Comparative pharmacokinetics of enrofloxacin and ciprofloxacin in lactating dairy cows and beef steers following intravenous administration of enrofloxacin

Idowu, O. A.; Peggins, James O.; Cullison, R.; Bredow, J. von

doi:10.1016/j.rvsc.2009.12.019

Cited by 42 publications

(36 citation statements)

References 15 publications

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“…All C. jejuni strains had a nucleotide 257 change from ACA to ATA and all C. coli strains had a change from ACT to ATT as described previously (SAID et al, 2010). Ciprofloxacin is the main metabolite of enrofloxacin and the antimicrobial activity of the latter has been linked in part to the action of this metabolite (IDOWU et al, 2010). Although previous research addressed only the mutations found in ciprofloxacin-resistant strains, in this study the strains were also resistant to enrofloxacin, suggesting the same resistance acquisition mechanism.…”

Section: Discussionmentioning

confidence: 83%

Detection of fluoroquinolone resistance by mutation in gyrA gene of Campylobacter spp. isolates from broiler and laying (Gallus gallus domesticus) hens,from Rio de Janeiro State, Brazil

et al. 2015

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Section: Discussionmentioning

confidence: 83%

Detection of fluoroquinolone resistance by mutation in gyrA gene of Campylobacter spp. isolates from broiler and laying (Gallus gallus domesticus) hens,from Rio de Janeiro State, Brazil

et al. 2015

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“…Para enrofloxacina, 42,50% das cepas foram resistentes (CIM ≥4-32µg/ mL) e 38,75% apresentaram resistência intermediária (CIM =2µg/mL), demonstrando um nível de resistência inferior ao detectado para ciprofloxacina. A maior resistência à ciprofloxacina observada nesse estudo pode ser explicada pelo fato da ciprofloxacina ser considerada um metabólito da enrofloxacina (Idowu et al 2010). Com o uso frequente da enrofloxacina na avicultura, na metabolização deste antimicrobiano, cepas resistentes à ciprofloxacina podem ter sido selecionadas.…”

Section: Discussionunclassified

Detecção de resistência às fluoroquinolonas em Campylobacter isolados de frangos de criação orgânica

et al. 2015

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ABSTRACT.-Frasão B.S., Côrtes L.R., Nascimento E.R., Cunha N.C., Almeida V. Studies have shown that resistance to quinolones in Campylobacter strains is related with Threonine-86-Isoleucine mutation. In order to investigate the presence of this mutation in sensitive and resistant Campylobacter strains to ciprofloxacin and enrofloxacin, the cecal contents of 80 broilers from organic raising chickens, slaughtered under State Inspection Service (S.I.S) of the State of Rio de Janeiro, were collected and tested for the presence of Campylobacter. The determination of ciprofloxacin and enrofloxacin susceptibility was done by disk diffusion and agar dilution methods for determining the Minimum Inhibitory Concentration (MIC). The detection of mutation in Quinolone Resistance Determinant Region (QRDR) in gyrA gene was done by sequencing. Campylobacter was isolated from 100% of the samples, being 68.75% C. jejuni and 31.25% C. coli. By the disk diffusion method, resistance to ciprofloxacin was observed in all isolates and 56.25% of the strains were resistant to enrofloxacin. By agar dilution method, all strains were resistant to ciprofloxacin (MIC ≥ 16µg/mL to ≥ 64µg/mL) and full and intermediate resistance to enrofloxacin was detected in 42.50% (MIC ≥ 4-32μg/mL) and 38.75% (MIC =2μg/mL) of the strains, respectively. Mutation Thr-86-Ile was observed in 100% of the isolates investigated. In addition to this mutation, others no silent mutations and silent mutations (His-81-His, Ser-119-Ser, Ala-120-Ala, Phe-99-Phe, Ala-122-Ala, Gly-74-Gly, Ile-77-Ile, Ala-91-Ala, Leu-92-Leu, Val-93-Val, Ile-106-Ile, Thr-107-Thr, Gly-113-Gly, Ile-115-Ile, Gly-110-Gly) were detected. All the enrofloxacin--sensitive strains by the phenotypic methods had the Thr-86 to Ile substitution, which suggests other mechanisms contributing to enrofloxacin resistance in Campylobacter.

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“…This was complemented with a direct comparison of measured environmental concentrations and VICH action limits with the CO WT of species represented in the MIC distributions of each antibiotic. Although ciprofloxacin is not approved for use in veterinary medicine, it is the major active metabolite of enrofloxacin in different species (Idowu et al 2010). In the absence of data for enrofloxacin, we used ciprofloxacin as a representative of the fluoroquinolones in comparisons of potentially affected fractions and VICH phase I action limits.…”

Section: Methodsmentioning

confidence: 99%

Selective Pressure of Antibiotic Pollution on Bacteria of Importance to Public Health

Tello

Austin

Telfer

2012

Environ Health Perspect

282

138

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Background: Many bacteria of clinical importance survive and may grow in different environments. Antibiotic pollution may exert on them a selective pressure leading to an increase in the prevalence of resistance.Objectives: In this study we sought to determine whether environmental concentrations of antibiotics and concentrations representing action limits used in environmental risk assessment may exert a selective pressure on clinically relevant bacteria in the environment.Methods: We used bacterial inhibition as an assessment end point to link antibiotic selective pressures to the prevalence of resistance in bacterial populations. Species sensitivity distributions were derived for three antibiotics by fitting log-logistic models to end points calculated from minimum inhibitory concentration (MIC) distributions based on worldwide data collated by the European Committee on Antimicrobial Susceptibility Testing (EUCAST). To place bacteria represented in these distributions in a broader context, we performed a brief phylogenetic analysis. The potentially affected fraction of bacterial genera at measured environmental concentrations of antibiotics and environmental risk assessment action limits was used as a proxy for antibiotic selective pressure. Measured environmental concentrations and environmental risk assessment action limits were also directly compared to wild-type cut-off values.Results: The potentially affected fraction of bacterial genera estimated based on antibiotic concentrations measured in water environments is ≤ 7%. We estimated that measured environmental concentrations in river sediments, swine feces lagoons, liquid manure, and farmed soil inhibit wild-type populations in up to 60%, 92%, 100%, and 30% of bacterial genera, respectively. At concentrations used as action limits in environmental risk assessment, erythromycin and ciprofloxacin were estimated to inhibit wild-type populations in up to 25% and 76% of bacterial genera.Conclusions: Measured environmental concentrations of antibiotics, as well as concentrations representing environmental risk assessment action limits, are high enough to exert a selective pressure on clinically relevant bacteria that may lead to an increase in the prevalence of resistance.

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Comparative pharmacokinetics of enrofloxacin and ciprofloxacin in lactating dairy cows and beef steers following intravenous administration of enrofloxacin

Cited by 42 publications

References 15 publications

Detection of fluoroquinolone resistance by mutation in gyrA gene of Campylobacter spp. isolates from broiler and laying (Gallus gallus domesticus) hens,from Rio de Janeiro State, Brazil

Detection of fluoroquinolone resistance by mutation in gyrA gene of Campylobacter spp. isolates from broiler and laying (Gallus gallus domesticus) hens,from Rio de Janeiro State, Brazil

Detecção de resistência às fluoroquinolonas em Campylobacter isolados de frangos de criação orgânica

Selective Pressure of Antibiotic Pollution on Bacteria of Importance to Public Health

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