Comparative proteomics and gene expression analysis in Arachis duranensis reveal stress response proteins associated to drought tolerance

Carmo, Lílian S.T.; Martins, Andressa Cunha Quintana; Martins, C.; Passos, Mário A.S.; Silva, Luciano; Araújo, Ana Cláudia Guerra; Brasileiro, Ana Cristina Miranda; Miller, Robert G.; Guimarães, P. M.; Mehta, Ângela

doi:10.1016/j.jprot.2018.09.011

Cited by 24 publications

(17 citation statements)

References 57 publications

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“…Wild and cultivated Arachis genotypes exhibit contrasting transpiration behaviors under water-limited conditions, with variable levels of water stress tolerance among the wild species 43 . Accordingly, the accessions K7988 of A. duranensis and V10309 of A. stenosperma have been selected as the drought-tolerant and the drought-sensitive genotypes, respectively, in our functional genomics studies 18 , 20 , 21 , 23 , 24 . Here, the comprehensive expression analysis conducted in these genotypes revealed that most of the 28 A. duranensis genes involved in RFO metabolism, and their orthologs in A. stenosperma , are responsive to drought imposition.…”

Section: Discussionmentioning

confidence: 99%

“…This data comprises: (1) Transcripts expressed in roots of A. duranensis and A. stenosperma plants submitted to dehydration treatment, by the withdrawal of hydroponic nutrient solution from 25 to 150 min 21 and pooled in equal amounts, a treatment which we previously showed to induce major alterations in the transcriptome of these species. (2) Transcripts expressed in A. duranensis and A. stenosperma plants subjected to a decreased in soil availability with withholding of irrigation for four days, a treatment which induced proteomic and transcriptomic alterations 24 . The differential expression values (log2 of fold-change) between stressed and control samples in A. duranensis and A. stenosperma roots were plotted in a heatmap graph using the heatmap2 from ggplot R package, as previously described 23 .…”

Section: Methodsmentioning

confidence: 99%

“…Over the past years, we have exploited the peanut wild relatives gene pool as a source of valuable traits for peanut breeding, such as resistance/tolerance to abiotic and biotic stresses 18 . Progress in functional genomics of wild Arachis led to the discovery of many genes and proteins involved in water deficit responses in the drought-tolerant A. duranensis and in the drought-sensitive A. stenosperma , including genes involved in RFO metabolism 19 – 24 . Furthermore, the availability of the complete genome sequences of the wild ancestral species of peanut, A. duranensis and A. ipaënsis ( https://www.peanutbase.org/ ), has enabled genome-wide analysis and contributed to new insights into the molecular function and evolution of gene families in the genus Arachis 25 .…”

Section: Introductionmentioning

confidence: 99%

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Characterization of raffinose metabolism genes uncovers a wild Arachis galactinol synthase conferring tolerance to abiotic stresses

Vinson

Mota

Porto

et al. 2020

Sci Rep

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Raffinose family oligosaccharides (RFOs) are implicated in plant regulatory mechanisms of abiotic stresses tolerance and, despite their antinutritional proprieties in grain legumes, little information is available about the enzymes involved in RFO metabolism in Fabaceae species. In the present study, the systematic survey of legume proteins belonging to five key enzymes involved in the metabolism of RFOs (galactinol synthase, raffinose synthase, stachyose synthase, alpha-galactosidase, and beta-fructofuranosidase) identified 28 coding-genes in Arachis duranensis and 31 in A. ipaënsis. Their phylogenetic relationships, gene structures, protein domains, and chromosome distribution patterns were also determined. Based on the expression profiling of these genes under water deficit treatments, a galactinol synthase candidate gene (AdGolS3) was identified in A. duranensis. Transgenic Arabidopsis plants overexpressing AdGolS3 exhibited increased levels of raffinose and reduced stress symptoms under drought, osmotic, and salt stresses. Metabolite and expression profiling suggested that AdGolS3 overexpression was associated with fewer metabolic perturbations under drought stress, together with better protection against oxidative damage. Overall, this study enabled the identification of a promising GolS candidate gene for metabolic engineering of sugars to improve abiotic stress tolerance in crops, whilst also contributing to the understanding of RFO metabolism in legume species.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Characterization of raffinose metabolism genes uncovers a wild Arachis galactinol synthase conferring tolerance to abiotic stresses

Vinson

Mota

Porto

et al. 2020

Sci Rep

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“…Moreover, heat shock 70 kDa protein (Csa_4G617390) was detected in the transcriptomic data during H 2 S regulation of saltstress tolerance in cucumber (Table S2). Heat shock proteins are also associated with the stress response and play an important role as chaperones, including in protein folding and degradation under stress conditions (Carmo et al, 2019).…”

Section: Defense Related Pathwaysmentioning

confidence: 99%

H2S Regulation of Metabolism in Cucumber in Response to Salt-Stress Through Transcriptome and Proteome Analysis

Jiang

Ren

et al. 2020

Front. Plant Sci.

