Comparative proteomics, network analysis and post-translational modification identification reveal differential profiles of plasma Con A-bound glycoprotein biomarkers in gastric cancer

Uen, Yih-Huei; Lin, Kai; Sun, Ding; Liao, Chen Chung; Hsieh, Ming‐Hong; Huang, Yung Kai; Chen, Yen Wei; Huang, Pei Hsuan; Chen, Wei Jung; Tai, Chih Chun; Lee, Kuan Wei; Chen, You Chia; Lin, Ching Yu

doi:10.1016/j.jprot.2013.03.007

Cited by 60 publications

(55 citation statements)

References 101 publications

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“…Levels of LRG1 expression were negatively correlated with FIGO (International Federation of Gynecology and Obstetrics) stage and lymphatic metastasis [19]. Besides, LRG1 expressions were different in certain kinds of tumors and many benign diseases from healthy subjects [12][13][14][15][16][17][18]. In this study, we first uncovered that the levels of LRG1 mRNA and protein were significantly downregulated in HCC tissues compared with their corresponding peritumoral tissues (Fig.…”

Section: Discussionmentioning

confidence: 76%

“…It could be served as a diagnostic or prognostic biomarker for certain inflammatory conditions in hepatocytes like CRP [14] and neurodegeneration in mouse cerebral cortex [15]. Recently, accumulating evidences showed that LRG1 was closely correlated with various kinds of tumors such as ovarian cancer [16], gastric cancer [17], and non-small cell lung cancer [18]. Moreover, LRG1 was an independent prognostic factor for overall survival of endometrial carcinoma patients and offered clinical value in directing personal treatment for endometrial carcinoma patients [19].…”

Section: Introductionmentioning

confidence: 99%

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LRG1 suppresses the migration and invasion of hepatocellular carcinoma cells

et al. 2015

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Hepatocellular carcinoma (HCC) is a malignant tumor driven by complex pathological mechanisms and is characterized by fast progression and poor prognosis. The main cause of death in HCC patients is tumor metastasis. However, underlying molecular mechanisms of metastasis are largely unknown in HCC. In the present study, a novel metastasis-related gene, leucine-rich-alpha-2-glycoprotein 1 (LRG1), was identified in HCC. We revealed that LRG1 expression was downregulated in HCC tissues by quantitative real-time PCR and immunohistochemical staining. In vitro assays demonstrated LRG1 had no effect on cell proliferation. Migratory and invasive potential of HCC cells was reduced by ectopic overexpression of LRG1, whereas silencing LRG1 could enhance migration and invasion of HCC cells. Furthermore, exogenous recombinant human protein of LRG1 could inhibit migration and invasion of HCC cells in vitro. The above findings indicate that LGR1 is involved in the inhibition of HCC metastasis and it may function as a novel metastasis suppressor in HCC.

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Section: Discussionmentioning

confidence: 76%

Section: Introductionmentioning

confidence: 99%

LRG1 suppresses the migration and invasion of hepatocellular carcinoma cells

et al. 2015

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“…[116][117][118][119] The majority of plasma proteins are glycosylated, and several studies have analyzed glycoproteins or enzymatically released glycans in plasma or serum of gastric cancer patients and controls. 120,121 Although the study of protein glycosylation is a newly emerging field for biomarker discovery, it has potential application for diagnosis and prognosis of gastric cancer. For example, preliminary studies suggest that non-sulfated chondroitin/dermatan sulfate concentration is increased in gastric biopsies from cancer patients compared with normal controls.…”

Section: Discovery Of Novel Biomarkersmentioning

confidence: 99%

Biomarkers ofHelicobacter pylori-associated gastric cancer

2013

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“…We purified the S-nitrosoproteins by a biotin-switch method, labeled them with the iTRAQ reagent, and identified them by tandem mass spectrometry. Ingenuity Pathway Analysis (IPA) software can predict the network and interplay of proteins, in particular within signaling pathways to allow further investigation into the roles of these proteins in the indicated physiological processes [16]. In the present study, the identified S-nitrosoproteome modulated by CPT was analyzed by IPA.…”

Section: Introductionmentioning

confidence: 99%