Comparative phytochemical and in vitro antimicrobial activities of the leaf extracts of two medicinal plants growing in North-East, Nigeria

Usman, Hadiza Abdullahi; Tijjani, Mustapha A; Hassan, Abudlkarim; Aji, Zainab Babagana

doi:10.15171/jhp.2018.11

Cited by 7 publications

(5 citation statements)

References 12 publications

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“…The presence of essential oil, an alkaloid, curcumins and turmerol and valeric acid are responsible for antibacterial action of spices which involve the hydrophobic and hydrogen bonding of phenolic compounds to membrane proteins, membrane disruption and destruction of electron transport system and cell wall disruption. According to [Tijjani. et al,2017], an antimicrobial agent which the diameter of the zone of inhibition is above 3 mm, the organism is said to be sensitive but if it is 2 mm or less than that, the organism is said to have resistance over the particular agent.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial activities of aqueous and ethanolic extracts of some natural spices (garlic, turmeric, thyme and onions) on some clinical isolates

Omorodion¹,

Ujoh²

2022

GSC Biol. and Pharm. Sci.

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Different types of spices used daily have been documented to have antimicrobial and medicinal value as well. Most bacteria are sensitive to the extracts from plants such as, garlic, onion, thyme and turmeric etc. Spices such as these have been used as antimicrobial agents in their raw form for the treatment of wounds, injuries and joint pains etc. The present study was conducted to investigate the antibacterial activity of these natural spices. Different concentrations of extracts were prepared by using two solvents; water and ethanol, the test organisms used were Escherichia coli and Staphylococcus aureus. All the spices used were effective against the test organisms, but the best activity was shown in garlic, while the least activity was shown in thyme, in general, the ethanolic extracts were more reactive than the aqueous extracts. Natural spices could be a potential source for antimicrobial agents. Validation of natural spices as alternative and complementary medicine can be achieved by several in vivo trials through which the safe use of such products can be assured. This study emphasizes the therapeutic potential of spices and the need to enhance its exploitation on this regard.

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Section: Discussionmentioning

confidence: 99%

Antimicrobial activities of aqueous and ethanolic extracts of some natural spices (garlic, turmeric, thyme and onions) on some clinical isolates

Omorodion¹,

Ujoh²

2022

GSC Biol. and Pharm. Sci.

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“…The lowest concentrations of scorpion venom that has an antibacterial effect on the organism are determined as the minimum bactericidal concentration (MBC). This will be determined from the broth dilution resulting from MIC tubes by sub-culturing to the surface of freshly prepared nutrient agar plates by using a sterile inoculating loop to streak the plate as described by Vollekova et al, (2001) and Usman et al, (2007). All the test tubes that showed no microbial growth after 24 hours of incubation i.e.…”

Section: Determination Of Minimum Bactericidal Concentration (Mbc)mentioning

confidence: 99%

Assessment of antibacterial efficacy and toxicological implications of emperor scorpion (Pandinus imperator) venom using animal model

Ayodeji Adams DAHUNSI,

Bukola Catherine AKIN-OSANAIYE

2024

World J. Adv. Pharm. Life Sci.

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This research work was carried out by collecting 50 Pandinus imperator Scorpions and ascetically extracting venom locally from them and testing the venom on some selected bacteria. The extracted venom used was both in the lyophilized and crude (non-lyophilized) state to determine their different efficacy and was passed through a membrane filter to ensure sterility. Bacterial (Pseudomonas aeruginosa, Escherichia coli, and Acetinibacter baumannii) were isolated from drinking water sources of the University of Abuja after which it was preserved in slant for further use. Serotyped bacteria were also obtained and used in this study. Appropriate differential and selective agar were used to culture and sub-culture the isolates and serotyped while Analytical Profile Index (API) kits were used to confirm the isolated bacteria. A sensitivity test was carried out on each bacterium with the venom at concentrations of 25%, 50%, 75%, and 100% for crude venom while 10, 20, 30, and 40 mg/ml for the lyophilized venom, minimum inhibitory concentration, and minimum bactericidal concentration were also determined. The result shows lowest zone for crude venom against isolated bacteria was 3mm at 25% against Acinetobacter baumanni while the highest was 11.5 mm at 100% against Pseudomonas aeruginosa, the lowest for crude venom against serotyped was 4 mm at 25% against Acinetobacter baumanni and highest was 12.2 mm at 100% against Pseudomonas aeruginosa, lowest zone for lyophilized venom against isolated bacteria was 2.8 mm at 10 mg/ml against Acinetobacter baumanni while highest was 10.8 mm at 40 mg/ml against Pseudomonas aeruginosa, also the lowest for lyophilized venom serotyped was 3.8 mm at 10 mg/ml against Acinetobacter baumanni and highest was 11.5 mm at 40 mg/ml against Pseudomonas aeruginosa. The lowest MIC was 25% and 20 mg/ml while the highest was 50% and 30 mg/ml, the lowest MBC was 50% and 30 mg/ml while the lowest was 75% and 40 mg/ml. The toxicity of the venom was carried out by testing the venom on mice which shows no harmful effect on them. ANOVA statistical analysis carried out showed no significant difference in the activities of both venom states.

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“…9,12,15-Octadecadienoic acid has a retention time of 4.08, and a peak area of 6.78 is used as an Anti-inflammatory, anti-asthma, antimicrobial, antioxidant, anticancer, anti-arthritic, Hepatoprotective and diuretic (Salah et al, 2015). Ethyl tridecanoate, with a retention time of 5.096 and a peak area of 4.3, is used as antioxidant, antimicrobial and anticancer activities (Usman et al, 2018). Dodecanoic acid, with a retention time of 11.432 and a peak area of 1.37, is used as an antibacterial and antifungal agent (Seidel & Taylor, 2004).…”

Section: Escherichia Colimentioning

confidence: 99%

Antibacterial, phytochemical and GC-MS analysis of Thevetia peruviana extracts: An approach in drug formulation

Edo¹

2022

NRFHH

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Thevetia peruviana is a large flowering shrub in the Apocynaceae family used medicinally. The current study aims to determine the phytochemicals and antimicrobial potential of T. peruviana. GC-MS was used to screen the ethanol and n-hexane extracts. Leaf extracts (ethanol and hexane) revealed alkaloids, phenols, anthraquinones, cardiac glycosides, flavonoids, tannins, steroid glycosides, carbohydrates, proteins, terpenoids, terpenoids and fixed oils and fats. GC-MS analysis of the ethanol and hexane extract revealed 24 bioactive metabolites. T. peruviana leaf extracts inhibited tested pathogens at 50, 100, and 200 mg/ml concentrations. A plethora of secondary metabolites demonstrated promising pharmacological benefits. The bioactive chemicals are utilised to treat bacterial infections, cancer, diabetes, and inflammation. This study demonstrated the antibacterial activity of several plants used in traditional medicine.

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Comparative phytochemical and in vitro antimicrobial activities of the leaf extracts of two medicinal plants growing in North-East, Nigeria

Cited by 7 publications

References 12 publications

Antimicrobial activities of aqueous and ethanolic extracts of some natural spices (garlic, turmeric, thyme and onions) on some clinical isolates

Antimicrobial activities of aqueous and ethanolic extracts of some natural spices (garlic, turmeric, thyme and onions) on some clinical isolates

Assessment of antibacterial efficacy and toxicological implications of emperor scorpion (Pandinus imperator) venom using animal model

Antibacterial, phytochemical and GC-MS analysis of <i>Thevetia peruviana</i> extracts: An approach in drug formulation

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