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In a previous study, we found that H 2 S alleviates salinity stress in cucumber by maintaining the Na + /K + balance and by regulating H 2 S metabolism and the oxidative stress response. However, little is known about the molecular mechanisms behind H 2 S-regulated saltstress tolerance in cucumber. Here, an integrated transcriptomic and proteomic analysis based on RNA-seq and 2-DE was used to investigate the global mechanism underlying H 2 S-regulated salt-stress tolerance. In total, 11,761 differentially expressed genes (DEGs) and 61 differentially expressed proteins (DEPs) were identified. Analysis of the pathways associated with the DEGs showed that salt stress enriched expression of genes in primary and energy metabolism, such as photosynthesis, carbon metabolism and biosynthesis of amino acids. Application of H 2 S significantly decreased these DEGs but enriched DEGs related to plant-pathogen interaction, sulfur-containing metabolism, cell defense, and signal transduction pathways. Notably, changes related to sulfur-containing metabolism and cell defense were also observed through proteome analysis, such as Cysteine synthase 1, Glutathione S-transferase U25-like, Protein disulfide-isomerase, and Peroxidase 2. We present the first global analysis of the mechanism underlying H 2 S regulation of salt-stress tolerance in cucumber through tracking changes in the expression of specific proteins and genes.

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“…While RCA is involved in the activation of Rubisco via ATP dependent carboxylation, RUBISCO participates in carbon fixation (Salvucci and Ogren, 1996). The homolog of GLYCINE-RICH PROTEIN DOT1-like (DOT1) is known for its role in vascular tissue pattern formation and maintenance of cellular structure for adaptation under drought (Carmo et al, 2019). It was abundant in both G1 and G3 probably because sucrose at high concentrations is known to induce osmotic stress and drought like conditions.…”

Section: Discussionmentioning

confidence: 99%

Proteome Analysis of the Gametophytes of a Western Himalayan Fern Diplazium maximum Reveals Their Adaptive Responses to Changes in Their Micro-Environment

et al. 2019

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Ferns have survived changing habitats and environmental extremes of different eras, wherein, the exploratory haploid gametophytes are believed to have played a major role. Therefore, the proteome of in vitro grown gametophytes of a temperate Himalayan fern, Diplazium maximum in response to 0 (G0), 1 (G1), and 3% (G3) sucrose was studied. A total of 110 differentially abundant protein spots (DAPs) were obtained. Among these, only 67 could be functionally categorized as unique proteins involved in various metabolic processes. Calcium dependent proteins, receptor like kinases, G proteins, proteins related to hormonal signaling and their interaction with other pathways, and regulatory proteins were recorded indicating the involvement of five different signaling pathways. DAPs involved in the activation of genes and transcription factors of signaling and transduction pathways, transport and ion channels, cell-wall and structural proteins, defense, chaperons, energy metabolism, protein synthesis, modification, and turnover were identified. The gametophytes responded to changes in their micro-environment. There was also significant increase in prothallus biomass and conversion of two-dimensional prothalli into three-dimensional prothallus clumps at 3% sucrose. The three-D clumps had higher photosynthetic surface area and also closer proximity for sexual reproduction and sporophyte formation. Highest accumulation of proline, enhanced scavenging of reactive oxygen species (ROS) and DAPs of mostly, abiotic stress tolerance, secondary metabolite synthesis, and detoxification at 3% sucrose indicated an adaptive response of gametophytes. Protein Protein Interaction network and Principal Component analyses, and qRT-PCR validation of genes encoding 12 proteins of various metabolic processes indicated differential adjustment of gametophytes to different levels of sucrose in the culture medium. Therefore, a hypothetical mechanism was proposed to show that even slight changes in the micro-environment of D. maximum gametophytes triggered multiple mechanisms of adaptation. Many DAPs identified in the study have potential use in crop improvement and metabolic engineering programs, phytoremediation and environmental protection.

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Comparative proteomics and gene expression analysis in Arachis duranensis reveal stress response proteins associated to drought tolerance

Cited by 24 publications

References 57 publications

Characterization of raffinose metabolism genes uncovers a wild Arachis galactinol synthase conferring tolerance to abiotic stresses

Characterization of raffinose metabolism genes uncovers a wild Arachis galactinol synthase conferring tolerance to abiotic stresses

H2S Regulation of Metabolism in Cucumber in Response to Salt-Stress Through Transcriptome and Proteome Analysis

Proteome Analysis of the Gametophytes of a Western Himalayan Fern Diplazium maximum Reveals Their Adaptive Responses to Changes in Their Micro-Environment

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