Comparative Proteomic Analysis of Soybean Leaves and Roots by iTRAQ Provides Insights into Response Mechanisms to Short-Term Salt Stress

Abstract

Salinity severely threatens land use capability and crop yields worldwide. Understanding the mechanisms that protect soybeans from salt stress will help in the development of salt-stress tolerant leguminous plants. Here we initially analyzed the changes in malondialdehyde levels, the activities of superoxide dismutase and peroxidases, chlorophyll content, and Na+/K+ ratios in leaves and roots from soybean seedlings treated with 200 mM NaCl at different time points. We found that the 200 mM NaCl treated for 12 h was optimal for undertaking a proteomic analysis on soybean seedlings. An iTRAQ-based proteomic approach was used to investigate the proteomes of soybean leaves and roots under salt treatment. These data are available via ProteomeXchange with the identifier PXD002851. In total, 278 and 440 proteins with significantly altered abundances were identified in leaves and roots of soybean, respectively. From these data, a total of 50 proteins were identified in the both tissues. These differentially expressed proteins (DEPs) were from 13 biological processes. Moreover, protein-protein interaction analysis revealed that proteins involved in metabolism, carbohydrate and energy metabolism, protein synthesis and redox homeostasis could be assigned to four high salt stress response networks. Furthermore, semi-quantitative RT-PCR analysis revealed that some of the proteins, such as a 14-3-3, MMK2, PP1, TRX-h, were also regulated by salt stress at the level of transcription. These results indicated that effective regulatory protein expression related to signaling, membrane and transport, stress defense and metabolism all played important roles in the short-term salt response of soybean seedlings.

The Arabidopsis U‐box E3 ubiquitin ligase PUB30 negatively regulates salt tolerance by facilitating BRI1 kinase inhibitor 1 (BKI1) degradation

“…Salinity is one of the major threats affecting land use capability and crop yield worldwide. Understanding the molecular mechanism governing the salt stress response will be a great asset to develop salt resistant crop plants (Ji et al, ). Salinity causes both ion toxicity and osmotic stress, and plants have developed a very complex salt tolerance mechanism during the course of evolution (Zhu, ).…”

The proteome response of “Hordeum marinum” to long-term salinity stress

“…Different types of TRxs were induced and regulated by abiotic stress in several plants (Zhang et al 2011;Chattopadhyay et al 2011;Ji et al 2016). An increase in TRx was seen in barley genotypes during salinity as well (Fatehi et al 2012a).…”

iTRAQ-based quantitative proteomic analysis of dark-germinated soybeans in response to salt stress

“…However, the mechanism of plant responses to salinity depends on the severity, duration of the stress, the growth conditions and the developmental stage of the plant, thus novel homeostasis between plant and environment at the proteome level will be established. 16,17 Up until now, little is known about the salt stress responses of darkgerminated soybeans at the proteome level. 18 Meanwhile, previous studies concerning proteomics were limited to 2dimensional gel electrophoresis (2-DE) analysis.…”

“…22 Studies have demonstrated that iTRAQ is an effective method to investigate DAPs in soybeans under different stressful physiological conditions. [23][24][25][26] To date, few studies have examined the darkgerminated soybean proteome under salt stress using iTRAQ methods.…”