Comparative proteome approach to characterize the high‐pressure stress response of Lactobacillus sanfranciscensis DSM 20451^T

Abstract

High hydrostatic pressure (HHP) exerts diverse effects on microorganisms, leading to stress response and cell death. While inactivation of microorganisms by lethal HHP is well investigated in the context of food preservation and the hygienic safety of minimal food processes, sublethal HHP stress response and its effect on adaptation and cross-protection is less understood. In this study, the HHP stress response of Lactobacillus sanfranciscensis was characterized and compared with cold, heat, salt, acid and starvation stress at the proteome level by using 2-DE so as to provide insight into general versus specific stress responses. Sixteen proteins were found to be affected by HHP and were identified by using N-terminal amino acid sequencing and MS. Only one slightly increased protein was specific to the HHP response and showed homology to a clp protease. The other proteins were influenced by most of the investigated stresses in a similar way as HHP. The highest similarity in the HHP proteome was found to be with cold- and NaCl-stressed cells, with 11 overlapping proteins. At the proteome level, L. sanfranciscensis appears to use overlapping subsets of stress-inducible proteins rather than stereotype responses. Our data suggest that a specific pressure response does not exist in this bacteria.

The Transcriptional Response of Lactobacillus sanfranciscensis DSM 20451 T and Its tcyB Mutant Lacking a Functional Cystine Transporter to Diamide Stress

“…T led to overexpression of proteins which had sequence homologies with heat shock proteins encoded by dnaK and groEL and with clp-encoded proteases (42). The results of the present study show that the overexpression of the molecular chaperones and proteases responds to diamide stress in a similar way in L. sanfranciscensis DSM 20451…”

Stress Physiology of Lactic Acid Bacteria

“…The system consists of DnaK, its cochaperone (DnaJ), and a nucleotide exchange factor (GrpE) (286). Expression analyses conducted at the transcript and/or protein level revealed that production of DnaK is induced in different LAB under diverse stress conditions (287)(288)(289)(290). A deletion in dnaK resulted in a thermosensitive phenotype for Lc.…”

“…GroES is the most abundant protein in B. subtilis, with some 500,000 molecules per cell after 30 min of exposure to heat stress (295). The production of the highly conserved GroEL and GroES proteins is activated under various stress conditions in LAB (287)(288)(289)(290)296).…”

Dynamic Stresses of Lactic Acid Bacteria Associated to Fermentation Processes

“…This analysis could be also performed to compare the effects that new technologies produce on bacteria comparing with the normal stress conditions. In fact, the HHP stress response of L. sanfranciscensis was compared with cold, heat, salt, acid and starvation stresses responses [18].…”

“…Several proteins are induced after high pressure treatment and some of these have also been involved in the response to other various stresses [8]. The responses to HHP stress have been studied in particular on L. sakei and L. sanfranciscensis [73,18]. These Authors suggested the presence of de novo protein synthesis as a consequence of HHP stress [73].…”