Biomarkers of<i><i>Helicobacter pylori</i>-</i>associated gastric cancer

Cooke, Cara L.; Torres, Javier; Solnick, Jay V.

doi:10.4161/gmic.25720

Cited by 20 publications

(21 citation statements)

References 138 publications

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“…Nevertheless, if the fingerprint of a cancer promoting microbial community could be identified, this might provide another biomarker that could be used to identify H. pylori-infected patients who are at greatest risk of gastric cancer. 45 …”

Section: Does the Gastric Microbiota Change H Pylori Colonization Ormentioning

confidence: 99%

The gastric microbial community,Helicobacter pyloricolonization, and disease

Martín

Solnick

2014

Gut Microbes

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Section: Does the Gastric Microbiota Change H Pylori Colonization Ormentioning

confidence: 99%

The gastric microbial community,Helicobacter pyloricolonization, and disease

Martín

Solnick

2014

Gut Microbes

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“…However, endoscopy is an invasive and a costly method that requires special equipment and well-trained personnel to offer reliable diagnosis. Despite all recent developments in molecular diagnostics (16), identification of biomarkers with reliable predictive value is still unavailable. Peripheral blood mtDNA copy number has recently emerged as a potential preventive/diagnosis biomarker associated with the risk of various cancers (18,19).…”

Section: Discussionmentioning

confidence: 99%

“…In the case of intestinal-type gastric cancer, the measure of pepsinogen levels has been shown to be useful to detect gastric atrophy, although its use in non-Asian countries is controversial (15). Thus, despite several efforts to develop biomarkers to identify patients at risk for distal gastric cancer (16), no efficacious screening test is yet available.…”

Section: Introductionmentioning

confidence: 99%

Circulating Mitochondrial DNA Level, a Noninvasive Biomarker for the Early Detection of Gastric Cancer

Fernandes

Michel

Camorlinga‐Ponce³

et al. 2014

Cancer Epidemiology, Biomarkers &Amp; Prevention

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Background: Gastric cancer represents a major health burden worldwide and is often diagnosed at an advanced stage. Biomarkers for screening and prevention of gastric cancer are missing. Changes in peripheral blood mitochondrial DNA (mtDNA) have emerged as a potential preventive/diagnosis biomarker for cancer risk. We aimed to determine whether peripheral leukocytes mtDNA levels are associated with stages of the gastric carcinogenesis cascade.Methods: We measured mtDNA by quantitative real-time PCR assay in peripheral leukocytes of 28 patients with non-atrophic gastritis (NAG), 74 patients with gastric cancer, and 48 matched asymptomatic controls. In parallel, the serologic level of IL8 was determined.Results: Mean mtDNA level was higher in patients with gastric cancer (P ¼ 0.0095) than in controls, with values >8.46 significantly associated with gastric cancer (OR, 3.93). Three ranges of mtDNA values were identified: interval I, <2.0; interval II, 2.0-20; and interval III, >20. Interval I included mainly NAG cases, and few gastric cancer samples and interval III corresponded almost exclusively to patients with gastric cancer. All controls fell in interval II, together with some NAG and gastric cancer cases. IL8 levels were significantly higher in patients with gastric cancer (P < 0.05), with levels >50 pg/mL observed exclusively in patients with gastric cancer, allowing to distinguish them within interval II. We validated mtDNA results in a second cohort of patients, confirming that mtDNA was significantly higher in gastric cancer than in patients with preneoplasia.Conclusions: Circulating levels of mtDNA and IL8 constitute a potential biomarker for the early detection of gastric cancer.Impact: Our findings lead us to propose a new noninvasive method to detect patients with gastric cancer risk. Cancer Epidemiol Biomarkers Prev; 23(11); 2430-8. Ó2014 AACR.

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“…This Gram-negative species chronically infects some half of all people worldwide, disproportionately in developing countries, typically for decades, and is a major cause of peptic ulcer disease and gastric cancer. [58][59][60] H. pylori's TRANSPOSABLE ELEMENTS It was difficult to completely ignore mobile DNA elements, to forgo the excitement of serendipitous discovery and preliminary characterization of new elements, after nominally shifting my research focus to H. pylori. In particular, in analyzing genomes of diverse H. pylori strains from various human populations, Dange Kersulyte and I were intrigued to discover two novel IS families: one, represented by the elements IS605, IS606, ISHp608 (or IS608) and ISHp609 (or IS609), whose movement is driven by a very small, novel tyrosine-type transposase; [61][62][63] and second, IS607, that encodes a novel serine-type transposase.…”

Section: Tn5 Transposition Mechanismmentioning

confidence: 99%

Julian Davies and the discovery of kanamycin resistance transposon Tn5

Berg

2016

J Antibiot

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This paper recounts some of my fond memories of a collaboration between Julian Davies and myself that started in 1974 in Geneva and that led to our serendipitous discovery of the bacterial kanamycin resistance transposon Tn5, and aspects of the lasting positive impact of our interaction and discovery on me and the community. Tn5 was one of the first antibiotic resistance transposons to be found. Its analysis over the ensuing decades provided valuable insights into mechanisms and control of transposition, and led to its use as a much-valued tool in diverse areas of molecular genetics, as also will be discussed here. The Journal of Antibiotics (2017) 70, 339-346; doi:10.1038/ja.2016.120; published online 12 October 2016 BACKGROUND It was wonderfully educational, productive and exciting for me to work with Julian Davies for a few years in the mid 1970s, doing experiments that led to the serendipitous discovery and initial characterizations of kanamycin resistance transposon Tn5 1 -one of the first and most useful of the many bacterial antibiotic resistance (AR) transposons that have been found. 2 I was inspired by Julian's knowledge of mechanisms of antibiotic action and resistance mechanisms, his creativity and clarity of thought and expression, as illustrated in his numerous papers, 3-6 and by his high energy, kindness and inventive good humor-traits that have enriched the lives and careers of many people over the years. Described here, in tribute to Julian and his special character, are my memories of a collaboration that led to Tn5's unexpected discovery, Tn5's properties, and some lasting impacts of our collaboration on the field and on me personally.We began interacting in 1974, soon after Julian arrived in Geneva to begin his sabbatical with Pierre Francois Spahr (Department of Molecular Biology of the University). 7 I was then in my fourth (final) year as Chargé de Recherche (senior postdoc) in Lucien Caro's group in the same department, studying λdv plasmids, often in collaboration with Grete Kellenberger-Gujer, also in Lucien's group 8-13 -and during these years, also hugely enjoying features in and out of the lab that have drawn researchers to Geneva for decades: a superb scientific atmosphere, year-round easy access to the exquisite Alps and Jura mountains, and the language, cuisine and culture of Geneva and vicinity. I had become intrigued the year before by Julian's ideas about origins and evolution of resistance genes, as described in his paper with Raoul Benveniste. 14 They advanced the then-novel theory that the types of bacteria that had provided life saving antibiotics were also the ancestral sources of AR genes that were becoming increasingly common in human and veterinary pathogens. Their inferences were

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Biomarkers ofHelicobacter pylori-associated gastric cancer

Cited by 20 publications

References 138 publications

The gastric microbial community,Helicobacter pyloricolonization, and disease

The gastric microbial community,Helicobacter pyloricolonization, and disease

Circulating Mitochondrial DNA Level, a Noninvasive Biomarker for the Early Detection of Gastric Cancer

Julian Davies and the discovery of kanamycin resistance transposon Tn5

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