Comparative proteomic analysis of seedling leaves of different salt tolerant soybean genotypes

Ma, Hongyu; Song, Liru; Shu, Yingjie; Wang, Shuang; Niu, Juan; Wang, Zhankui; Tian, Yu; Gu, Wei; Ma, Hao

doi:10.1016/j.jprot.2011.11.026

Cited by 129 publications

(77 citation statements)

References 62 publications

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“…2 and elongation factor G (spot 1810) and elongation factor Tu (spot 2610) in Longchun 23, were down-regulated, reflecting that the GTP-dependent ribosomal translocation elongation of protein biosynthesis might be inhibited by drought stress [123, 124]. The similar behaviours of these proteins were also described in soybean genotypes with different salt tolerance [125]. Ribosomal protein is one kind of highly conserved proteins that make up ribosomal subunits involved in the cellular process of translation [126].…”

Section: Discussionmentioning

confidence: 85%

Comparative proteomics illustrates the complexity of drought resistance mechanisms in two wheat (Triticum aestivum L.) cultivars under dehydration and rehydration

Cheng

Wang

et al. 2016

BMC Plant Biol

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BackgroundDrought stress is one of the most adverse environmental constraints to plant growth and productivity. Comparative proteomics of drought-tolerant and sensitive wheat genotypes is a strategy to understand the complexity of molecular mechanism of wheat in response to drought. This study attempted to extend findings regarding the potential proteomic dynamics in wheat under drought stress and to enrich the research content of drought tolerance mechanism.ResultsA comparative proteomics approach was applied to analyze proteome change of Xihan No. 2 (a drought-tolerant cultivar) and Longchun 23 (a drought-sensitive cultivar) subjected to a range of dehydration treatments (18 h, 24 h and 48 h) and rehydration treatment (R24 h) using 2-DE, respectively. Quantitative image analysis showed a total of 172 protein spots in Xihan No. 2 and 215 spots from Longchun 23 with their abundance significantly altered (p < 0.05) more than 2.5-fold. Out of these spots, a total of 84 and 64 differentially abundant proteins were identified by MALDI-TOF/TOF MS in Xihan No. 2 and Longchun 23, respectively. Most of these identified proteins were involved in metabolism, photosynthesis, defence and protein translation/processing/degradation in both two cultivars. In addition, the proteins involved in redox homeostasis, energy, transcription, cellular structure, signalling and transport were also identified. Furthermore, the comparative analysis of drought-responsive proteome allowed for the general elucidation of the major mechanisms associated with differential responses to drought of both two cultivars. These cellular processes work more cooperatively to re-establish homeostasis in Xihan No. 2 than Longchun 23. The resistance mechanisms of Xihan No. 2 mainly included changes in the metabolism of carbohydrates and amino acids as well as in the activation of more antioxidation and defense systems and in the levels of proteins involved in ATP synthesis and protein degradation/refolding.ConclusionsThis study revealed that the levels of a number of proteins involved in various cellular processes were affected by drought stress in two wheat cultivars with different drought tolerance. The results showed that there exist specific responses to drought in Xihan No. 2 and Longchun 23. The proposed hypothetical model would explain the interaction of these identified proteins that are associated with drought-responses in two cultivars, and help in developing strategies to improve drought tolerance in wheat.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-016-0871-8) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 85%

Comparative proteomics illustrates the complexity of drought resistance mechanisms in two wheat (Triticum aestivum L.) cultivars under dehydration and rehydration

Cheng

Wang

et al. 2016

BMC Plant Biol

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“…Ouyang et al , 2007; Beritognolo et al , 2011), proteomic (e.g. Ma et al , 2012; Cui et al , 2015) or metabolomic (e.g. Widodo et al , 2009; Zhao et al , 2014) levels, but limited arguments advocate the reasoning of selecting such genotypes.…”

Section: Introductionmentioning

confidence: 99%

Evaluating physiological responses of plants to salinity stress

Negrão

Schmöckel

Tester

2016

Ann Bot

912

530

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Background Because soil salinity is a major abiotic constraint affecting crop yield, much research has been conducted to develop plants with improved salinity tolerance. Salinity stress impacts many aspects of a plant’s physiology, making it difficult to study in toto. Instead, it is more tractable to dissect the plant’s response into traits that are hypothesized to be involved in the overall tolerance of the plant to salinity.Scope and conclusions We discuss how to quantify the impact of salinity on different traits, such as relative growth rate, water relations, transpiration, transpiration use efficiency, ionic relations, photosynthesis, senescence, yield and yield components. We also suggest some guidelines to assist with the selection of appropriate experimental systems, imposition of salinity stress, and obtaining and analysing relevant physiological data using appropriate indices. We illustrate how these indices can be used to identify relationships amongst the proposed traits to identify which traits are the most important contributors to salinity tolerance. Salinity tolerance is complex and involves many genes, but progress has been made in studying the mechanisms underlying a plant’s response to salinity. Nevertheless, several previous studies on salinity tolerance could have benefited from improved experimental design. We hope that this paper will provide pertinent information to researchers on performing proficient assays and interpreting results from salinity tolerance experiments.

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“…This statement has been authenticated by different workers in soybean (Sobhanian et al, 2010), rice (Liu et al, 2012), wheat (Guo et al, 2012), maize (Zörb et al, 2010), and potato (Aghaei et al, 2008). Proteome of soybean was studied under salt stress by using different tissues (Aghaei et al, 2009; Sobhanian et al, 2010; Ma et al, 2012). They identified 50S ribosome protein which was down-regulated in leaves.…”

Section: Proteomics Approachmentioning

confidence: 99%

Role of Proteomics in Crop Stress Tolerance

et al. 2016

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Plants often experience various biotic and abiotic stresses during their life cycle. The abiotic stresses include mainly drought, salt, temperature (low/high), flooding and nutritional deficiency/excess which hamper crop growth and yield to a great extent. In view of a projection 50% of the crop loss is attributable to abiotic stresses. However, abiotic stresses cause a myriad of changes in physiological, molecular and biochemical processes operating in plants. It is now widely reported that several proteins respond to these stresses at pre- and post-transcriptional and translational levels. By knowing the role of these stress inducible proteins, it would be easy to comprehensively expound the processes of stress tolerance in plants. The proteomics study offers a new approach to discover proteins and pathways associated with crop physiological and stress responses. Thus, studying the plants at proteomic levels could help understand the pathways involved in stress tolerance. Furthermore, improving the understanding of the identified key metabolic proteins involved in tolerance can be implemented into biotechnological applications, regarding recombinant/transgenic formation. Additionally, the investigation of identified metabolic processes ultimately supports the development of antistress strategies. In this review, we discussed the role of proteomics in crop stress tolerance. We also discussed different abiotic stresses and their effects on plants, particularly with reference to stress-induced expression of proteins, and how proteomics could act as vital biotechnological tools for improving stress tolerance in plants.

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Comparative proteomic analysis of seedling leaves of different salt tolerant soybean genotypes

Cited by 129 publications

References 62 publications

Comparative proteomics illustrates the complexity of drought resistance mechanisms in two wheat (Triticum aestivum L.) cultivars under dehydration and rehydration

Comparative proteomics illustrates the complexity of drought resistance mechanisms in two wheat (Triticum aestivum L.) cultivars under dehydration and rehydration

Evaluating physiological responses of plants to salinity stress

Role of Proteomics in Crop Stress Tolerance

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