Comparative properties of schistosomal and filarial dihydrofolate reductases

Jaffe, Julian J.; McCormack, John J.; Meymarian, Euphronia

doi:10.1016/0006-2952(72)90064-0

Cited by 41 publications

(15 citation statements)

References 35 publications

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“…However, other folic acid pathway elements, e.g., dihydrofolate reductase, which has been characterized in flatworms [20,21], were not found in this study.…”

Section: Resultsmentioning

confidence: 56%

New Perspectives on Host-Parasite Interplay by Comparative Transcriptomic and Proteomic Analyses of Schistosoma japonicum

et al. 2006

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Schistosomiasis remains a serious public health problem with an estimated 200 million people infected in 76 countries. Here we isolated ~ 8,400 potential protein-encoding cDNA contigs from Schistosoma japonicum after sequencing circa 84,000 expressed sequence tags. In tandem, we undertook a high-throughput proteomics approach to characterize the protein expression profiles of a number of developmental stages (cercariae, hepatic schistosomula, female and male adults, eggs, and miracidia) and tissues at the host-parasite interface (eggshell and tegument) by interrogating the protein database deduced from the contigs. Comparative analysis of these transcriptomic and proteomic data, the latter including 3,260 proteins with putative identities, revealed differential expression of genes among the various developmental stages and sexes of S. japonicum and localization of putative secretory and membrane antigens, enzymes, and other gene products on the adult tegument and eggshell, many of which displayed genetic polymorphisms. Numerous S. japonicum genes exhibited high levels of identity with those of their mammalian hosts, whereas many others appeared to be conserved only across the genus Schistosoma or Phylum Platyhelminthes. These findings are expected to provide new insights into the pathophysiology of schistosomiasis and for the development of improved interventions for disease control and will facilitate a more fundamental understanding of schistosome biology, evolution, and the host-parasite interplay.

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“…However, other folic acid pathway elements, e.g., dihydrofolate reductase, which has been characterized in flatworms [20,21], were not found in this study.…”

Section: Resultsmentioning

confidence: 56%

New Perspectives on Host-Parasite Interplay by Comparative Transcriptomic and Proteomic Analyses of Schistosoma japonicum

et al. 2006

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“…been observed for all protozoal organisms studied in this regard but not in most other organisms studied, including representatives of bacteria (1), yeast (25), worms (26), insects (27), and vertebrates (1). P. berg/wi thymidylate synthase coelutes with H2folate reductase from a Sephadex G-200 column and, most importantly, is eluted from a methotrexate-Sepharose affinity column by conditions usually specific for H2folate reductase only.…”

Section: Resultsmentioning

confidence: 99%

Dihydrofolate reductase: thymidylate synthase, a bifunctional polypeptide from Crithidia fasciculata.

Ferone¹,

Roland²

1980

Proc. Natl. Acad. Sci. U.S.A.

109

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“…Because the available evidence suggested that normal mammalian tissues, blood and urine do not contain measurable quantities of thymine (Cleaver, 1967;Jaffe, McCormack and Meymarian, 1972;Then and Angehm, 1974), the emergence of such mutants in vivo during trimethoprim therapy seemed unlikely. However, thy-mutants of several organisms have been isolated from patients treated with co-trimoxazole, and we have demonstrated that, in six such patients, the urine contained a thyminelike substance.…”

Section: Discussionmentioning

confidence: 99%