the commercialization of the various resin systems. The aim here is to discuss the commercially available glycol and methyl methacrylate resin systems and their practical application to a variety of important techniques used in the histological assessment of complex pathology specimens.

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“…[19][20][21][22][23] Surveying the prior studies, the scanning electron microscope was found to be well suited for the analysis of the developmental defects of enamel surface. It is a type of electron microscope that images the sample surface by scanning it with a high-energy beam of electrons in a raster scan pattern.…”

Section: Sem-edx Analysis For Surface Aberrations Of Neonate's Teeth mentioning

confidence: 99%

SEM-EDX Analysis for Surface Aberrations of Neonate's Teeth Influenced by the use of Lithium in Pregnancy

Nazir¹,

Naqvi²,

Masood³

et al. 2011

World Journal of Dentistry

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Objective of the study: Developmental defects of enamel may lead to esthetic tribulations. Malformed dental enamel is usually susceptible to caries and sensitivity of teeth. The objective of the present study was to evaluate the distribution of developmental defects affecting the enamel surfaces in the teeth of newborn babies of the mothers, caused by the use of lithium for a long time during pregnancy. Study design/methods:The female rabbits were kept on this drug during pregnancy and their offsprings were used for the study. They were sacrificed to obtain their jaws. Teeth were extracted for assessment of the congenital defects developed during intrauterine life. Surface structure was studied by scanning electron microscopy and energy-dispersive X-ray microanalysis (SEM-EDX). Results:The results showed that the drug had caused erosions of the incisors and the molars, as compared to the premolars. Conclusion:According to the results, incisors and the molars appeared esthetically and functionally compromised teeth. The drug should, therefore, be used by doctor's prescription only, especially during pregnancy, avoiding the teratogenic effect on the dentition of the newborns.

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Comparative Qualitative and Quantitative Assessment of Biomineralization of Tooth Development in Man and Zebrafish (Danio rerio)

Cited by 8 publications

References 30 publications

Mutantdlx3bdisturbs normal tooth mineralization and bone formation in zebrafish

Mutantdlx3bdisturbs normal tooth mineralization and bone formation in zebrafish

Informed choices for challenging specimens when choosing methacrylate resin systems for histology

SEM-EDX Analysis for Surface Aberrations of Neonate's Teeth Influenced by the use of Lithium in Pregnancy

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