Comparative pharmacokinetics of seven bioactive components after oral administration of crude and processed Qixue Shuangbu Prescription in chronic heart failure rats by microdialysis combined with UPLC-MS/MS

Exploring the Efficacy Enhancement Mechanism of Qixue Shuangbu prescription after TCM processing for treating chronic heart failure by regulating ERK/Bcl-2/Bax/Caspases-3 signaling pathway

“…There were 101 common peaks of compounds in both CQSP and PQSP, in which 31 analytes increased and 38 analytes reduced in PQSP. The pharmacokinetic parameters of main bioactive components indicated longer lasting time elimination, better bioavailability and absorption for enhancing the effect in the treatment of CHF in the PQSP group than that of the CQSP group [ 17 , 26 ].…”

“…Nine crude Chinese medicine decoction pieces of QSP were supplied by the Affiliated Taizhou People's Hospital of Nanjing Medical University, and authenticated by Dr. Linwei Chen. The CQSP and PQSP were prepared according to our previous studies [ [15] , [16] , [17] ]. Benazepril hydrochloride tablet (BHT) was purchased from Beijing Novartis Pharmaceutical Co., Ltd (Beijing, China).…”

Synergistic mechanism of processing method for Qixue Shuangbu prescription in the treatment of chronic heart failure based on plasma metabolomics—Systematic bioinformatics

“…In contrast, processed QSP is composed of Ren Shen (3 g), Huang Qi (stir‐frying with honey; 2 g), Dang Gui (stir‐frying with yellow rice wine; 2 g), Bai Shao (stir‐frying with yellow rice wine; 2 g), Gou Qi Zi (2 g), He Shou Wu (steaming with black bean extract; 2 g), Yu Zhu (2 g), Huang Jing (steaming with yellow rice wine; 2 g), and Chen Pi (1 g). Herbal decoction pieces of crude and processed QSP were soaked in 10 times the amount of water and boiled for 2 h, and filtered through filter paper, respectively [8]. The quality control of QSP decoction was carried out by HPLC fingerprinting, as shown in Supporting Information Figure S1.…”

“…All blood samples were immediately centrifuged at 12 000 rpm for 5 min. Also, a total of 500 μL of the supernatant was vortexed with 1500 μL acetonitrile for 1 min and centrifuged at 12 000 rpm for 10 min (4 • C) to precipitate the protein [8]. The supernatant was collected and evaporated till dry under a nitrogen atmosphere at 40 • C. The residue was fully dissolved in 100 μL acetonitrile by vortex mixing and centrifuged at 12 000 rpm for 10 min (4 • C).…”

“…[7]. We further studied the pharmacokinetics of seven bioactive constituents after oral administration of QSP in CHF pathological conditions [8]. However, the absorbed constituents and synergistic mechanisms of processed QSP for enhancing the therapeutic effect remain unclear.…”