Comparative proteomics of copper exposure and toxicity in rainbow trout, common carp and gibel carp

Eyckmans, Marleen; Benoot, D.; Raemdonck, Geert A.A. Van; Zegels, Geert; Ostade, Xaveer Van; Witters, Erwin; Blust, Ronny; Boeck, Gudrun De

doi:10.1016/j.cbd.2012.03.001

Cited by 27 publications

(15 citation statements)

References 87 publications

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“…Before the experiments were started, fish were netted and anesthetized (50 mg/L tricaine methanesulfonate buffered with 125 mg/L NaHCO 3 ) until equilibrium was lost, and PIT-tagged with BioMark, 8.4-mm, 134.2-kHz tags (HPT8, BioMark, Boise, Idaho) using a 6-gauge trocar injection needle. Fish were treated prophylactically with oxolinic acid (2 mg/g food) for 10 d to treat for the possibility of bacterial infection (Austin et al 1983) and with formalin immersion (1:10,000, 1:8,000, and then 1:6,000 for 1.5 h each) to treat for the possibility of infection with external parasites to alleviate any hsp 70 stress response that could be induced by disease Iwama et al 1999;Eyckmans et al 2012); hsp 70 does not appear to be affected by formalin (Zarate and Bradley 2003).…”

Section: Methodsmentioning

confidence: 99%

The Effects of Semichronic Thermal Stress on Physiological Indicators in Steelhead

Kammerer

Heppell

2013

Trans Am Fish Soc

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The physiological response of juvenile steelhead Oncorhynchus mykiss to prolonged heat stress was examined by exposing replicated groups of fish to three different temperatures, 15, 23, and 25 • C, for 25 consecutive days followed by a 55-d recovery period at 15 • C. We found that at temperatures ≥ 25 • C, steelhead consumed significantly less food per day and had elevated feed conversion rates but experienced slower growth, reduced body size, lower body fat, and elevated heat shock protein (hsp) 70 levels relative to fish exposed to 23 • C and 15 • C. Growth decreased 24.4% and 27.1% for length and mass, respectively, between 15 • C and 23 • C, and an additional 60% and 56.5%, respectively, between 23 • C and 25 • C during exposures. While growth increments and lipid levels recovered to control levels after water temperature was reduced, body size of the fish exposed to 25 • C lagged throughout the experiment. Our results indicate a temperature threshold after which steelhead exposed to semichronic thermal stress incur a physiological debt. Heat shock protein 70 levels were detectable up to 25 d poststress in fin and liver tissues. This result provides evidence that hsp 70 is a useful metric for measuring thermal stress that can be assessed nonlethally and used to monitor thermal-habitat restoration efforts for threatened and endangered salmonids.

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Section: Methodsmentioning

confidence: 99%

The Effects of Semichronic Thermal Stress on Physiological Indicators in Steelhead

Kammerer

Heppell

2013

Trans Am Fish Soc

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“…A more direct correlation is expected for proteins and biological processes [16], making quantitative mass spectrometric (MS) proteomics an attractive approach [17], and a relative quantification approach based on MS results is the ultimate way to go. Moreover, the isobaric tags for relative and absolute quantitation (iTRAQ) are demonstrated to be more reliable and accurate than two-dimensional differential gel electrophoresis and other proteomic methods in identifying and quantifying proteins [18], and have been increasingly applied to examine differentially expressed proteins in different aquatic animals, such as zebrafish (Danio rerio), marine medaka (Oryzias melastigma) and Chinese mitten crab Eriocheir sinensis, in response to different pathogens, i.e. Aeromonas hydrophila, 2,2′,4,4′-tetrabromodiphenyl ether (BDE-47) and Spiroplasma eriocheiris, in order to identify the pathogen responsive proteins [19][20][21].…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic and proteomic analyses of splenic immune mechanisms of rainbow trout (Oncorhynchus mykiss) infected by Aeromonas salmonicida subsp. salmonicida

Long

Zhao

et al. 2015

Journal of Proteomics

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“…The tank water was changed weekly. Ammonia, nitrate and nitrite levels were kept below toxic concentrations (<0.1 mg/L) (Eyckmans et al, 2012).…”

Section: Daphnia Magna and Limnodrilus Hoffmeisterimentioning

confidence: 99%

Ecotoxicological assessment of flocculant modified soil for lake restoration using an integrated biotic toxicity index

2016

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a b s t r a c tFlocculant modified soils/clays are being increasingly studied as geo-engineering materials for lake restoration and harmful algal bloom control. However, the potential impacts of adding these materials in aquatic ecological systems remain unclear. This study investigated the potential effects of chitosan, cationic starch, chitosan modified soils (MS-C) and cationic starch modified soils (MS-S) on the aquatic organisms by using a bioassay battery. The toxicity potential of these four flocculants was quantitatively assessed using an integrated biotic toxicity index (BTI). The test system includes four aquatic species, namely Chlorella vulgaris, Daphnia magna, Cyprinus carpio and Limnodrilus hoffmeisteri, which represent four trophic levels in the freshwater ecosystem. Results showed that median effect concentrations (EC 50 ) of the MS-C and MS-S were 31e124 times higher than chitosan and cationic starch, respectively. D. magna was the most sensitive species to the four flocculants. Histological examination of C. carpio showed that significant pathological changes were found in gills. Different from chitosan and cationic starch, MS-C and MS-S significantly alleviated the acute toxicities of chitosan and cationic starch. The toxicity order of the four flocculants based on BTI were cationic starch > chitosan > MS-S > MS-C. The results suggested that BTI can be used as a quantitative and comparable indicator to assess biotic toxicity for aquatic geo-engineering materials. Chitosan or cationic starch modified soil/clay materials can be used at their optimal dosage without causing substantial adverse effects to the bioassay battery in aquatic ecosystem.

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Comparative proteomics of copper exposure and toxicity in rainbow trout, common carp and gibel carp

Cited by 27 publications

References 87 publications

The Effects of Semichronic Thermal Stress on Physiological Indicators in Steelhead

The Effects of Semichronic Thermal Stress on Physiological Indicators in Steelhead

Transcriptomic and proteomic analyses of splenic immune mechanisms of rainbow trout (Oncorhynchus mykiss) infected by Aeromonas salmonicida subsp. salmonicida

Ecotoxicological assessment of flocculant modified soil for lake restoration using an integrated biotic toxicity index

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