Comparative Proteomic Analysis of Differentially Expressed Proteins Induced by Hydrogen Sulfide in Spinacia oleracea Leaves

Abstract

Hydrogen sulfide (H2S), as a potential gaseous messenger molecule, has been suggested to play important roles in a wide range of physiological processes in plants. The aim of present study was to investigate which set of proteins is involved in H2S-regulated metabolism or signaling pathways. Spinacia oleracea seedlings were treated with 100 µM NaHS, a donor of H2S. Changes in protein expression profiles were analyzed by 2-D gel electrophoresis coupled with MALDI-TOF MS. Over 1000 protein spots were reproducibly resolved, of which the abundance of 92 spots was changed by at least 2-fold (sixty-five were up-regulated, whereas 27 were down-regulated). These proteins were functionally divided into 9 groups, including energy production and photosynthesis, cell rescue, development and cell defense, substance metabolism, protein synthesis and folding, cellular signal transduction. Further, we found that these proteins were mainly localized in cell wall, plasma membrane, chloroplast, mitochondria, nucleus, peroxisome and cytosol. Our results demonstrate that H2S is involved in various cellular and physiological activities and has a distinct influence on photosynthesis, cell defense and cellular signal transduction in S. oleracea leaves. These findings provide new insights into proteomic responses in plants under physiological levels of H2S.

The Versatile Roles of Sulfur-Containing Biomolecules in Plant Defense—A Road to Disease Resistance

“…Sulfur has unique chemical properties because it occurs in a wide range of oxidation states (from −2 to +6) in different compounds, and hence sulfur-derived metabolites are major participants of redox metabolism and post-translational modifications as well as of detoxification processes. Two RSSs, hydrogen sulfide and sodium sulfite have been recently shown to play important roles in plant disease resistance [7,[199][200][201][202][203].…”

“…Recent studies have suggested that not H 2 S, but rather H 2 S donor sulfane sulfur compounds act as signaling molecules and are responsible for the biological activities of some RSS [207]. H 2 S influences several physiological processes, it promotes root organogenesis, seed germination, lateral root formation and enhances photosynthesis [201,203]. H 2 S treatment of plants confers protective roles in responses to heat, drought, salt, osmotic, and freezing stresses [203,205].…”

Comparative Proteomic Analysis Reveals the Regulatory Effects of H2S on Salt Tolerance of Mangrove Plant Kandelia obovata

“…Endogenous H 2 S content was determined according to the procedure of Zhang et al [62], and the method of Chen et al was employed to determine the dry weight [63].…”

Hydrogen sulfide: A novel component in Arabidopsis peroxisomes which triggers catalase inhibition

“…However, it has recently been found to have properties similar to those of multifunctional signaling molecules (NO and H 2 O 2 ) (Lisjak et al ; Gotor et al ; Hancock and Whiteman ; Li et al ; Yamasaki and Cohen ; Filipovic and Jovanovic ). As with NO and H 2 O 2, exogenous applications of H 2 S in plants have been shown to counteract the toxic effects of stresses, such as heavy metal and salinity (Zhang et al ; Chen et al , , ; Ali et al ; Bharwana et al ; Kharbech et al ; Corpas et al ). In plant systems, several enzymes are capable of generating H 2 S, which is part of the cysteine (Cys) metabolism.…”