Comparative pathology of lambs and calves infected with parainfluenza virus type 3

Stevenson, R.G.; Hore, D. E.

doi:10.1016/0021-9975(70)90060-5

Cited by 24 publications

(20 citation statements)

References 8 publications

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“…In experimental PI-3 virus infection in calves and lambs, fluorescent foci were determined by DFAT widely in alveoli around the bronchoalveolar epithelium, these areas were referred to as starting points for histopathological changes (Stevenson, 1970). Similarly, in all examined lungs the fluorescent staining was detected in the cytoplasm.…”

Section: Resultsmentioning

confidence: 99%

Detection of Parainfluenza Type 3 Virus Antigens in Goats

Aniţă

Raileanu

et al. 2015

BUASVMCN-VM

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Parainfluenza-3 (PI-3) is the most common virus infection of the respiratory tract of sheep and goats throughout the world. The virus belongs to family Paramyxoviridae of order Mononegaviralis, the non-segmented negative single stranded RNA viruses. Often, infection is complicated by coinfection with respiratory bacteria (Mannheimia haemolytica). In this study, detection of PI-3 virus as causative agent of pneumonia in goat kids was investigated.The virus was detected in lung tissues using direct immunofluorescence method (DFAT) with anti-PI-3 polyclonal antiserum conjugated to fluorescein isothiocyanate (VMRD, cat. no. CJ-F-PI3-10ML). The samples were collected from twelve diseased goat kids from a farm where were diagnosed respiratory signs.The outbreak dynamics was characterized by high morbidity and mortality in goat kids aged between one to four weeks. Clinically all affected animals were presenting respiratory manifestation (coughing and nasal discharge). At the necropsy pneumonic apical and cardiac lobes in lungs were observed in all died goat kids. The positive result of DFAT was considered based on the specific fluorescence localized in the cytoplasm of bronchiolar epithelial cells.In the present research work, we demonstrated the suitability of the immunodetection of viral antigens in routinely fixed tissue specimens as a diagnostic tool for PI-3 infection in goats.

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Section: Resultsmentioning

confidence: 99%

Detection of Parainfluenza Type 3 Virus Antigens in Goats

Aniţă

Raileanu

et al. 2015

BUASVMCN-VM

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“…The host-specific sequence elements might contribute to the growth of the virus in heterogenous hosts [37]. Cross-species infection of BPIV3 in a human [54], BPIV3 in sheep, and ovine PIV-3 in cattle [55] have been reported. This happens to be the first report of cross-species infection of BPIV3 in pigs.…”

Section: Phylogenetic Analysismentioning

confidence: 98%

Molecular characterization of glycoprotein genes and phylogenetic analysis of two swine paramyxoviruses isolated from United States

2009

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Two swine paramyxoviruses (SPMV)-(81-19252 (Texas-81) and 92-7783 (ISU-92)-were isolated from encephalitic pigs in the United States in 1981 and 1992. Antigenic, morphologic, and biological characteristics of these two viruses were essentially similar to members of the family Paramyxoviridae. Antigenic analysis by indirect fluorescent antibody, immunoblot, and one-way cross-neutralization tests placed these viruses along with bovine parainfluenza 3 (BPIV3) viruses. Purified virions were 50-300 nm in size and morphologically indistinguishable from other paramyxoviruses. These two viruses hemagglutinated red blood cells and had neuraminidase activity. The gene junctions of fusion (F) and hemagglutinin (HN) glycoprotein genes of these viruses contained highly conserved transcription start and stop signal sequences and trinucleotide intergenic regions similar to other Paramyxoviridae. The F gene of ISU-92 was longer than Texas-81 due to insertion of a 24-nucleotide "U"-rich 3' untranslated region. Structure-based sequence alignment of glycoproteins of these two SPMVs indicated that they are essentially similar in structure and function to parainfluenzaviruses. The Texas-81 strain was closely related to BPIV3 Shipping Fever (SF) strain at nucleotide and amino acid level, while the ISU-92 strain was more closely related to BPIV3 910N strain. The envelope glycoproteins of ISU-92 had only approximately 92 and approximately 96% identity at nucleotide and amino acid levels with BPIV3-SF strain, respectively. The high sequence identities to BPIV3 indicated cross-species infection in pigs. Phylogenetic analyses based on both F protein and HN protein suggested the classification of these viruses into the subfamily Paramyxovirinae, genus Respirovirus, and genotype A of BPIV3.

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“…PIV-3 infections have been serologically demonstrated in a wide variety of mammals including cattle, humans, sheep (Lyon et al, 1997), goats (Yener et al, 2005), bison (Zarnke & Erickson, 1990), guinea pigs (Ohsawa et al, 1998), black and white rhinoceros (FischerTenhagen et al, 2000), moose (Thorsen & Henderson, 1971), bighorn sheep (Parks et al, 1972) and camels (Eisa et al, 1979). Cross-species infections have been reported in numerous instances including HPIV-3 in guinea pigs (Ohsawa et al, 1998), BPIV-3 in a human (Ben-Ishai et al, 1980), BPIV-3 in sheep and ovine PIV-3 in cattle (Stevenson & Hore, 1970). Further sequence analysis of BPIV-3 isolates from a larger number of host species will be essential to fully elucidate the relationship between the various host strains of PIV-3.…”

Section: Identification Of Two Bpiv-3 Genotypesmentioning

confidence: 99%

Identification of two distinct bovine parainfluenza virus type 3 genotypes

Horwood¹,

Gravel²,

Mahony³

2008

Journal of General Virology

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The partial gene sequencing of the matrix (M) protein from seven clinical isolates of bovine parainfluenza virus type 3 (BPIV-3), and the complete sequencing of a representative isolate (Q5592) was completed in this study. Nucleotide sequence analysis was initiated because of the failure of in-house BPIV-3 RT-PCR methods to yield expected products for four of the isolates. Phylogenetic reconstructions based on the nucleotide sequences for the M-protein and the entire genome, using all of the available BPIV-3 nucleotide sequences, demonstrated that there were two distinct BPIV-3 genotypes (BPIV-3a and BPIV-3b). These newly identified genotypes have implications for the development of BPIV-3 molecular detection methods and may also impact on BPIV-3 vaccine formulations.

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Comparative pathology of lambs and calves infected with parainfluenza virus type 3

Cited by 24 publications

References 8 publications

Detection of Parainfluenza Type 3 Virus Antigens in Goats

Detection of Parainfluenza Type 3 Virus Antigens in Goats

Molecular characterization of glycoprotein genes and phylogenetic analysis of two swine paramyxoviruses isolated from United States

Identification of two distinct bovine parainfluenza virus type 3 genotypes

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