Comparative proteome analysis ofXanthomonas campestrispv.campestrisin the interaction with the susceptible and the resistant cultivars ofBrassica oleracea

Villeth, Gabriela R. C.; Reis, Fábio Bueno dos; Tonietto, Ângela; Huergo, Luciano F.; Souza, Emanuel M.; Pedrosa, F. O.; Franco, Octávio L.; Mehta, Ângela

doi:10.1111/j.1574-6968.2009.01728.x

Cited by 35 publications

(29 citation statements)

References 24 publications

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“…proteomic analysis of Xanthomonas campestris pv. campestris during its interaction with the host plant in vivo (Andrade et al ., ; Villeth et al ., ). It is clear that differences are certain to occur between bacterial interactions with the host plant in vitro and in vivo , with many processes occurring in vivo that would not occur in vitro , e.g.…”

Section: Discussionmentioning

confidence: 99%

“…The peptide masses obtained were employed for identification with the MASCOT program (Matrix Science, London, UK) using the National Center for Biotechnology Information (NCBI) and Pfam (http://pfam.sanger.ac.uk/), and only the matches to Pectobacterium species were considered as positive identifications. Peptide sequences obtained by de novo sequencing were analysed using blast (Mattinen et al ., ; Shevchenko et al ., ; Villeth et al ., ).…”

Section: Methodsmentioning

confidence: 99%

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Characterization of Pectobacterium carotovorum proteins differentially expressed during infection of Zantedeschia elliotiana in vivo and in vitro which are essential for virulence

Wang

Zhou

et al. 2016

Molecular Plant Pathology

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The identification of phytopathogen proteins that are differentially expressed during the course of the establishment of an infection is important to better understand the infection process. In vitro approaches, using plant extracts added to culture medium, have been used to identify such proteins, but the biological relevance of these findings for in planta infection are often uncertain until confirmed by in vivo studies. Here, we compared the proteins of Pectobacterium carotovorum ssp. carotovorum strain PccS1 differentially expressed in Luria-Bertani medium supplemented with extracts of the ornamental plant Zantedeschia elliotiana cultivar 'Black Magic' (in vitro) and in plant tissues (in vivo) by two-dimensional electrophoresis coupled with mass spectrometry. A total of 53 differentially expressed proteins (>1.5-fold) were identified (up-regulated or down-regulated in vitro, in vivo or both). Proteins that exhibited increased expression in vivo but not in vitro, or in both conditions, were identified, and deletions were made in a number of genes encoding these proteins, four of which (clpP, mreB, flgK and eda) led to a loss of virulence on Z. elliotiana, although clpP and mreB were later also shown to be reduced in growth in rich and minimal media. Although clpP, flgK and mreB have previously been reported as playing a role in virulence in plants, this is the first report of such a role for eda, which encodes 2-keto-3-deoxy-6-phosphogluconate (KDPG) aldolase, a key enzyme in Entner-Doudoroff metabolism. The results highlight the value of undertaking in vivo as well as in vitro approaches for the identification of new bacterial virulence factors.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Characterization of Pectobacterium carotovorum proteins differentially expressed during infection of Zantedeschia elliotiana in vivo and in vitro which are essential for virulence

Wang

Zhou

et al. 2016

Molecular Plant Pathology

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“…CAO, that converts chlorophyll a to chlorophyll b, regulates the stabilization of light-harvesting chlorophyll a/b proteins [26]. Villeth et al [27] reported that pathogen infection could decrease the accumulation of PSII stability/assembly factor HCF136, which is important for the accurate assembly of PSII. In this study, AR remarkably decreased the abundance of CAO and PSII stability/assembly factor HCF136 in L. formosana , but no light reaction-related proteins was depressed by AR in S. superba (Table 1 and 2).…”

Section: Discussionmentioning

confidence: 99%

Proteomic Analysis Reveals Differences in Tolerance to Acid Rain in Two Broad-Leaf Tree Species, Liquidambar formosana and Schima superba

