Comparative proteomics analysis reveals the molecular mechanism of enhanced cold tolerance through ROS scavenging in winter rapeseed (Brassica napus L.)

Abstract

Two winter rapeseed cultivars, “NS” (cold tolerant) and “NF” (cold sensitive), were used to reveal the morphological, physiological, and proteomic characteristics in leaves of plants after treatment at -4°C for 12 h(T1) and 24 h(T2), and at room temperature(T0), to understand the molecular mechanisms of cold tolerance. Antioxidant activity and osmotic adjustment ability were higher, and plasma membrane injury was less obvious, in NS than in NF under cold stress. We detected different abundant proteins (DAPs) related to cold tolerance in winter rapeseed through data-independent acquisition (DIA). Compared with NF, A total of 1,235 and 1,543 DAPs were identified in the NSs under T1 and T2, respectively. Compared with NF, 911 proteins were more abundant in NS only after cold treatment. Some of these proteins were related to ROS scavenging through four metabolic pathways: lysine degradation; phenylalanine, tyrosine, and tryptophan; flavonoid biosynthesis; and ubiquinone and other terpenoid-quinone biosynthesis. Analysis of these proteins in the four candidate pathways revealed that they were rapidly accumulated to quickly enhance ROS scavenging and improve the cold tolerance of NS. These proteins were noticeably more abundant during the early stage of cold stress, which was critical for avoiding ROS damage.

Comparative Transcriptomics and Proteomics Analyses of Leaves Reveals a Freezing Stress-Responsive Molecular Network in Winter Rapeseed (Brassica rapa L.)

“…The two extension winter rapeseed cultivars in Northwest China, freezing-resistant cultivar 17NTS57 (NS, with more than 85% overwinter survival rate at −26°C, dark brown seed, 33.02% oleic acid, and 17.29% linoleic acid) and freezing-sensitive cultivar NQF24 (NF, with 0% overwinter survival rate below −10°C, dark brown seed, 36.98% oleic acid, and 16.73% linoleic acid), used in this study were provided by Gansu Agricultural University ( Mi et al, 2021 ). The seedlings of the two cultivars were grown in plastic pots (5 L) filled with soil and normally maintained.…”

Role of Reactive Oxygen Species and Hormones in Plant Responses to Temperature Changes

“…Furthermore, the gene expression in response to cold stress may be regulated by redox signaling, as several defense genes containing antioxidant-responsive elements in their promoter regions [88]. More recently, the proteomics analysis in Brassica napus L. showed that the molecular mechanism of enhanced cold tolerance was achieved through ROS scavenging via the metabolic pathways [166].…”

Integrated methylome and transcriptome analysis unravel the cold tolerance mechanism in winter rapeseed(Brassica napus L.)

“…Over the past decade, our research group has successfully selected and bred rapeseed cultivars of B. napus that can overwinter safely in most of the northwestern areas from the 38° to the 42° north latitude, where the lowest temperature in winter is -26℃ [15,16]. Moreover, the cold tolerance characteristics of these rapeseed cultivars at the transcription, protein, physiological, biochemical, and subcellular levels were studied [15,21,22]. The strong coldresistant cultivar had stronger antioxidant enzyme activity and accumulation of cryoprotective molecules, associated with the metabolic pathways, including alpha-linolenic acid metabolism, plant hormone signal transduction, microbial metabolism in diverse environments, linoleic acid metabolism, phenylpropanoid biosynthesis, glutathione metabolism, flavonoid biosynthesis metabolism, and amino acid metabolism.…”