Wang

Liu

et al. 2014

PLoS ONE

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Acid rain (AR) is a serious environmental issue inducing harmful impacts on plant growth and development. It has been reported that Liquidambar formosana, considered as an AR-sensitive tree species, was largely injured by AR, compared with Schima superba, an AR-tolerant tree species. To clarify the different responses of these two species to AR, a comparative proteomic analysis was conducted in this study. More than 1000 protein spots were reproducibly detected on two-dimensional electrophoresis gels. Among them, 74 protein spots from L. formosana gels and 34 protein spots from S. superba gels showed significant changes in their abundances under AR stress. In both L. formosana and S. superba, the majority proteins with more than 2 fold changes were involved in photosynthesis and energy production, followed by material metabolism, stress and defense, transcription, post-translational and modification, and signal transduction. In contrast with L. formosana, no hormone response-related protein was found in S. superba. Moreover, the changes of proteins involved in photosynthesis, starch synthesis, and translation were distinctly different between L. formosana and S. superba. Protein expression analysis of three proteins (ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit, ascorbate peroxidase and glutathione-S-transferase) by Western blot was well correlated with the results of proteomics. In conclusion, our study provides new insights into AR stress responses in woody plants and clarifies the differences in strategies to cope with AR between L. formosana and S. superba.

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“…Such approach gives valuable information on how a bacterial pathogen adapts its gene expression pattern to the plant environment. Concerning Xcc , responses to its host plant were first reported using proteome analyses (Andrade et al ., ; Villeth et al ., ). When infiltrated inside young cabbage leaves, several proteins related to polysaccharide synthesis, resistance to oxidative stress and outer membrane proteins were found to accumulate in Xcc .…”

Section: Introductionmentioning

confidence: 97%

Transcriptional reprogramming and phenotypical changes associated with growth ofXanthomonas campestrispv.campestrisin cabbage xylem sap

Bernonville

Noël

SanCristobal

et al. 2014

FEMS Microbiol Ecol

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Xylem sap (XS) is the first environment that xylem phytopathogens meet in planta during the early infection steps. Xanthomonas campestris pv. campestris (Xcc), the causative agent of Brassicaceae black rot, colonizes the plant xylem vessels to ensure its multiplication and dissemination. Besides suppression of plant immunity, Xcc has to adapt its metabolism to exploit plant-derived nutrients present in XS. To study Xcc behaviour in the early infection steps, we used cabbage XS to analyse bacterial growth. Mineral and organic composition of XS were determined. Significant growth of Xcc in XS was allowed by the rapid catabolism of amino acids, sugars and organic acids, and it was accompanied by the formation of biofilm-like structures. Transcriptome analysis of Xcc cultivated in XS using cDNA microarrays revealed a XS-specific transcriptional reprogramming compared to minimal or rich media. More specifically, up-regulation of genes encoding transporters such as TonB-dependent transporters (TBDTs), that could be associated with nutrient acquisition and detoxification, was observed. In agreement with the aggregation phenotype, expression of genes important for twitching motility and adhesion was up-regulated in XS. Taken together, our data show specific responses of Xcc to colonization of cabbage XS that could be important for the pathogenesis process and establish XS as a model medium to study mechanisms important for the early infection events.

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Comparative proteome analysis ofXanthomonas campestrispv.campestrisin the interaction with the susceptible and the resistant cultivars ofBrassica oleracea

Cited by 35 publications

References 24 publications

Characterization of Pectobacterium carotovorum proteins differentially expressed during infection of Zantedeschia elliotiana in vivo and in vitro which are essential for virulence

Characterization of Pectobacterium carotovorum proteins differentially expressed during infection of Zantedeschia elliotiana in vivo and in vitro which are essential for virulence

Proteomic Analysis Reveals Differences in Tolerance to Acid Rain in Two Broad-Leaf Tree Species, Liquidambar formosana and Schima superba

Transcriptional reprogramming and phenotypical changes associated with growth ofXanthomonas campestrispv.campestrisin cabbage xylem sap

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